Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 50 issue 5 (october 2016) : 674-678

Ultrastructural features of atretic ovarian follicles in buffalo (Bubalus bubalis)

Sreejalekshmi P.1, Prasad R.V.1, Selvaraju S.*, Jamuna K.V.1, Sivagnanam S.1
1<p>ICAR-National Institute of Animal Nutrition and Physiology,&nbsp;Adugodi, Bangalore-560 030, India.</p>
Cite article:- P.1 Sreejalekshmi, R.V.1 Prasad, S.* Selvaraju, K.V.1 Jamuna, S.1 Sivagnanam (2016). Ultrastructural features of atretic ovarian follicles in buffalo (Bubalus bubalis) . Indian Journal of Animal Research. 50(5): 674-678. doi: 10.18805/ijar.9366.

ABSTRACT

The process of follicular development and features of morphological changes are essential to understand the factors involved during folliculogenesis and to improve fertility in buffalo. Hence the objective of the present study was to report ultrastructural features of atretic ovarian follicles in buffalo. In the early stage of atresia, the involvement of mitochondria was observed and whereas in the later stages of atresia, involvement of macrophages were observed. The granulosa cells of buffalo atretic tertiary follicles were observed to contain undulation of the nuclear membrane, crescent formation of the pyknotic nuclei and loopiness and undulations of the basal lamina. These features are similar to the reports in other species. The involvement of mitochondria in the early stage of atresia suggested the probable role of a reactive oxygen free radical induced damage.

REFERENCES


  1. Aboul-Ela M.B.E. (2000). Super-ovulation in the buffalo: constraints and manipulation. Buffalo J. 1: 1–20. 

  2. Arends, M.J., Morris, R.G. and Wyllie, A.H. (1990). Apoptosis: the role of the endonuclease. Am J Pathol. 136: 593–608.

  3. Bancroft, J.D. and Stevens, A. (1996). Theory and Practice of Histological Techniques. 4th Ed. Churchill Livingstone, New York.

  4. Blondin, P., Dufour, M. and Sirard, M. A. (1996). Analysis of atresia in bovine follicles using different methods: flow cytometry, enzyme-linked immunosorbent assay, and classic histology. Biol Reprod. 54: 631-7.

  5. De Pol, A., Vaccina F., Forabosco A., Cavazzuti, E. and Marzona, L. (1997). Apoptosis of germ cells during human prenatal oogenesis. Human Reprod. 12: 2235–2241.

  6. Devine, P.J., Payne, C.M., McCuskey, M.K. and Hoyer, P.B. (2000). Ultrastructural evaluation of oocyte during atresia in rat ovarian follicles. Biol Reprod. 63: 1245-1252.

  7. Feranil, J.B., Isobe, N. and Nakao, T. (2004). Cell proliferation in the atretic follicles of buffalo and cattle ovary. Reprod Dom Anim. 39: 405 – 409

  8. Hsueh, A.J.W., Billig, H. and Tsafriri, A. (1994). Ovarian follicle atresia: a hormonally controlled apoptotic process. Endo Reviews. 15: 707–724.

  9. Hughes, R.M. and Gorospe, W.G. (1991). Biochemical identification of apoptosis (programmed cell death) in granulosa cells: evidence for a potential mechanism underlying follicular atresia. Endocrinology. 129: 2415–2422.

  10. Ireland, J.J., Murphe, R.L. and Coulson, P.B. (1980). Accuracy of predicting stages of bovine estrous cycle by gross appearance of the corpus luteum. J Dairy Sci. 63: 155-160

  11. Irving-Rodgers, H.F. and Rodgers, R.J. (2000). Ultrastructure of the bovine ovarian follicles and its relationship to the membrana granulosa. J Reprod Fertil. 118: 221-228.

  12. Irving-Rodgers, H.F., Van Wezel, I.L., Mussard, M.L., Kinder, J.E. and Rodgers, R.J. (2001). Atresia revisited: two basic patterns of atresia of bovine antral follicles. Reproduction (Cambridge)., 122: 761-775.

  13. Kaipia, A. and Hsueh, A. J.W. (1997). Regulation of ovarian follicle atresia. Annual Rev Physiol. 59: 349–363.

  14. Kalekar, A. and Thompson, C.B. (1998). Bcl-2 protein: the role of BH3 domains in apoptosis. Trends in Cell Biol. 8: 324-329.

  15. Keefe, D.I., Niven-Fairchild, T., Powell, S. and Buradagunta, S. (1995). Mitochondrial deoxyribonucleic acid deletions in oocytes and reproductive aging in women. Fertil Steril. 64: 577-583.

  16. Kitagawa, T., Suganuma, N., Nawa, A., Kikkawa, F., Tanaka, M., Ozawa, T. and Tomoda, Y. (1993). Rapid accumulation of deleted mitochondrial deoxyribonucleic acid in post menopausal ovaries. Biol Reprod. 49: 730-736.

  17. Ortiz, R., Echeverria, O. M., Salgado, R., Escobar, M.L. and Vazquez-Nin, G.H. (2006). Fine structural and cytochemical analysis of the processes of cell death of oocytes in atretic follicles in new born and prepubertal rats. Apoptosis. 11: 25-37.

  18. Park, C.S., Han, S.R., Kim, S.I., Cho, K.J. and Kim, W.S. (2004). A morphological study on the granulosa cell apoptosis and macrophages during follicular atresia in pig ovary. J Anim Sci Tech. 46: 571-584.

  19. Peluso, J. J., England-Charlesworth, C., Bolender, D. L. and Steger, R, W. (1980).Ultrastructural alterations associated with the initiation of follicular atresia. Cell and Tissue Res. 211: 105-115.

  20. Selvaraju, S., Raghavendra, B., Siva Subramani, T., Priyadharsini, R., Reddy, I.J. and Ravindra, J.P. (2010). Changes in luteal cells distribution, apoptotic rate, lipid peroxidation levels and antioxidant enzyme activities in buffalo (Bubalus bubalis) corpus luteum, Anim Reprod Sci. 120:39-46.

  21. Silva, J. R.V., Bao, S.N., Lucci, C.M., Carvalho, F.C.A., Andrade, E.R., Ferreira, M. A.L. and Figueiredo, J.R. (2001). Morphological and ultrastructural changes occurring during degeneration of goat preantral follicles preserved in vitro. Anim Reprod Sci. 66: 209-23.

  22. Sreejalekshmi, P., Raghavendra, B.S., Siva Subramani, T., Chandrashekara Murthy, V., Jamuna, K.V., Prasad, R.V., Ravindra, J.P. and Selvaraju, S. (2011). Detection of follicular apoptosis in water buffalo (Bubalus bubalis) ovary by histology and nick end labelling technique. Reprod Dom Anim. 46: 59-65

  23. Susin, S. A., Zamazami, N., Castedo, M., Hirsch, T., Marchetti, P., Macho, A., Daugas, E., Geuskens, M. and Kroemer, G. (1996). Bcl-2 inhibits mitochondrial release of an apoptogenic protease. J Exp Med. 184: 1331-1341

  24. Sugimoto, M., Manabe, N., Kimura, Y., Myoumoto, A., Imai, Y., Ohno, H. and Miyamoto, H. (1998). Ultrastructural changes in granulosa cells in porcine antral follicles undergoing atresia indicate apoptotic cell death. J Reprod Dev. 44: 7-14.

  25. VanWezel, I. L., Dharmarajan, A.M., Lavranos, T. C. and Rodgers, R. J. (1999). Evidence for alternative pathways of granulosa cell death in healthy and slightly atretic bovine antral follicles. Endocrinology. 140: 2602-2612.

  26. Yang, M. Y. and Rajamahendran, R. (2000). Morphological and biochemical identification of apoptosis in small, medium, and large bovine follicles and the effects of follicle-stimulating hormone and insulin-like growth factor-1 on spontaneous apoptosis in cultured bovine granulosa cells. Biol Reprod. 62: 1209-1217.

     
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)