In Vitro maturation rate of oocytes collected from indigenous cows of Assam after slaughter

DOI: 10.18805/ijar.7054    | Article Id: B-2757 | Page : 869-871
Citation :- In Vitro maturation rate of oocytes collected from indigenous cowsof Assam after slaughter .Indian Journal Of Animal Research.2015.(49):869-871

S.S. Deka, D. J. Kalita*, S. Sarma and D.J. Dutta

djkalita@rediiffmail.com
Address :

Department of Veterinary Biochemistry, College of Veterinary Science, Assam Agricultural University, Guwahati-781 022, India.

Abstract

Sixty healthy ovaries were collected from local slaughter house. Oocytes from small and medium sized follicles (2-8 mm in diameter) were selected for oocyte collection using aspiration followed by slicing . A and B category ( cumulus oocyte complexes (COCs) with more than 5 layers and  3-5 layers respectively of compact cumulus cells surrounding the zona pellucida) were selected for in vitro maturation. A total of 361 oocytes were found to be matured with  overall mean maturation rate of 82.59 ± 0.02%. The mean number of grade A, B and C oocytes recovered per ovary was 4.26 ± 0.53, 3.05 ± 0.31 and 1.25 ± 0.19, respectively. The overall recovery of grade A, B and C oocytes were 256, 183 and 74 . Out of 439  oocytes 58.86 ± 0.05%,  33.23 ±0.04 % and 9.90 ± 0.04 %  showed +++, ++ and +  degrees of cumulus expansion, respectively. It was concluded that  aspiration followed by slicing is an ideal method for maximum yield of oocytes and in vitro maturation in indigenous cow of Assam.

Keywords

Aspiration In vitro maturation Oocytes Slicing.

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