Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 49 issue 3 (june 2015) : 364-366

Evaluation of indirect Enzyme Linked Immuno Sorbent Assay and Virus Neutralization Test in the diagnosis of Bovine Herpes Virus – 1 (bhv-1) antibodies in cattle

M. Saravanajayam*, K. Kumanan, K.M. Palanivel
1Department of Veterinary Epidemiology and Preventive Medicine, TANUVAS, Madras Veterinary College, Chennai- 600 007, India.
Cite article:- Saravanajayam* M., Kumanan K., Palanivel K.M. (2024). Evaluation of indirect Enzyme Linked Immuno Sorbent Assay and Virus Neutralization Test in the diagnosis of Bovine Herpes Virus – 1 (bhv-1) antibodies in cattle . Indian Journal of Animal Research. 49(3): 364-366. doi: 10.5958/0976-0555.2015.00062.X.

ABSTRACT

To ascertain the prevalence of BHV-1 antibodies among cattle population, Virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA) were employed for the detection of BHV-1 antibodies. Evaluation of these two serological tests was made to find out the rapid, economic and sensitive diagnostic test for detection of BHV-1 infection. A total of 255 serum samples were collected from animals having respiratory and reproductive disorders for the detection of BHV-1 antibodies. All the serum samples were subjected to virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA). VNT and ELISA detected 127 (49.80 per cent) and 154 (60.39 per cent) cases of BHV-1 antibody positives respectively. The sensitivity, specificity of VNT in comparison with ELISA was 75.16 and 88.24 per cent respectively. In conclusion, ELISA is the rapid, reliable and technically superior test for the detection of BHV-1 antibodies.

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