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Current IssueEditorial BoardOnline First ArticlesArchive

volume 49 issue 3 (june 2015) : 364-366,   Doi: 10.5958/0976-0555.2015.00062.X

Evaluation of indirect Enzyme Linked Immuno Sorbent Assay and Virus Neutralization Test in the diagnosis of Bovine Herpes Virus – 1 (bhv-1) antibodies in cattle

M
M. Saravanajayam*
K
K. Kumanan
K
K.M. Palanivel
1Department of Veterinary Epidemiology and Preventive Medicine, TANUVAS, Madras Veterinary College, Chennai- 600 007, India.
Cite article:- Saravanajayam* M., Kumanan K., Palanivel K.M. (2025). Evaluation of indirect Enzyme Linked Immuno Sorbent Assay and Virus Neutralization Test in the diagnosis of Bovine Herpes Virus – 1 (bhv-1) antibodies in cattle . Indian Journal of Animal Research. 49(3): 364-366. doi: 10.5958/0976-0555.2015.00062.X.

ABSTRACT

To ascertain the prevalence of BHV-1 antibodies among cattle population, Virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA) were employed for the detection of BHV-1 antibodies. Evaluation of these two serological tests was made to find out the rapid, economic and sensitive diagnostic test for detection of BHV-1 infection. A total of 255 serum samples were collected from animals having respiratory and reproductive disorders for the detection of BHV-1 antibodies. All the serum samples were subjected to virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA). VNT and ELISA detected 127 (49.80 per cent) and 154 (60.39 per cent) cases of BHV-1 antibody positives respectively. The sensitivity, specificity of VNT in comparison with ELISA was 75.16 and 88.24 per cent respectively. In conclusion, ELISA is the rapid, reliable and technically superior test for the detection of BHV-1 antibodies.

REFERENCES

  1. Castrucci, G., Martin, W.B., Frigeri, F., Ferrari, M., Salvatori, D., Tagliati, S. and Cuteri, V., (1997). A serological survey of bovine herpes virus – 1 infection in selected dairy herds in northern and central Italy. Comp. Immun. Microbiol. Infect. Dis., 20(4): 315 - 317.
  2. Cho, H.J. and Bohac, J.G., (1985). Sensitivity and specificity of an enzyme linked immunosorbent assay for the detection of infectious bovine rhinotracheitis viral antibody in cattle. Can. J. Comp. Med., 49: 189 - 194.
  3. Dubuisson, J., Israel, B.A. and Letchworth III, G.C., (1992). Mechanisms of bovine herpes virus type 1 neutralization by monoclonal antibodies to glycoproteins gI, gIII and gIV. J. Gen. Virol., 73: 2031-2039.
  4. Edwards, S., Woods, S.B., Westcott, D.G., Emmerson, M., Jones, P.C. and Philips, A.J., (1986). An evaluation of five serological tests for the detection of antibody to bovine herpesvirus 1 in vaccinated and experimentally infected cattle. Res. Vet. Sci., 41: 378 - 382.
  5. Florent G. and Demarneffe, C.D., (1986). Enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses. Vet. Microbiol., 11: 309 - 317.
  6. Gibbs, E.P.J. and Rweyemamu, M.M., (1977). Bovine herpes viruses. Part I.Vet. Bull., 47(5): 317 – 343
  7. Kramps, J.A., Quak, S., Weerdmeester, K. and Van Oirschot, J.T., (1993). Comparative study on sixteen Enzyme - linked immunosorbent assay for the detection of antibodies to bovine herpes virus-1 in cattle. Vet. Microbiol., 35: 11 - 21.
  8. Miller, J.M., (1991). The effects of IBR virus infection on reproductive function of cattle. Vet. Med., 1: 95 - 98.
  9. Payment, P., Assaf, R.,Trudel, M. and Marois, P., (1979). Enzyme-linked immunosorbent assay for serology of infectious bovine rhino tracheitis virus infections. J. Clin. Microbiol.,10(5): 633 - 636.
  10. Philpott, M., (1993). The dangers of disease transmission by artificial insemination and embryo transfer. Br. Vet. J., 149: 339 - 343.
  11. Riegel, C.A., Ayers, V.K. and Collins, J.K., (1987). Rapid, sensitive, competitive serologic enzyme linked immunosorbent assay for detecting serum antibodies to bovine herpes virus type-1. J. Cli. Microbiol., 25(12): 2418 - 2421.
  12. Singh, B.K., Rama Kant. and Tongaonkar, S.S., (1983). Adaptation of Infectious bovine rhinotracheitis virus in Madin – Darby bovine kidney cell line and testing of buffalo sera for neutralizing antibodies. Indian J. Comp. Microbiol. Immunol. Infect. Dis., 4(1): 6 - 8.
  13. Snedecor, G.M. and Cochran, W.G., (1967). Statistical Methods, Fifth Edn. Iowa state Univ. Press. Ames. Iowa.
  14. Tongaonkar, S.S., Singh, B.K. and Rama Kant., (1986). Comparative prevalence of the infectious bovine rhinotracheitis, blue tongue, clamydia and brucella antibodies in dairy animals. Indian J. Comp. Microbiol. Immunol. Infect. Dis., 7(4): 139 - 143.
  15. Wyler, R., Engels, M. and Schwyzer, M., (1989). Infectious bovine rhinotracheitis / vulvovagititis (BHV-1). In: [Wittmann, G. (Ed.)], Herpes Virus Disease of Cattle, Horses and Pigs. Kluwer Academic Publishers, Boston, p.1.
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    volume 49 issue 3 (june 2015) : 364-366,   Doi: 10.5958/0976-0555.2015.00062.X

    Evaluation of indirect Enzyme Linked Immuno Sorbent Assay and Virus Neutralization Test in the diagnosis of Bovine Herpes Virus – 1 (bhv-1) antibodies in cattle

    M
    M. Saravanajayam*
    K
    K. Kumanan
    K
    K.M. Palanivel
    1Department of Veterinary Epidemiology and Preventive Medicine, TANUVAS, Madras Veterinary College, Chennai- 600 007, India.
    Cite article:- Saravanajayam* M., Kumanan K., Palanivel K.M. (2025). Evaluation of indirect Enzyme Linked Immuno Sorbent Assay and Virus Neutralization Test in the diagnosis of Bovine Herpes Virus – 1 (bhv-1) antibodies in cattle . Indian Journal of Animal Research. 49(3): 364-366. doi: 10.5958/0976-0555.2015.00062.X.

    ABSTRACT

    To ascertain the prevalence of BHV-1 antibodies among cattle population, Virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA) were employed for the detection of BHV-1 antibodies. Evaluation of these two serological tests was made to find out the rapid, economic and sensitive diagnostic test for detection of BHV-1 infection. A total of 255 serum samples were collected from animals having respiratory and reproductive disorders for the detection of BHV-1 antibodies. All the serum samples were subjected to virus neutralization test (VNT) and indirect Enzyme linked immunosorbent assay (ELISA). VNT and ELISA detected 127 (49.80 per cent) and 154 (60.39 per cent) cases of BHV-1 antibody positives respectively. The sensitivity, specificity of VNT in comparison with ELISA was 75.16 and 88.24 per cent respectively. In conclusion, ELISA is the rapid, reliable and technically superior test for the detection of BHV-1 antibodies.

    REFERENCES

    1. Castrucci, G., Martin, W.B., Frigeri, F., Ferrari, M., Salvatori, D., Tagliati, S. and Cuteri, V., (1997). A serological survey of bovine herpes virus – 1 infection in selected dairy herds in northern and central Italy. Comp. Immun. Microbiol. Infect. Dis., 20(4): 315 - 317.
    2. Cho, H.J. and Bohac, J.G., (1985). Sensitivity and specificity of an enzyme linked immunosorbent assay for the detection of infectious bovine rhinotracheitis viral antibody in cattle. Can. J. Comp. Med., 49: 189 - 194.
    3. Dubuisson, J., Israel, B.A. and Letchworth III, G.C., (1992). Mechanisms of bovine herpes virus type 1 neutralization by monoclonal antibodies to glycoproteins gI, gIII and gIV. J. Gen. Virol., 73: 2031-2039.
    4. Edwards, S., Woods, S.B., Westcott, D.G., Emmerson, M., Jones, P.C. and Philips, A.J., (1986). An evaluation of five serological tests for the detection of antibody to bovine herpesvirus 1 in vaccinated and experimentally infected cattle. Res. Vet. Sci., 41: 378 - 382.
    5. Florent G. and Demarneffe, C.D., (1986). Enzyme linked immunosorbent assay used to monitor serum antibodies to bovine respiratory disease viruses. Vet. Microbiol., 11: 309 - 317.
    6. Gibbs, E.P.J. and Rweyemamu, M.M., (1977). Bovine herpes viruses. Part I.Vet. Bull., 47(5): 317 – 343
    7. Kramps, J.A., Quak, S., Weerdmeester, K. and Van Oirschot, J.T., (1993). Comparative study on sixteen Enzyme - linked immunosorbent assay for the detection of antibodies to bovine herpes virus-1 in cattle. Vet. Microbiol., 35: 11 - 21.
    8. Miller, J.M., (1991). The effects of IBR virus infection on reproductive function of cattle. Vet. Med., 1: 95 - 98.
    9. Payment, P., Assaf, R.,Trudel, M. and Marois, P., (1979). Enzyme-linked immunosorbent assay for serology of infectious bovine rhino tracheitis virus infections. J. Clin. Microbiol.,10(5): 633 - 636.
    10. Philpott, M., (1993). The dangers of disease transmission by artificial insemination and embryo transfer. Br. Vet. J., 149: 339 - 343.
    11. Riegel, C.A., Ayers, V.K. and Collins, J.K., (1987). Rapid, sensitive, competitive serologic enzyme linked immunosorbent assay for detecting serum antibodies to bovine herpes virus type-1. J. Cli. Microbiol., 25(12): 2418 - 2421.
    12. Singh, B.K., Rama Kant. and Tongaonkar, S.S., (1983). Adaptation of Infectious bovine rhinotracheitis virus in Madin – Darby bovine kidney cell line and testing of buffalo sera for neutralizing antibodies. Indian J. Comp. Microbiol. Immunol. Infect. Dis., 4(1): 6 - 8.
    13. Snedecor, G.M. and Cochran, W.G., (1967). Statistical Methods, Fifth Edn. Iowa state Univ. Press. Ames. Iowa.
    14. Tongaonkar, S.S., Singh, B.K. and Rama Kant., (1986). Comparative prevalence of the infectious bovine rhinotracheitis, blue tongue, clamydia and brucella antibodies in dairy animals. Indian J. Comp. Microbiol. Immunol. Infect. Dis., 7(4): 139 - 143.
    15. Wyler, R., Engels, M. and Schwyzer, M., (1989). Infectious bovine rhinotracheitis / vulvovagititis (BHV-1). In: [Wittmann, G. (Ed.)], Herpes Virus Disease of Cattle, Horses and Pigs. Kluwer Academic Publishers, Boston, p.1.
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