ANTEMORTEM DIAGNOSIS OF RABIES FROM SKIN BY TaqMan REAL TIME PCR

DOI: 10.5958/0976-0555.2014.00038.7    | Article Id: B-2479 | Page : 597-600
Citation :- ANTEMORTEM DIAGNOSIS OF RABIES FROM SKIN BY TaqMan REAL TIME PCR.Indian Journal Of Animal Research.2014.(48):597-600
K. Bansal, C.K. Singh*, B.S. Sandhu, N.K. Sood and M. Dandale rabiesck@gmail.com
Address : Guru Angad Dev Veterinary and Animal Sciences University Ludhiana-141 001 India

Abstract

Present study revealed application of TaqMan real time PCR technique for diagnosis of rabies viral RNA from 11/20 antemortem skin samples with a sensitivity of 92.85%. The successful use of TaqMan real time PCR concluded that it was a viable, alternative tool for the antemortem diagnosis of rabies. Results correlated well with those of the post-mortem fluorescent antibody test performed on brain specimens of same animals.

Keywords

Antemortem diagnosis Rabies Skin TaqMan real time PCR

References

  1. Dacheux L, Reynes J M, Buchy P, Sivuth O, Diop B M, Rousset D, Rathat C, Jolly N, Dufourcq J B, Nareth C, Diop S, Rajerison I C R, Sadorge C and Bourhy H. (2008). A Reliable diagnosis of human rabies based on analysis of skin biopsy specimens. Clin. Infect. Dis., 47: 1410–1417.
  2. Emmons R W. (1979). Rabies diagnosis and rabies vaccine. N. Engl. J. Med., 301: 331–332.
  3. Fishbein D B, Smith J S, Rupprecht C, Clark K. (1991).Unexplained rabies in three immigrants in the United States. A virologic investigation. N. Engl. J. Med., 324: 205-11.
  4. Hanlon A C, Smith S J and Anderson R G. (1999). Article II: Laboratory diagnosis of rabies. J. Am. Vet. Med. Assoc., 215:1444-1447.Hughes G J, Smith J S, Hanlon C A and Rupprecht C E. (2004). Evaluation of a TaqMan PCR assay to detect rabies virus RNA: Influence of sequence variation and application to quantification of viral loads. J. Clin. Microbiol., 42(1): 299–306.
  5. Kaw A, Singh C K, Ramneek V, Sood N K, Sandhu B S, Deka D, Awahan S. (2012). Molecular detection of rabies by nested RT-PCR assay. Indian J. Anim. Sci., 81(4): 367-369.
  6. Macedo C I, Carnieli Jr P, Brandao P E, Travassos da Rosa E S, Oliveira Rde N, Castilho J G, Medeiros R, Machado R R, Oliveira R C, Carrieri M L and Kotait I. (2006). Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples. Braz. J. Infect. Dis., 10(5): 341-345.
  7. Madhusudana S N and Sukumaran S M. (2008). Antemortem diagnosis and prevention of human rabies. Ann. Indian Acad. Neurol., 11(1): 3–12.
  8. Menezes R. (2008). Rabies in India. Can. Med. Assoc. J., 178(5): 564-566.
  9. Nagaraj T, Joel P V, Desai A, Kamat A, Madhusudana S N and Ravi V. (2006). Ante mortem diagnosis of human rabies using saliva samples: Comparison of real time and conventional RT-PCR techniques. J. Clin. Microbiol., 44 (9): 3218–3224.
  10. Noah D L, Drenzek C L, Smith J S, Krebs J W, Orciari L, Shaddock J, Sanderlin D, Whitfield S, Fekadu M, Olson J G, Rupprecht C E and Childs J E. (1998). The epidemiology of human rabies in the United States, 1980–1996. Ann. Inter. Med., 11: 922–930.
  11. Strauss R, Granz A, Wassermann-Neuhold M, Krause R, Bago Z, Revilla-Fernandez S, Simon-Soria F, Echevarria J E, Popow-Kraupp T, Allerberger F, Schonbauer M and Hrabcik H. (2005). A human case of travel-related rabies in Austria, September 2004. Eurosurveillance. 10(11): 10-12.
  12. Wunner W H, Larson J K, Dietzschold B, and Smith C L. (1988). The molecular biology of rabies viruses. Rev. Infect. Dis., 10: 771-784.

Global Footprints