Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 48 issue 6 (december 2014) : 597-600

ANTEMORTEM DIAGNOSIS OF RABIES FROM SKIN BY TaqMan REAL TIME PCR

K. Bansal, C.K. Singh*, B.S. Sandhu, N.K. Sood, M. Dandale
1Guru Angad Dev Veterinary and Animal Sciences University Ludhiana-141 001 India
Cite article:- Bansal K., Singh* C.K., Sandhu B.S., Sood N.K., Dandale M. (2024). ANTEMORTEM DIAGNOSIS OF RABIES FROM SKIN BY TaqMan REAL TIME PCR. Indian Journal of Animal Research. 48(6): 597-600. doi: 10.5958/0976-0555.2014.00038.7.
Present study revealed application of TaqMan real time PCR technique for diagnosis of rabies viral RNA from 11/20 antemortem skin samples with a sensitivity of 92.85%. The successful use of TaqMan real time PCR concluded that it was a viable, alternative tool for the antemortem diagnosis of rabies. Results correlated well with those of the post-mortem fluorescent antibody test performed on brain specimens of same animals.
  1. Dacheux L, Reynes J M, Buchy P, Sivuth O, Diop B M, Rousset D, Rathat C, Jolly N, Dufourcq J B, Nareth C, Diop S, Rajerison I C R, Sadorge C and Bourhy H. (2008). A Reliable diagnosis of human rabies based on analysis of skin biopsy specimens. Clin. Infect. Dis., 47: 1410–1417.
  2. Emmons R W. (1979). Rabies diagnosis and rabies vaccine. N. Engl. J. Med., 301: 331–332.
  3. Fishbein D B, Smith J S, Rupprecht C, Clark K. (1991).Unexplained rabies in three immigrants in the United States. A virologic investigation. N. Engl. J. Med., 324: 205-11.
  4. Hanlon A C, Smith S J and Anderson R G. (1999). Article II: Laboratory diagnosis of rabies. J. Am. Vet. Med. Assoc., 215:1444-1447.Hughes G J, Smith J S, Hanlon C A and Rupprecht C E. (2004). Evaluation of a TaqMan PCR assay to detect rabies virus RNA: Influence of sequence variation and application to quantification of viral loads. J. Clin. Microbiol., 42(1): 299–306.
  5. Kaw A, Singh C K, Ramneek V, Sood N K, Sandhu B S, Deka D, Awahan S. (2012). Molecular detection of rabies by nested RT-PCR assay. Indian J. Anim. Sci., 81(4): 367-369.
  6. Macedo C I, Carnieli Jr P, Brandao P E, Travassos da Rosa E S, Oliveira Rde N, Castilho J G, Medeiros R, Machado R R, Oliveira R C, Carrieri M L and Kotait I. (2006). Diagnosis of human rabies cases by polymerase chain reaction of neck-skin samples. Braz. J. Infect. Dis., 10(5): 341-345.
  7. Madhusudana S N and Sukumaran S M. (2008). Antemortem diagnosis and prevention of human rabies. Ann. Indian Acad. Neurol., 11(1): 3–12.
  8. Menezes R. (2008). Rabies in India. Can. Med. Assoc. J., 178(5): 564-566.
  9. Nagaraj T, Joel P V, Desai A, Kamat A, Madhusudana S N and Ravi V. (2006). Ante mortem diagnosis of human rabies using saliva samples: Comparison of real time and conventional RT-PCR techniques. J. Clin. Microbiol., 44 (9): 3218–3224.
  10. Noah D L, Drenzek C L, Smith J S, Krebs J W, Orciari L, Shaddock J, Sanderlin D, Whitfield S, Fekadu M, Olson J G, Rupprecht C E and Childs J E. (1998). The epidemiology of human rabies in the United States, 1980–1996. Ann. Inter. Med., 11: 922–930.
  11. Strauss R, Granz A, Wassermann-Neuhold M, Krause R, Bago Z, Revilla-Fernandez S, Simon-Soria F, Echevarria J E, Popow-Kraupp T, Allerberger F, Schonbauer M and Hrabcik H. (2005). A human case of travel-related rabies in Austria, September 2004. Eurosurveillance. 10(11): 10-12.
  12. Wunner W H, Larson J K, Dietzschold B, and Smith C L. (1988). The molecular biology of rabies viruses. Rev. Infect. Dis., 10: 771-784.

Editorial Board

View all (0)