The present study was conducted to compare the effect of Lactic acid Bacillus and Yeast on the growth performance of Labrador pups. Twelve Labrador pups (3.5 months age, around 9.7 kg B.Wt) was assigned three dietary treatments of control diet T1 (Balanced Homemade diet), T2 (Control diet + Lactic acid bacillus) and T3 (Control diet + Yeast). The duration of experiment was 90 days. A digestibility trial was conducted at the mid of experiment. The results of digestibility studies revealed no significant difference on the digestibility of DM, OM, CP, CF, EE, NFE and NDF among treatment groups. The body weight gain was also statistically same in all the groups. All the blood parameters were within physiological limits and did not differ significantly except Total Protein level at 60 day in T2. The results conclusively indicated no significant effect of supplementing lactic acid bacteria and yeast on growth performance of pups under present experimental conditions.