Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 48 issue 5 (october 2014) : 418-421

GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS.

Sanjeev Singh*1, Sarita Kankoriya, Sudhir Kumar Jain, Hemlata Jain, Satyendra Pal Singh2
1Animal Biotechnology Centre, Madhya Pradesh Pashu Chikitsa Vigyan Vishwavidyalaya, Jabalpur- 482004, India
Cite article:- Singh*1 Sanjeev, Kankoriya Sarita, Jain Kumar Sudhir, Jain Hemlata, Singh2 Pal Satyendra (2024). GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS.. Indian Journal of Animal Research. 48(5): 418-421. doi: 10.5958/0976-0555.2014.00004.1.

ABSTRACT

15 microsatellite loci were investigated for studying the genetic polymorphism in Sahiwal (30) and Tharparker (30) breeds of cattle. Polymerase chain reactions (PCR) were carried out and amplified products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 15 microsatellite loci, 14 were found polymorphic. The number of alleles ranged from 3 to 7.  The overall polymorphic information content (PIC) values ranged from 0.293 to 0.775. Wright’s F statistics was carried out to determine the between breed (Fst) and within breed (Fis) genetic divergence. Fst value of 0.128 (P

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