GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS.

DOI: 10.5958/0976-0555.2014.00004.1    | Article Id: B-2430 | Page : 418-421
Citation :- GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS..Indian Journal Of Animal Research.2014.(48):418-421
Sanjeev Singh*1, Sarita Kankoriya, Sudhir Kumar Jain, Hemlata Jain and Satyendra Pal Singh2
Address : Animal Biotechnology Centre, Madhya Pradesh Pashu Chikitsa Vigyan Vishwavidyalaya, Jabalpur- 482004, India

Abstract

15 microsatellite loci were investigated for studying the genetic polymorphism in Sahiwal (30) and Tharparker (30) breeds of cattle. Polymerase chain reactions (PCR) were carried out and amplified products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 15 microsatellite loci, 14 were found polymorphic. The number of alleles ranged from 3 to 7.  The overall polymorphic information content (PIC) values ranged from 0.293 to 0.775. Wright’s F statistics was carried out to determine the between breed (Fst) and within breed (Fis) genetic divergence. Fst value of 0.128 (P

Keywords

Bos indicus Genetic diversity Microsatellite markers.

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