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volume 48 issue 5 (october 2014) : 418-421,   Doi: 10.5958/0976-0555.2014.00004.1

GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS.

S
Sanjeev Singh*1
S
Sarita Kankoriya
S
Sudhir Kumar Jain
H
Hemlata Jain
S
Satyendra Pal Singh2
1Animal Biotechnology Centre, Madhya Pradesh Pashu Chikitsa Vigyan Vishwavidyalaya, Jabalpur- 482004, India
Cite article:- Singh*1 Sanjeev, Kankoriya Sarita, Jain Kumar Sudhir, Jain Hemlata, Singh2 Pal Satyendra (2025). GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS.. Indian Journal of Animal Research. 48(5): 418-421. doi: 10.5958/0976-0555.2014.00004.1.

ABSTRACT

15 microsatellite loci were investigated for studying the genetic polymorphism in Sahiwal (30) and Tharparker (30) breeds of cattle. Polymerase chain reactions (PCR) were carried out and amplified products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 15 microsatellite loci, 14 were found polymorphic. The number of alleles ranged from 3 to 7.  The overall polymorphic information content (PIC) values ranged from 0.293 to 0.775. Wright’s F statistics was carried out to determine the between breed (Fst) and within breed (Fis) genetic divergence. Fst value of 0.128 (P

REFERENCES

  1. Botstein, D., White R L, Skolnick M and Davis R W (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am. J. Human Genet, 32: 324 – 331.
  2. Canon J., Alexandrino P., Bessa I., Carleos C., Carretero Y., Dunner S., Ferran N., Garcia D., Jordana J., Laloe D., Pereira A., Sanchez A. and Moazami-Goudarzi K. (2001). Genetic diversity measures of local European beef cattle breeds for conservation purposes. Genet. Sel. Evol. 33: 311-332.
  3. Kantannen, J. I., Olsaker L E, Holm S, Lien J, Vikki K, Brusgaard E, Eythorsdottir B Danell and Adalsteinsson S (2000).Genetic diversity and population structure of 20 North European cattle breeds. Ame. Genet. Ass. 91: 446-457.
  4. Metta, M., Kanginakudru S, Gudiseva N and Nagaraju J (2004). Genetic characterization of the Indian cattle breeds, Ongle and Deoni (Bo indicus), using microsatellite markers-a preliminary study BMC Genetics. 1:5.
  5. Mukesh, M., Sodhi M, Bhatia S and Mishra B P (2004). Genetic diversity of Indian cattle breeds as analysed with 20 microsatellite loci. J. Anim. Breeding Genet. 121: 416.
  6. Sambrook, J. and Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual. 3rd edi. Cold Spring Harbor Laboratory Press, New York.
  7. Schearf, B. (2003). World Watch List for Domestic Animal Diversity. 3rd edi. FAO, Rome, Italy.
  8. Sodhi M., Mukesh M, Prakash B, Ahalawati S P S and Sobti R C (2006). Microsatellite DNA typing for assessment of genetic variability in Tharparkar breed of Indian Zebu (Bos indicus) cattle, a major breed of Rajasthan. J. Genet, 85: 165-170.
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    volume 48 issue 5 (october 2014) : 418-421,   Doi: 10.5958/0976-0555.2014.00004.1

    GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS.

    S
    Sanjeev Singh*1
    S
    Sarita Kankoriya
    S
    Sudhir Kumar Jain
    H
    Hemlata Jain
    S
    Satyendra Pal Singh2
    1Animal Biotechnology Centre, Madhya Pradesh Pashu Chikitsa Vigyan Vishwavidyalaya, Jabalpur- 482004, India
    Cite article:- Singh*1 Sanjeev, Kankoriya Sarita, Jain Kumar Sudhir, Jain Hemlata, Singh2 Pal Satyendra (2025). GENETIC DIVERSITY ANALYSIS OF SAHIWAL AND THARPARKER BREEDS OF CATTLE (BOS INDICUS) BY MICROSATELLITE MARKERS.. Indian Journal of Animal Research. 48(5): 418-421. doi: 10.5958/0976-0555.2014.00004.1.

    ABSTRACT

    15 microsatellite loci were investigated for studying the genetic polymorphism in Sahiwal (30) and Tharparker (30) breeds of cattle. Polymerase chain reactions (PCR) were carried out and amplified products were resolved in 6% (denaturing) urea PAGE and stained with silver nitrate. Out of the 15 microsatellite loci, 14 were found polymorphic. The number of alleles ranged from 3 to 7.  The overall polymorphic information content (PIC) values ranged from 0.293 to 0.775. Wright’s F statistics was carried out to determine the between breed (Fst) and within breed (Fis) genetic divergence. Fst value of 0.128 (P

    REFERENCES

    1. Botstein, D., White R L, Skolnick M and Davis R W (1980). Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am. J. Human Genet, 32: 324 – 331.
    2. Canon J., Alexandrino P., Bessa I., Carleos C., Carretero Y., Dunner S., Ferran N., Garcia D., Jordana J., Laloe D., Pereira A., Sanchez A. and Moazami-Goudarzi K. (2001). Genetic diversity measures of local European beef cattle breeds for conservation purposes. Genet. Sel. Evol. 33: 311-332.
    3. Kantannen, J. I., Olsaker L E, Holm S, Lien J, Vikki K, Brusgaard E, Eythorsdottir B Danell and Adalsteinsson S (2000).Genetic diversity and population structure of 20 North European cattle breeds. Ame. Genet. Ass. 91: 446-457.
    4. Metta, M., Kanginakudru S, Gudiseva N and Nagaraju J (2004). Genetic characterization of the Indian cattle breeds, Ongle and Deoni (Bo indicus), using microsatellite markers-a preliminary study BMC Genetics. 1:5.
    5. Mukesh, M., Sodhi M, Bhatia S and Mishra B P (2004). Genetic diversity of Indian cattle breeds as analysed with 20 microsatellite loci. J. Anim. Breeding Genet. 121: 416.
    6. Sambrook, J. and Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual. 3rd edi. Cold Spring Harbor Laboratory Press, New York.
    7. Schearf, B. (2003). World Watch List for Domestic Animal Diversity. 3rd edi. FAO, Rome, Italy.
    8. Sodhi M., Mukesh M, Prakash B, Ahalawati S P S and Sobti R C (2006). Microsatellite DNA typing for assessment of genetic variability in Tharparkar breed of Indian Zebu (Bos indicus) cattle, a major breed of Rajasthan. J. Genet, 85: 165-170.
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