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Current IssueEditorial BoardOnline First ArticlesArchive

volume 45 issue 4 (december 2011) : 276 - 282

NUCLEOTIDE SEQUENCING AND PCR-SSCP OF MX1 GENE IN CHICKEN

C
Chinmoy Mishra*
D
Dharmeswar Das
P
Pushpendra Kumar
K
Kuldeep Khanna1
A
Ajit Pratap Singh
S
Shanker Dayal
S
Selvaramesh
T
Tarun Kumar Bhattacharya2
B
Bharat Bhushan
A
Arjava Sharma
1Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, India.

  • Submitted|

  • First Online |

  • doi

Cite article:- Mishra* Chinmoy, Das Dharmeswar, Kumar Pushpendra, Khanna1 Kuldeep, Singh Pratap Ajit, Dayal Shanker, Selvaramesh, Bhattacharya2 Kumar Tarun, Bhushan Bharat, Sharma Arjava (2025). NUCLEOTIDE SEQUENCING AND PCR-SSCP OF MX1 GENE IN CHICKEN. Indian Journal of Animal Research. 45(4): 276 - 282. doi: .

ABSTRACT

Mx1 is an interferon induced gene which inhibits the proliferation of single stranded RNA viruses. The size of Mx1 gene of chicken (Gallus gallus) is 21289 bp, having 14 exons and the coding sequence is 2118 bp long. Differential antiviral activity of Mx protein has been proved in various species including chicken. Polymerase Chain Reaction and subsequent Single Stranded Conformation Polymorphism (PCR-SSCP) analysis of 284 bp fragment of this gene was conducted to screen 153 birds of Aseel breed of chicken. Three genotypes viz. AB (0.74), BC (0.12) and AC (0.14) with three different alleles viz. A (0.44), B (0.43) and C (0.13) were identified. The homozygous genotypes i.e. AA, BB and CC could not be found in this population. Nucleotide sequencing of different alleles was done and submitted to the NCBI GenBank data (Acc. No.-GQ912704, GQ912705 and GQ912706). Nucleotide sequences were aligned with that of the corresponding Mx1 gene sequences of different breeds of chicken using DNASTAR Lasergene Software which revealed variations at five positions (91st, 107th, 113th, 194th and 231st). There is an urgent need of wide search for detection of polymorphism in Mx1 gene of different breeds of chicken and its possible association with the traits of economic importance.

REFERENCES

  1. Bassam, B.J., Caetano-Anolles, G. and Gresshoff, P. M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem., 196:80-83.
  2. Bernasconi, D., Schwartz, A. and Staeheli, P. (1993). The interferon-induced Mx protein of chickens lacks antiviral activity. J. Interferon Cytokine Res., 15:47-53.
  3. Falconer, D.S. and Mackay, T.F.C. (1996). Introduction to Quantitative Genetics. 4th ed. Addision Wesley Longmann Ltd. Essere. England, pp1-12.
  4. Ko, J.H., Jin, H. K., Asano, A., Takada, A., Ninomiya, A., Kida, H., Hokiyama, H., Ohara, M., Tsuzuki, M., Nishibori, M., Mizutani, M. and Watanabe. T. (2002). Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res., 12:595-601.
  5. Li, X.Y., Qu, L.J., Hou, Z.C., Yao, J.F., Xu, G.Y. and Yang, N. (2007). Genomic structure and diversity of the chicken Mx gene. Poult. Sci., 86:786-789.
  6. Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K. and Sekiya, T. (1989). Detection of polymorphisms of human DNA by gel electrophoresis as single strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA., 86:2766-2770.
  7. Sahoo, N.R. Pushpendra Kumar, Bhushan, B., Bhattacharya, T.K., Arjava Sharma, Dayal S., Kumar Pankaj P.and Sahoo, M. (2010). PCR-SSCP of serum lysozyme gene (Exon-III) in riverine buffalo and its association with lysozyme activity and somatic cell count. Asian-Aust. J. Anim. Sci., 23 (8):993-999.
  8. Sambrook, J. and Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual. 3rd Ed. Cold Spring Harbor Laboratory Press, New York, USA, pp 5.61-5.90.
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Indian Journal of Animal Research
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    volume 45 issue 4 (december 2011) : 276 - 282

    NUCLEOTIDE SEQUENCING AND PCR-SSCP OF MX1 GENE IN CHICKEN

    C
    Chinmoy Mishra*
    D
    Dharmeswar Das
    P
    Pushpendra Kumar
    K
    Kuldeep Khanna1
    A
    Ajit Pratap Singh
    S
    Shanker Dayal
    S
    Selvaramesh
    T
    Tarun Kumar Bhattacharya2
    B
    Bharat Bhushan
    A
    Arjava Sharma
    1Division of Animal Genetics, Indian Veterinary Research Institute, Izatnagar, Bareilly - 243 122, India.

    • Submitted|

    • First Online |

    • doi

    Cite article:- Mishra* Chinmoy, Das Dharmeswar, Kumar Pushpendra, Khanna1 Kuldeep, Singh Pratap Ajit, Dayal Shanker, Selvaramesh, Bhattacharya2 Kumar Tarun, Bhushan Bharat, Sharma Arjava (2025). NUCLEOTIDE SEQUENCING AND PCR-SSCP OF MX1 GENE IN CHICKEN. Indian Journal of Animal Research. 45(4): 276 - 282. doi: .

    ABSTRACT

    Mx1 is an interferon induced gene which inhibits the proliferation of single stranded RNA viruses. The size of Mx1 gene of chicken (Gallus gallus) is 21289 bp, having 14 exons and the coding sequence is 2118 bp long. Differential antiviral activity of Mx protein has been proved in various species including chicken. Polymerase Chain Reaction and subsequent Single Stranded Conformation Polymorphism (PCR-SSCP) analysis of 284 bp fragment of this gene was conducted to screen 153 birds of Aseel breed of chicken. Three genotypes viz. AB (0.74), BC (0.12) and AC (0.14) with three different alleles viz. A (0.44), B (0.43) and C (0.13) were identified. The homozygous genotypes i.e. AA, BB and CC could not be found in this population. Nucleotide sequencing of different alleles was done and submitted to the NCBI GenBank data (Acc. No.-GQ912704, GQ912705 and GQ912706). Nucleotide sequences were aligned with that of the corresponding Mx1 gene sequences of different breeds of chicken using DNASTAR Lasergene Software which revealed variations at five positions (91st, 107th, 113th, 194th and 231st). There is an urgent need of wide search for detection of polymorphism in Mx1 gene of different breeds of chicken and its possible association with the traits of economic importance.

    REFERENCES

    1. Bassam, B.J., Caetano-Anolles, G. and Gresshoff, P. M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Anal. Biochem., 196:80-83.
    2. Bernasconi, D., Schwartz, A. and Staeheli, P. (1993). The interferon-induced Mx protein of chickens lacks antiviral activity. J. Interferon Cytokine Res., 15:47-53.
    3. Falconer, D.S. and Mackay, T.F.C. (1996). Introduction to Quantitative Genetics. 4th ed. Addision Wesley Longmann Ltd. Essere. England, pp1-12.
    4. Ko, J.H., Jin, H. K., Asano, A., Takada, A., Ninomiya, A., Kida, H., Hokiyama, H., Ohara, M., Tsuzuki, M., Nishibori, M., Mizutani, M. and Watanabe. T. (2002). Polymorphisms and the differential antiviral activity of the chicken Mx gene. Genome Res., 12:595-601.
    5. Li, X.Y., Qu, L.J., Hou, Z.C., Yao, J.F., Xu, G.Y. and Yang, N. (2007). Genomic structure and diversity of the chicken Mx gene. Poult. Sci., 86:786-789.
    6. Orita, M., Iwahana, H., Kanazawa, H., Hayashi, K. and Sekiya, T. (1989). Detection of polymorphisms of human DNA by gel electrophoresis as single strand conformation polymorphisms. Proc. Natl. Acad. Sci. USA., 86:2766-2770.
    7. Sahoo, N.R. Pushpendra Kumar, Bhushan, B., Bhattacharya, T.K., Arjava Sharma, Dayal S., Kumar Pankaj P.and Sahoo, M. (2010). PCR-SSCP of serum lysozyme gene (Exon-III) in riverine buffalo and its association with lysozyme activity and somatic cell count. Asian-Aust. J. Anim. Sci., 23 (8):993-999.
    8. Sambrook, J. and Russell, D.W. (2001). Molecular Cloning: A Laboratory Manual. 3rd Ed. Cold Spring Harbor Laboratory Press, New York, USA, pp 5.61-5.90.
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