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volume 46 issue 2 (june 2012) : 107 - 113

DOUBLE MOLECULAR MARKERS-ASSISTED BREEDING OF SILKWORM, BOMBYX MORI, RESISTANT TO THE VIRUS, BMNPV AND BMDNV-Z

F
Feng Fan
S
Shi Haifeng
F
Fu Jingang
Q
Qin Lvgao
Z
Zhang Xiuwen
L
Liu Xiaoyong
C
Chen Keping
Y
Yao Qin*
1Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013ÿP. R. China

  • Submitted|

  • First Online |

  • doi

Cite article:- Fan Feng, Haifeng Shi, Jingang Fu, Lvgao Qin, Xiuwen Zhang, Xiaoyong Liu, Keping Chen, Qin* Yao (2025). DOUBLE MOLECULAR MARKERS-ASSISTED BREEDING OF SILKWORM, BOMBYX MORI, RESISTANT TO THE VIRUS, BMNPV AND BMDNV-Z. Indian Journal of Animal Research. 46(2): 107 - 113. doi: .

ABSTRACT

In order to improve the classic techniques of silkworm breeding and achieve the integration of good economic traits and disease resistance traits, a cross-breeding and molecular marker assisted breeding technology combined was developed in this experiment. The practical strain Jingsong and the strain NB, highly resistant to the BmNPV found from the library of silkworm races and strains in China, were selected as the breeding material. Through cross-breeding and backcrossing, the disease resistant gene was introducted into the economic strain Jingsong. At the same time, the strain Qiufeng, resistant to the BmDNV, was used as the donor material of resistance gene. In this experiment, a new strain resistant to the two virus, BmNPV and BmDNV-Z, was obtained through the method of virus administrion and molecular marker assisted breeding technology.

REFERENCES

  1. Bender W, Spierer P, Hogness DS (1983) Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. J Mol Biol 168:17-33.
  2. Chen K.P., Lin, C. Q., Yao, Q. (1996) Studies on the resistance to NPV and its heredity regularity in the silkworm (Bombyx mori L.). Acta Sericologica Sinica 22:160-164.
  3. Chen K.P., Lin, C. Q., Wu, D. X. (1991) Resistance of the conserved silkworm strains to nuclear polyhedrosis virus disease. Acta Sericologica Sinica 17:45-46.
  4. Eguchi R, Ninaki, O. and Hara, W. (1991) Genetical analysis on the nonsusceptibility to densonucleosis virus in the silkworm,Bombyx mori. J Seric Sci Jpn 60:384-389.
  5. Hayashiya K, Uchida, U. and Nishida, J., et al (1976) Comparison of anti-viral activities of the silkworm larvae reared in light and in darkness in relation to the formation of red fluorescent protein (RFP). J. Appl. Entomol. Zool 20:139-143.
  6. Ito K, Kidokoro K, Sezutsu H, Nohata J, Yamamoto K, Kobayashi I, Uchino K, Kalyebi A, Eguchi R, Hara W, Tamura T, Katsuma S, Shimada T, Mita K, Kadono-Okuda K (2008) Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus. Proc Natl Acad Sci U S A 105:7523-7.
  7. Kadono-Okuda K, E.Kosegawa,K.MaseandW.Hara (2002) Linkage analysis of maternal EST eDNA clones covering all twenty-eight chromosomes in the silkworm ,Bombyx mori. Insect Mol.Biol. 11:443-451
  8. Lu HS (1998) Molecular biology of insect viruses. China Agricultural Scientech Press., Beijing . p 1-15.
  9. Miao XX, Xub SJ, Li MH, Li MW, Huang JH, Dai FY, Marino SW, Mills DR, Zeng P, Mita K, Jia SH, Zhang Y, Liu WB, Xiang H, Guo QH, Xu AY, Kong XY, Lin HX, Shi YZ, Lu G, Zhang X, Huang W, Yasukochi Y, Sugasaki T, Shimada T, Nagaraju J, Xiang ZH, Wang SY, Goldsmith MR, Lu C, Zhao GP, Huang YP (2005) Simple sequence repeat-based consensus linkage map of Bombyx mori. Proc Natl Acad Sci U S A 102:16303-8.
  10. Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, Kanamori H, Namiki N, Kitagawa M, Yamashita H, Yasukochi Y, Kadono-Okuda K, Yamamoto K, Ajimura M, Ravikumar G, Shimomura M, Nagamura Y, Shin IT, Abe H, Shimada T, Morishita S, Sasaki T (2004) The genome sequence of silkworm, Bombyx mori. DNA Res 11:27-35.
  11. Meng ZQ (1982) Inheritance of silkworm’s resistance to nuclear polyhedrosis virus disease. Acta Sericologica Sinica 8:133-138.
  12. Nakazawa H, Tsuneishi E, Ponnuvel KM, Furukawa S, Asaoka A, Tanaka H, Ishibashi J, Yamakawa M (2004) Antiviral activity of a serine protease from the digestive juice of Bombyx mori larvae against nucleopolyhedrovirus. Virology 321:154-62.
  13. Promboon A, Shimada T., Fujisawa H. and Kobayashi M. (1995) Linkage map of random amplified polymorphic DNAs(RAPDs) in the silkworm, Bombyxmori. Genet.Res. 66:1-7.
  14. Ruchita S, Virendra,K.,et al. (2007) Identification of a Solube NADPH Oxidoreductase (BmNOX) with Antiviral Activity in the Gut Juice of Bombyx mori. Biochem. 71:200-205.
  15. Sturtevant AH (1915) No crossing over in the female of the silkworm moth. Am.Nat. 49:42-44.
  16. Tan YD, Wan C, Zhu Y, Lu C, Xiang Z, Deng HW (2001) An amplified fragment length polymorphism map of the silkworm. Genetics 157:1277-84.
  17. Tanaka Y, et al. (1913) A study of Mendelian factors in the silkworm ,Bombyx mori . J. Coll.Agric.TohokuImp.Univ. 5(4):91-113.
  18. Wang YJ, Yao Q, Chen KP, Wang Y, Lu J, Han X (2007) Characterization of the genome structure of Bombyx mori densovirus (China isolate). Virus Genes 35:103-8.
  19. Watanabe HaM, S. (1981) Genetically determined non-susceptibility of silkworm Bombyx mori to infection with a densonucleosis virus (Densovirus). J Invertebr Pathol 38:370-373.
  20. Xia Q, Zhou Z, Lu C, Cheng D, Dai F, Li B, Zhao P, Zha X, Cheng T, Chai C, Pan G, Xu J, Liu C, Lin Y, Qian J, Hou Y, Wu Z, Li G, Pan M, Li C, Shen Y, Lan X, Yuan L, Li T, Xu H, Yang G, Wan Y, Zhu Y, Yu M, Shen W, Wu D, Xiang Z, Yu J, Wang J, Li R, Shi J, Li H, Su J, Wang X, Zhang Z, Wu Q, Li J, Zhang Q, Wei N, Sun H, Dong L, Liu D, Zhao S, Zhao X, Meng Q, Lan F, Huang X, Li Y, Fang L, Li D, Sun Y, Yang Z, Huang Y, Xi Y, Qi Q, He D, Huang H, Zhang X, Wang Z, Li W, Cao Y, Yu Y, Yu H, Ye J, Chen H, Zhou Y, Liu B, Ji H, Li S, Ni P, Zhang J, Zhang Y, Zheng H, Mao B, Wang W, Ye C, Wong GK, Yang H (2004) A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science 306:1937-40.
  21. Q.Yao MLet al. (2003) Screening of molecular markers for NPV resistance in Bombyx mori L. [J].J.Appl.Ent. 127:134-136.
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Indian Journal of Animal Research
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    volume 46 issue 2 (june 2012) : 107 - 113

    DOUBLE MOLECULAR MARKERS-ASSISTED BREEDING OF SILKWORM, BOMBYX MORI, RESISTANT TO THE VIRUS, BMNPV AND BMDNV-Z

    F
    Feng Fan
    S
    Shi Haifeng
    F
    Fu Jingang
    Q
    Qin Lvgao
    Z
    Zhang Xiuwen
    L
    Liu Xiaoyong
    C
    Chen Keping
    Y
    Yao Qin*
    1Institute of Life Sciences, Jiangsu University, Zhenjiang, Jiangsu 212013ÿP. R. China

    • Submitted|

    • First Online |

    • doi

    Cite article:- Fan Feng, Haifeng Shi, Jingang Fu, Lvgao Qin, Xiuwen Zhang, Xiaoyong Liu, Keping Chen, Qin* Yao (2025). DOUBLE MOLECULAR MARKERS-ASSISTED BREEDING OF SILKWORM, BOMBYX MORI, RESISTANT TO THE VIRUS, BMNPV AND BMDNV-Z. Indian Journal of Animal Research. 46(2): 107 - 113. doi: .

    ABSTRACT

    In order to improve the classic techniques of silkworm breeding and achieve the integration of good economic traits and disease resistance traits, a cross-breeding and molecular marker assisted breeding technology combined was developed in this experiment. The practical strain Jingsong and the strain NB, highly resistant to the BmNPV found from the library of silkworm races and strains in China, were selected as the breeding material. Through cross-breeding and backcrossing, the disease resistant gene was introducted into the economic strain Jingsong. At the same time, the strain Qiufeng, resistant to the BmDNV, was used as the donor material of resistance gene. In this experiment, a new strain resistant to the two virus, BmNPV and BmDNV-Z, was obtained through the method of virus administrion and molecular marker assisted breeding technology.

    REFERENCES

    1. Bender W, Spierer P, Hogness DS (1983) Chromosomal walking and jumping to isolate DNA from the Ace and rosy loci and the bithorax complex in Drosophila melanogaster. J Mol Biol 168:17-33.
    2. Chen K.P., Lin, C. Q., Yao, Q. (1996) Studies on the resistance to NPV and its heredity regularity in the silkworm (Bombyx mori L.). Acta Sericologica Sinica 22:160-164.
    3. Chen K.P., Lin, C. Q., Wu, D. X. (1991) Resistance of the conserved silkworm strains to nuclear polyhedrosis virus disease. Acta Sericologica Sinica 17:45-46.
    4. Eguchi R, Ninaki, O. and Hara, W. (1991) Genetical analysis on the nonsusceptibility to densonucleosis virus in the silkworm,Bombyx mori. J Seric Sci Jpn 60:384-389.
    5. Hayashiya K, Uchida, U. and Nishida, J., et al (1976) Comparison of anti-viral activities of the silkworm larvae reared in light and in darkness in relation to the formation of red fluorescent protein (RFP). J. Appl. Entomol. Zool 20:139-143.
    6. Ito K, Kidokoro K, Sezutsu H, Nohata J, Yamamoto K, Kobayashi I, Uchino K, Kalyebi A, Eguchi R, Hara W, Tamura T, Katsuma S, Shimada T, Mita K, Kadono-Okuda K (2008) Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus. Proc Natl Acad Sci U S A 105:7523-7.
    7. Kadono-Okuda K, E.Kosegawa,K.MaseandW.Hara (2002) Linkage analysis of maternal EST eDNA clones covering all twenty-eight chromosomes in the silkworm ,Bombyx mori. Insect Mol.Biol. 11:443-451
    8. Lu HS (1998) Molecular biology of insect viruses. China Agricultural Scientech Press., Beijing . p 1-15.
    9. Miao XX, Xub SJ, Li MH, Li MW, Huang JH, Dai FY, Marino SW, Mills DR, Zeng P, Mita K, Jia SH, Zhang Y, Liu WB, Xiang H, Guo QH, Xu AY, Kong XY, Lin HX, Shi YZ, Lu G, Zhang X, Huang W, Yasukochi Y, Sugasaki T, Shimada T, Nagaraju J, Xiang ZH, Wang SY, Goldsmith MR, Lu C, Zhao GP, Huang YP (2005) Simple sequence repeat-based consensus linkage map of Bombyx mori. Proc Natl Acad Sci U S A 102:16303-8.
    10. Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, Kanamori H, Namiki N, Kitagawa M, Yamashita H, Yasukochi Y, Kadono-Okuda K, Yamamoto K, Ajimura M, Ravikumar G, Shimomura M, Nagamura Y, Shin IT, Abe H, Shimada T, Morishita S, Sasaki T (2004) The genome sequence of silkworm, Bombyx mori. DNA Res 11:27-35.
    11. Meng ZQ (1982) Inheritance of silkworm’s resistance to nuclear polyhedrosis virus disease. Acta Sericologica Sinica 8:133-138.
    12. Nakazawa H, Tsuneishi E, Ponnuvel KM, Furukawa S, Asaoka A, Tanaka H, Ishibashi J, Yamakawa M (2004) Antiviral activity of a serine protease from the digestive juice of Bombyx mori larvae against nucleopolyhedrovirus. Virology 321:154-62.
    13. Promboon A, Shimada T., Fujisawa H. and Kobayashi M. (1995) Linkage map of random amplified polymorphic DNAs(RAPDs) in the silkworm, Bombyxmori. Genet.Res. 66:1-7.
    14. Ruchita S, Virendra,K.,et al. (2007) Identification of a Solube NADPH Oxidoreductase (BmNOX) with Antiviral Activity in the Gut Juice of Bombyx mori. Biochem. 71:200-205.
    15. Sturtevant AH (1915) No crossing over in the female of the silkworm moth. Am.Nat. 49:42-44.
    16. Tan YD, Wan C, Zhu Y, Lu C, Xiang Z, Deng HW (2001) An amplified fragment length polymorphism map of the silkworm. Genetics 157:1277-84.
    17. Tanaka Y, et al. (1913) A study of Mendelian factors in the silkworm ,Bombyx mori . J. Coll.Agric.TohokuImp.Univ. 5(4):91-113.
    18. Wang YJ, Yao Q, Chen KP, Wang Y, Lu J, Han X (2007) Characterization of the genome structure of Bombyx mori densovirus (China isolate). Virus Genes 35:103-8.
    19. Watanabe HaM, S. (1981) Genetically determined non-susceptibility of silkworm Bombyx mori to infection with a densonucleosis virus (Densovirus). J Invertebr Pathol 38:370-373.
    20. Xia Q, Zhou Z, Lu C, Cheng D, Dai F, Li B, Zhao P, Zha X, Cheng T, Chai C, Pan G, Xu J, Liu C, Lin Y, Qian J, Hou Y, Wu Z, Li G, Pan M, Li C, Shen Y, Lan X, Yuan L, Li T, Xu H, Yang G, Wan Y, Zhu Y, Yu M, Shen W, Wu D, Xiang Z, Yu J, Wang J, Li R, Shi J, Li H, Su J, Wang X, Zhang Z, Wu Q, Li J, Zhang Q, Wei N, Sun H, Dong L, Liu D, Zhao S, Zhao X, Meng Q, Lan F, Huang X, Li Y, Fang L, Li D, Sun Y, Yang Z, Huang Y, Xi Y, Qi Q, He D, Huang H, Zhang X, Wang Z, Li W, Cao Y, Yu Y, Yu H, Ye J, Chen H, Zhou Y, Liu B, Ji H, Li S, Ni P, Zhang J, Zhang Y, Zheng H, Mao B, Wang W, Ye C, Wong GK, Yang H (2004) A draft sequence for the genome of the domesticated silkworm (Bombyx mori). Science 306:1937-40.
    21. Q.Yao MLet al. (2003) Screening of molecular markers for NPV resistance in Bombyx mori L. [J].J.Appl.Ent. 127:134-136.
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