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Indian Journal of Animal Research, volume 35 issue 2 (july to december 2001) : 132 - 134

OPTIMIZATION OF POLYMERASE CHAIN REACTION FOR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS

M.Parthiban*, T.p.Sujatha, V.Thiagarajan, R.Velmurugan

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¹Tamil Nadu Veterinary and Animal Sciences University, Chennai - 600 051, India

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Cite article:- M.Parthiban*, T.p.Sujatha, V.Thiagarajan, R.Velmurugan (2024). OPTIMIZATION OF POLYMERASE CHAIN REACTION FOR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS. Indian Journal of Animal Research. 35(2): 132 - 134. doi: .

ABSTRACT

Optimisation of reagents and conditions used in PCR has been carried out for detection of infectious bursal disease virus. Varying concentration of Taq DNA polymerase and dNTP mix does not much affect the PCR assay whereas varying concentration of MgCl2, IBD specific primers and annealing temperature has pronounced effect on the amplification reaction.

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REFERENCES

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OPTIMIZATION OF POLYMERASE CHAIN REACTION FOR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS

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