Chief Editor:

M. R. Saseendranath

Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

Frequency:Monthly

Indexing:

Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

DETECTION OF IBD VIRUS USING DOT ELISA WITH DIFFERENT BLOCKING AGENTS

M. Parthiban*, S. Manoharan, G. Ravikumar, N. Daniel Joy Chandran, G. Rajavelu

Affiliations

¹Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies, Madhavaram Milk Colony, Chennai - 600 051, India

Submitted|
First Online |
doi

Cite article:- Parthiban* M., Manoharan S., Ravikumar G., Chandran Joy Daniel N., Rajavelu G. (2025). DETECTION OF IBD VIRUS USING DOT ELISA WITH DIFFERENT BLOCKING AGENTS. Indian Journal of Animal Research. 37(2): 145 - 146. doi: .

ABSTRACT

The different blocking agents viz., BSA, gelatin, skim milk powder and acetic acid were used in dot-ELISA for detection of IBD virus from field samples to find out the sensitivity of the assay. Gelatin and BSA were equally sensitive and skim milk powder was found to be one fold increase in terms of sensitivity as well as intensity of colour reaction. Glacial acetic acid (diluted and undiluted) has produced only mild colour reaction with less sensitivity.

KEYWORDS

REFERENCES

Hawkes, R. etal (1982). Anal. Biochem., 119: 142.
Kumar, R. etaJ. (1996)./ndianJ Anim. Sci., 66: 994-997.
Maiti, N.K. etal (1993). Comp./mmunol Microbiol/nfect. Dis., 16: 245-250.
Marquardt, W. et al (1980). Avian Dis., 24: 375-385.
Natsukari, N. etal (1989). J; Immunol Math., 125: 156-166

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.