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M. R. Saseendranath

Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

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USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS

A. Uma, M. Parthiban, N. Daniel Joy Chandran, B. Thiyagarajan, P. Kalaimani Arasi, A. Koteeswaran

Affiliations

¹Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies. Madhavaram Milk Colony. Chennai - 600 051. India

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Cite article:- Uma A., Parthiban M., Chandran Joy Daniel N., Thiyagarajan B., Arasi Kalaimani P., Koteeswaran A. (2025). USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS. Indian Journal of Animal Research. 39(2): III - 114. doi: .

ABSTRACT

Monodon baculovirus (MBV) affects the cultured shrimp and results in production and economic
losses in the shrimp hatcheries and farms. Microscopic observation of the malachite green stained
wet mount squashes from the hepatopancreas for the presence of MBV occlusion bodies is the
widely followed method to diagnose MBV. This method was compared with a sensitive nested PCR
assay to grade the level of MBV infection in shrimp seeds (postlarvae). Out of 100 shrimp seed
samples screened for MBV, 20% of the seed samples were found to be positive for MBV by both
microscopic observation and I step PCR whereas 30% of the shrimp seeds samples were found to
be positive for MBV by II step or nested PCR only, The op'imum concentration of DNA to be used
for nested PCR assay for diagnosis of MBV was also standardized in this study.

KEYWORDS

REFERENCES

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