Chief EditorK.M.L. Pathak
Print ISSN 0367-6722
Online ISSN 0976-0555
NAAS Rating 6.50
SJR 0.263
Impact Factor 0.4 (2024)

Frequency :

Monthly (January, February, March, April, May, June, July, August, September, October, November and December)

Indexing Services :

Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus

Submit

Indian Journal of Animal Research, volume 39 issue 2 (july to december 2005) : III - 114

USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS

A. Uma, M. Parthiban, N. Daniel Joy Chandran, B. Thiyagarajan, P. Kalaimani Arasi, A. Koteeswaran

Affiliations

¹Vaccine Research Centre-Viral Vaccines, Centre for Animal Health Studies. Madhavaram Milk Colony. Chennai - 600 051. India

Submitted|
First Online |
doi

Cite article:- Uma A., Parthiban M., Chandran Joy Daniel N., Thiyagarajan B., Arasi Kalaimani P., Koteeswaran A. (2024). USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS. Indian Journal of Animal Research. 39(2): III - 114. doi: .

ABSTRACT

Monodon baculovirus (MBV) affects the cultured shrimp and results in production and economic
losses in the shrimp hatcheries and farms. Microscopic observation of the malachite green stained
wet mount squashes from the hepatopancreas for the presence of MBV occlusion bodies is the
widely followed method to diagnose MBV. This method was compared with a sensitive nested PCR
assay to grade the level of MBV infection in shrimp seeds (postlarvae). Out of 100 shrimp seed
samples screened for MBV, 20% of the seed samples were found to be positive for MBV by both
microscopic observation and I step PCR whereas 30% of the shrimp seeds samples were found to
be positive for MBV by II step or nested PCR only, The op'imum concentration of DNA to be used
for nested PCR assay for diagnosis of MBV was also standardized in this study.

KEYWORDS

REFERENCES

Chang, P.S. et aI. (1993). J. Invert. PathoI., 62: 116-120,
Flegel, T.w. et aI, (2001). Effects of hepatopancreatic parvovirus (HPV). Monodon baculovirus (MBV) and multiple viral infections on cultivated shrimp in Thailand symposium on sustainable shrimp aquaculture. Tokyo university of fisheries.
Hsu, YL. et aI. (2000), Dis. Aquat. Org,. 40: 93-99.
Li et aI. (2001). J. Fisheries China, 25: 141-146.
Lightner, DV (1996). A Hand Book of Shrimp Pathology and Diagnostic Procedures for Diseases of Cultural Penaeid Shrimp. World Aquaculture Society, Baton Rouge. LA. USA.
Poulos. B.J. et aI. (1994). J. Viral. Meth.. 49: 187-194.

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reviewed By

Share options

Editorial Board

View all (0)

USEFULNESS OF NESTED POLYMERASE CHAIN REACTION IN DETECTION OF MONODON BACULOVIRUS IN SHRIMP SEEDS

ABSTRACT

KEYWORDS

REFERENCES

Reviewed By

In this Article

APC

Publish With US

Become a Reviewer/Member

Open Access

Products and Services

Support and Policies

Editorial Board