volume 39 issue 1 (january to june 2005) : 63 '. 65

CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION

S
Satyajit Saha
N
Nandini
M
M.D. Venkatesha
R
R.N. ~inivasa Gowda
1ICAR Scheme on DNA based diagnostics for Leptospirosis. Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore . 560 024, India
  • Submitted|

  • First Online |

  • doi

Cite article:- Saha Satyajit, Nandini, Venkatesha M.D., Gowda ~inivasa R.N. (2025). CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION. Indian Journal of Animal Research. 39(1): 63 '. 65. doi: .
Gene fragment of Leptospira serovar Canicola was cloned in E. coli cells using pUC18
plasmid as a vector. The recombinant cells were selected on LB agar medium, which contained
ampicillin, X-gal and IPTG. From the recombinant white colonies, plasmid was extracted and
the leptospiral DNA insert was collected after digestion with EcoR I and used as probe in
hybridization experiments.
    1. Venlateha. M.D. and Ramadass, P. (1999). Indian J. Anim. Sci., 69: 942-944,
    2. Wagenaar. J et aJ. (2001). Am. J. Vet. Res.. 61: 316-320. 65
    volume 39 issue 1 (january to june 2005) : 63 '. 65

    CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION

    S
    Satyajit Saha
    N
    Nandini
    M
    M.D. Venkatesha
    R
    R.N. ~inivasa Gowda
    1ICAR Scheme on DNA based diagnostics for Leptospirosis. Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore . 560 024, India
    • Submitted|

    • First Online |

    • doi

    Cite article:- Saha Satyajit, Nandini, Venkatesha M.D., Gowda ~inivasa R.N. (2025). CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION. Indian Journal of Animal Research. 39(1): 63 '. 65. doi: .
    Gene fragment of Leptospira serovar Canicola was cloned in E. coli cells using pUC18
    plasmid as a vector. The recombinant cells were selected on LB agar medium, which contained
    ampicillin, X-gal and IPTG. From the recombinant white colonies, plasmid was extracted and
    the leptospiral DNA insert was collected after digestion with EcoR I and used as probe in
    hybridization experiments.
      1. Venlateha. M.D. and Ramadass, P. (1999). Indian J. Anim. Sci., 69: 942-944,
      2. Wagenaar. J et aJ. (2001). Am. J. Vet. Res.. 61: 316-320. 65
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      Indian Journal of Animal Research

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