Chief Editor:

M. R. Saseendranath

Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

Frequency:Monthly

Indexing:

Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION

Satyajit Saha, Nandini, M.D. Venkatesha, R.N. ~inivasa Gowda

Affiliations

¹ICAR Scheme on DNA based diagnostics for Leptospirosis. Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore . 560 024, India

Submitted|
First Online |
doi

Cite article:- Saha Satyajit, Nandini, Venkatesha M.D., Gowda ~inivasa R.N. (2025). CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION. Indian Journal of Animal Research. 39(1): 63 '. 65. doi: .

ABSTRACT

Gene fragment of Leptospira serovar Canicola was cloned in E. coli cells using pUC18
plasmid as a vector. The recombinant cells were selected on LB agar medium, which contained
ampicillin, X-gal and IPTG. From the recombinant white colonies, plasmid was extracted and
the leptospiral DNA insert was collected after digestion with EcoR I and used as probe in
hybridization experiments.

KEYWORDS

REFERENCES

Venlateha. M.D. and Ramadass, P. (1999). Indian J. Anim. Sci., 69: 942-944,
Wagenaar. J et aJ. (2001). Am. J. Vet. Res.. 61: 316-320. 65

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.