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Indian Journal of Animal Research, volume 39 issue 1 (january to june 2005) : 63 '. 65

CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION

Satyajit Saha, Nandini, M.D. Venkatesha, R.N. ~inivasa Gowda

Affiliations

¹ICAR Scheme on DNA based diagnostics for Leptospirosis. Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore . 560 024, India

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Cite article:- Saha Satyajit, Nandini, Venkatesha M.D., Gowda ~inivasa R.N. (2024). CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION. Indian Journal of Animal Research. 39(1): 63 '. 65. doi: .

ABSTRACT

Gene fragment of Leptospira serovar Canicola was cloned in E. coli cells using pUC18
plasmid as a vector. The recombinant cells were selected on LB agar medium, which contained
ampicillin, X-gal and IPTG. From the recombinant white colonies, plasmid was extracted and
the leptospiral DNA insert was collected after digestion with EcoR I and used as probe in
hybridization experiments.

KEYWORDS

REFERENCES

Venlateha. M.D. and Ramadass, P. (1999). Indian J. Anim. Sci., 69: 942-944,
Wagenaar. J et aJ. (2001). Am. J. Vet. Res.. 61: 316-320. 65

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CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION

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