CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION

Article Id: ARCC3298 | Page : 63 '. 65
Citation :- CLONING OF LEPTOSPIRA CAN/COLA SEROVAR GENE IN E. COU AND USING AS A PROBE IN SLOT BLOT HYBRIDIZATION.Indian Journal Of Animal Research.2005.(39):63 '. 65
Satyajit Saha, Nandini, M.D. Venkatesha and R.N. ~inivasa Gowda
Address : ICAR Scheme on DNA based diagnostics for Leptospirosis. Institute of Animal Health and Veterinary Biologicals, Hebbal, Bangalore . 560 024, India

Abstract

Gene fragment of Leptospira serovar Canicola was cloned in E. coli cells using pUC18
plasmid as a vector. The recombinant cells were selected on LB agar medium, which contained
ampicillin, X-gal and IPTG. From the recombinant white colonies, plasmid was extracted and
the leptospiral DNA insert was collected after digestion with EcoR I and used as probe in
hybridization experiments.

Keywords

References

  1. Venlateha. M.D. and Ramadass, P. (1999). Indian J. Anim. Sci., 69: 942-944,
  2. Wagenaar. J et aJ. (2001). Am. J. Vet. Res.. 61: 316-320. 65

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