Chief Editor:

M. R. Saseendranath

Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

Frequency:Monthly

Indexing:

Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

PCR BASED DETECTION OF ADHESIVE CURLI GENE “crl” AND ‘csgA’ IN AVIAN PATHOGENIC ESCHERICHIA COLI*

Manju Pal1, S.D. Singh2

Affiliations

¹Division of Avian Diseases, Indian Veterinary Research Institute, Izatnagar (U.P.) - 243 122, India

Submitted|
First Online |
doi

Cite article:- Pal1 Manju, Singh2 S.D. (2025). PCR BASED DETECTION OF ADHESIVE CURLI GENE “crl” AND ‘csgA’ IN AVIAN PATHOGENIC ESCHERICHIA COLI*. Indian Journal of Animal Research. 41(3): 226 - 229. doi: .

ABSTRACT

Twelve Escherichia coli isolates from colibacillosis cases of chickens were investigated for
the presence of genes encoding ‘curli adhesin (crl and csgA) by polymerase chain reaction using
two different sets of primers. The results showed the presence of crl gene in 7 isolates and csgA
gene in 5 isolates out of 12 isolates tested. Curli genes were ubiquitous among E.coli. Further,
Almost all the 12 isolates having curli genes were found to have haemagglutination (HA) ability
suggesting possible role of curli adhesin to mediate heamagglutination and enhancing pathogenecity process.

KEYWORDS

REFERENCES

Collinson, S.K. et al. (1991). J. Bacteriol., 173: 4773-4781.
Delicato, E.R. et al. (2003). Vet. Microbiol., 94: 97-103.
Dho-Moulin, M. and Fairbrother, J.M. (1999). Vet. Res., 30: 299-316.
Dozois, C.M. et al. (1992). Infection and Immunity, 60(7): 2648-2656.
Gross, W.G. (1994). In: Diseases Due to Escherichia in Poultry. (Gyles, C.L. ed). CAB International, Wallingford,
pp. 237-259.
Hulgren, S.J. et al. (1993). Cell, 73: 887-901.
Hulgren, S.J. et al. (1996). In: Escherichia coli and Salmonella typhimurium : Cellular and Molecular Biology.
(Neithardt, F.C. ed.), American Society for Microbiology, Washington D.C., pp. 2730-2756.
228 INDIAN JOURNAL OF ANIMAL RESEARCH
Knobl, T. et al. (2001). Vet. Microbio., 83: 71-80.
Maurer, J.J. et al. (1998). Avian Diseases, 42: 106-118.
Olsen, A. et al. (1989). Nature, 338-655.
Pourbakhsh, S.A. et al. (1997). Vet. Microbiol., 58: 195-213.
Provence, D.L. and Curtiss, R. (1992). Infection and Immunity, 60: 4460-4467.
Oyofo, B.A. et al. (1989). Poultry Sci., 68: 1351-1356.
Wooley, R.E. et al. (1992). Avian Dis., 36: 679-684.

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.