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Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

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SPERM NUCLEAR CHROMATIN DECONDENSATION TEST : ITS APPLICABILITY TO PREDICT FERTILITY OF CRYOPRESERVED SEMEN SAMPLES

C.S. Mukhopadhyay, Archana Verma, P.P. Dubey, Rupesh Jain, Nikhlesh Singh

Affiliations

¹Animal Genetics and Breeding Division, National Dairy Res. Institutes, Karnal-132 001, India

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Cite article:- Mukhopadhyay C.S., Verma Archana, Dubey P.P., Jain Rupesh, Singh Nikhlesh (2025). SPERM NUCLEAR CHROMATIN DECONDENSATION TEST : ITS APPLICABILITY TO PREDICT FERTILITY OF CRYOPRESERVED SEMEN SAMPLES. Indian Journal of Animal Research. 42(4): 285-287. doi: .

ABSTRACT

The nuclear chromatin decondensation test (NCDT) detects the ability of the sperm chromatin
to undergo decondensation following fertilization. The test does not have direct bearing with the
fertilizing ability of the spermatozoa but only reflects the proportion of sperm that may swell following
exposure to in vitro decondensing agents. The gross seminal parameters do not have any
direct relationship on the ability of the sperm chromatin to effect decondensation whether in vivo or
in vitro. The test can not be reliably used to assess the fertilizing potential of frozen thawed
semen of bovines.

KEYWORDS

REFERENCES

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