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M. R. Saseendranath

Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

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A COMPARATIVE STUDY BETWEEN MICROSCOPIC AGGLUTINATION TEST AND DIFFERENT PROTEIN ANTIGENS BASED ENZVME-LINKED IMMUNOSORBENT' ASSAY FOR THE DETECTION OF LEPTOSPIROSIS IN BOVINE

Surya Sankar, Hiron M. Harshan, Pallab Chaudhary, S. K. Srivastava

Affiliations

¹Division of Bacteriology and Mycology Indian Veterinary Research Institute, Izatnagar-243 122, India.

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Cite article:- Sankar Surya, Harshan M. Hiron, Chaudhary Pallab, Srivastava K. S. (2025). A COMPARATIVE STUDY BETWEEN MICROSCOPIC AGGLUTINATION TEST AND DIFFERENT PROTEIN ANTIGENS BASED ENZVME-LINKED IMMUNOSORBENT' ASSAY FOR THE DETECTION OF LEPTOSPIROSIS IN BOVINE. Indian Journal of Animal Research. 43(2): 111-113. doi: .

ABSTRACT

The present study compared the efficacy of Microscopic Agglutination Test (MAT) with UpL32
and UpL41 recombinant antigen based Enzyme-linked immunosorbent assay (ELISA) for the
diagnosis of bovine leptospirosis. The UpL32 and 41 genes were amplified using specific primers
and the polymerase chain reaction products (peR) were cloned. The gene inserts from positive
recombinant clones were inserted into expression vector and transformed in Escherichia coli DH5il
cells. The recombinant UpL32 and 41 proteins were expressed, purified and standardized. The
optimum antigen concentration of 100ng of each anti..;en was used for the screening of serum
samples from cattle suspected of leptospirosis. It was found that recombinant antigen ELISA detected
more positive cases of illness than MAT. Between the two recombinant antigens ELISA, UpL32 ELISA
was able to detect more positives.

KEYWORDS

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