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volume 47 issue 4 (august 2013) : 356-359

DEVELOPMENT AND EVALUATION OF A LATEX AGGLUTINATION TEST FOR THE DETECTION OF MYCOPLASMA GALLISEPTICUM ANTIBODIES IN CHICKEN SERA

S
Surya Sankar*
G
G. Krishnan Nair
M
M. Mini
M
M.Hiron Harshan
1Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur - 680 651, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Sankar* Surya, Nair Krishnan G., Mini M., Harshan M.Hiron (2025). DEVELOPMENT AND EVALUATION OF A LATEX AGGLUTINATION TEST FOR THE DETECTION OF MYCOPLASMA GALLISEPTICUM ANTIBODIES IN CHICKEN SERA. Indian Journal of Animal Research. 47(4): 356-359. doi: .

ABSTRACT

A rapid whole cell protein antigen (iron restricted Mycoplasma gallisepticum) based latex agglutination test (LAT) was developed to detect the presence of anti- Mycoplasma gallisepticum antibodies in the sera of poultry affected with mycoplasmosis. The test was found to be sensitive, specific and accurate in comparison with Haemagglutination Inhibition (HI) test. The rapidity, simplicity and economics of the LAT were found to fulfill the requirements of a screening test for Mycoplasma gallisepticum antibodies.

REFERENCES

  1. Carpenter, T.E., Mallinson, E.T., Miller, K.F., Gentry, R.F. and Schwartz, L.D. (1981). Vaccinations with F strain Mycoplasma gallisepticum to reduce production losses in layer chickens. Avian Dis., 25: 404–409.
  2. Kende, M. (1969). Antibody response of animals to Mycoplasma pneumoniae measured by latex agglutination. Appl. Microbiol., 17: 275-279.
  3. Madsen, M.L., Nettleton, D., Thacker, E.L. and Minion, F.C. (2006). Transcriptional profiling of Mycoplasma hyopneumoniae during iron depletion using microarrays. Microbiol, 152:937-944.
  4. Morton, H. E. (1966). Mycoplasma-Latex Agglutination Reaction J. Bacteriol., 92: 1196–1205.
  5. OIE (2008). Avian Mycoplasmosis (Mycoplasma gallisepticum, Mycoplasma synoviae). Terrestrial manual. Sixth edition. www.oie.int/Eng/normes/en_mmanual.htm.
  6. Ramadass, P., Samuel, B., Nachimuthu, K. (1999). A rapid agglutination test for the detection of leptospiral antibodies. Vet. Microbiol., 70: 137-140.
  7. Ramadass, P., Ananthi, R., Kumar, T.M.A and Ramaswamy, V. (2007). Rapid detection of Mycoplasma gallisepticum antibodies using latex agglutination test. Indian. Vet. J., 84: 127 – 129.
  8. Rurangirwa, F.R., Meguire, T. C. and Kibor, C. S. (1987). An inactivated vaccine for contagious caprine pleuropneumonia. Vet. Rec., 121: 397–402.
  9. Salyers, A. A. and Whitt, D. D. (2002). Bacterial Pathogeneis: a Molecular Approach, 2nd edition.
  10. Washington, DC: American Society for Microbiology.
  11. Zain, M. Z. and Bradbury, J. M. (1996). Optimising the conditions for isolation of Mycoplasma gallisepticum collected on applicator swabs. Vet. Microbiol., 49: 45–57.
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Indian Journal of Animal Research
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    volume 47 issue 4 (august 2013) : 356-359

    DEVELOPMENT AND EVALUATION OF A LATEX AGGLUTINATION TEST FOR THE DETECTION OF MYCOPLASMA GALLISEPTICUM ANTIBODIES IN CHICKEN SERA

    S
    Surya Sankar*
    G
    G. Krishnan Nair
    M
    M. Mini
    M
    M.Hiron Harshan
    1Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur - 680 651, India

    • Submitted|

    • First Online |

    • doi

    Cite article:- Sankar* Surya, Nair Krishnan G., Mini M., Harshan M.Hiron (2025). DEVELOPMENT AND EVALUATION OF A LATEX AGGLUTINATION TEST FOR THE DETECTION OF MYCOPLASMA GALLISEPTICUM ANTIBODIES IN CHICKEN SERA. Indian Journal of Animal Research. 47(4): 356-359. doi: .

    ABSTRACT

    A rapid whole cell protein antigen (iron restricted Mycoplasma gallisepticum) based latex agglutination test (LAT) was developed to detect the presence of anti- Mycoplasma gallisepticum antibodies in the sera of poultry affected with mycoplasmosis. The test was found to be sensitive, specific and accurate in comparison with Haemagglutination Inhibition (HI) test. The rapidity, simplicity and economics of the LAT were found to fulfill the requirements of a screening test for Mycoplasma gallisepticum antibodies.

    REFERENCES

    1. Carpenter, T.E., Mallinson, E.T., Miller, K.F., Gentry, R.F. and Schwartz, L.D. (1981). Vaccinations with F strain Mycoplasma gallisepticum to reduce production losses in layer chickens. Avian Dis., 25: 404–409.
    2. Kende, M. (1969). Antibody response of animals to Mycoplasma pneumoniae measured by latex agglutination. Appl. Microbiol., 17: 275-279.
    3. Madsen, M.L., Nettleton, D., Thacker, E.L. and Minion, F.C. (2006). Transcriptional profiling of Mycoplasma hyopneumoniae during iron depletion using microarrays. Microbiol, 152:937-944.
    4. Morton, H. E. (1966). Mycoplasma-Latex Agglutination Reaction J. Bacteriol., 92: 1196–1205.
    5. OIE (2008). Avian Mycoplasmosis (Mycoplasma gallisepticum, Mycoplasma synoviae). Terrestrial manual. Sixth edition. www.oie.int/Eng/normes/en_mmanual.htm.
    6. Ramadass, P., Samuel, B., Nachimuthu, K. (1999). A rapid agglutination test for the detection of leptospiral antibodies. Vet. Microbiol., 70: 137-140.
    7. Ramadass, P., Ananthi, R., Kumar, T.M.A and Ramaswamy, V. (2007). Rapid detection of Mycoplasma gallisepticum antibodies using latex agglutination test. Indian. Vet. J., 84: 127 – 129.
    8. Rurangirwa, F.R., Meguire, T. C. and Kibor, C. S. (1987). An inactivated vaccine for contagious caprine pleuropneumonia. Vet. Rec., 121: 397–402.
    9. Salyers, A. A. and Whitt, D. D. (2002). Bacterial Pathogeneis: a Molecular Approach, 2nd edition.
    10. Washington, DC: American Society for Microbiology.
    11. Zain, M. Z. and Bradbury, J. M. (1996). Optimising the conditions for isolation of Mycoplasma gallisepticum collected on applicator swabs. Vet. Microbiol., 49: 45–57.
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