Indian Journal of Animal Research

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Indian Journal of Animal Research, volume 43 issue 1 (march 2009) : 37-40

EVALUATION OF SEED GERMINATION TEST FOR EARLY DETECTION OF PREGNANCY IN COWS*

S.V. Rao Krishna1, T. Veena
1Department of Veterinary Physiology, Veterinary College, KVAFSU, Bangalore–560 024, India

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Cite article:- Krishna1 Rao S.V., Veena T. (2025). EVALUATION OF SEED GERMINATION TEST FOR EARLY DETECTION OF PREGNANCY IN COWS*. Indian Journal of Animal Research. 43(1): 37-40. doi: .

ABSTRACT

The urine samples collected from 40 crossbred cows/heifers during natural micturition early in the
morning on day 14, 21, 28, 35 and 45 after artificial insemination (AI) were subjected to seed
germination test using wheat seeds and green gram. A significant inhibition of seed germination
after 48 hours and shoot growth after four days in both types of seeds, discoloration of either seeds
or the germination fluid or both after 48 hours and deepening thereafter when compared to control
groups were taken as criteria for declaring positive for true pregnancy. The results indicated that
such observations could be noticed for the first time in 68 per cent of the test pregnant group,
especially 28 days post AI. But the efficacies for true pregnancy detection on days 35 and 45
were 100 per cent, each. This test may serve as a simple, non invasive, dependable, user friendly,
economical and door step technique for early pregnancy detection in cows, to the field veterinarians
and farmers equally.

REFERENCES

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  2. Shinde, A.D. (1998). M.V.Sc., dissertation, Maharashtra Agricultural University, Parbhani, India.
  3. Veena, T. and Narendranath, R. (1993). Curr. Sci. 65:989-990.
  4. Veena, T. et al. (1997). Adv. Contraceptives and Delivery Systems, 13:49-53.
  5. Wani, N. A. et al. (2003). Indian J. Dairy Sci. 56:1-8.
  6. Yadav, C.D. et al. (1999a). In: Proceedings of 86th Session of Indian Science Congress held at Chennai, 3rd -7th January, 1999.
  7. Yadav, C.D. et al. (1999b). In: Proceedings of 86th Session of Indian Science Congress held at Chennai, 3rd -7th January, 1999.
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