Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

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Indian Journal of Animal Research, volume 47 issue 3 (june 2013) : 240-243

DETECTION OF CD4/CD8 RATIO IN MICE IMMUNIZED WITH A BICISTRONIC PLASMID CONSTRUCT ENCODING A PPE GENE OF MYCOBACTERIUM AVIUM PARATUBERCULOSIS AND A CYTOKINE GENE OF MURINE GAMMA INTERFERON*

Rajib Deb, P. P. Goswami
1Division of Veterinary Biotechnology Indian Veterinary Research Institute, Izatnagar – 243 122, India

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Cite article:- Deb Rajib, Goswami P. P. (2024). DETECTION OF CD4/CD8 RATIO IN MICE IMMUNIZED WITH A BICISTRONIC PLASMID CONSTRUCT ENCODING A PPE GENE OF MYCOBACTERIUM AVIUM PARATUBERCULOSIS AND A CYTOKINE GENE OF MURINE GAMMA INTERFERON*. Indian Journal of Animal Research. 47(3): 240-243. doi: .

ABSTRACT

In the present study  a gene encoding PPE protein of M.a.paratuberculosis (FJ032182) was cloned with murine IFN-³  in a mammalian  bicistronic vector pIRES 6.1 to elucidate  the role of gamma interferon on the PPE gene for determination of CD4/CD8 ratio  in post immunized mice. Flow cytometric analysis with mononuclear mice splenocytes on 42nd day post immunization revealed significant reduction of the CD4/CD8 ratio in the mice group immunized with pIR PPE/IFN (1.65±0.0001) compared to the  pIR PPE group (2.92±0.0003). These result suggested that co-expression of murine IFNg in conjunction with PPE protein significantly enhanced the CMI response.

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