Chief Editor:

M. R. Saseendranath

Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA

Frequency:Monthly

Indexing:

Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

MOLECULAR CLONING OF A DNA FRAGMENT ENCODING 34.9 KDA CARBOXY TERMINAL PPE PROTEIN OF MYCOBACTERIUMAVIUMSUBSP. PARATUBERCULOSIS IN E.COLI *

R. Deb, P.P. Goswami, V.K. Saxena, N.S. Prasad

Affiliations

¹Department of Animal Biotechnology Indian Veterinary Research Institute. Izatnagar, 243122. India

Submitted|
First Online |
doi

Cite article:- Deb R., Goswami P.P., Saxena V.K., Prasad N.S. (2025). MOLECULAR CLONING OF A DNA FRAGMENT ENCODING 34.9 KDA CARBOXY TERMINAL PPE PROTEIN OF MYCOBACTERIUMAVIUMSUBSP. PARATUBERCULOSIS IN E.COLI *. Indian Journal of Animal Research. 44(2): 131 134. doi: .

ABSTRACT

Mycobacterium ar/ium ssp. Paratuberculosis (Map) is the causative agent of johne's disease in animals. A PPE (Proline Proline Glutamic acid rich) antigen encoding 34.9 kDa hypothetical protein of M avium subsp. ParatuberclIlosiswas cloned and sequenced. Sequence analysis revealed that no other published sequences having significant homology with that PPE gene .which elucidated that the PPE 34.9 kDa could be used as diagnostic antigen.

KEYWORDS

REFERENCES

Bannantine, J.P eta/. (2004). Am. Sodet. Microbjol .. 42: 106-114
Choudhary. R. K. S. eta/., (2003). Infect. Immun. 71:6338-6343.
Cole, S T eta/.. (1998).Nature393: 537-544.
Dillion. 0, C. etal.lnfect.Immun.(1999) 67:2941-2950
Fleischmann, R. D, eta!., (2002). J Bacteriol 184: 5479-5490.
Huntley. J.F. et al. (2005) Infect, Immun., 73: 6877-6884.
Mullerad, J. et a!., (2002a) FEMS Immunol. Med. Microbio!', 34: 81-88.
Mullerad, J. eta!., (2002b) Mo!. Microbio!' Immuno/.. 190: 179-187.
Mullerad, J. etal.. (2003) Microbiol. Pathogenesis, 34: 81-90.
Okkels, L M.. et aI., (2003). Infect. Immun. 71: 6116-6123.
Portillo, PD. eta/., (1991) Plasmid, 39: 100-113.
Sambrook, J. and Russel. D. W. (2001) Molecular cloning: A laboratory manual3,d edn. LoldSpring Harbor Laboratory Press. Cold spring Harbor;NY.

Disclaimer :

All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.

Copyright :

This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.