Banner
Current IssueEditorial BoardOnline First Articles
Current IssueEditorial BoardOnline First Articles
Indian Journal of
Animal Research
Print ISSN: 0367-6722
Online ISSN: 0976-0555
NASS Rating
6.40
SJR
0.233
CiteScore
0.606

Chief Editor:
M. R. Saseendranath
Kerala Veterinary and Animal Science University, Mannuthy, Thrissur, INDIA
Frequency:Monthly
Indexing:
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, ...

Indian Journal of Animal Research, volume 47 issue 2 (april 2013) : 147-151

DETERMINATION OF PRP LOCUS SNPS IN GAROLE SHEEP BREED FOR SCRAPIE

Sharda Choudhary1*, S.P. S Ahlawat, S.C. Gupta, Neelam Gupta
1Transgenic Research Laboratory, DNA Fingerprinting Unit, National Bureau of Animal Genetic Resources, Karnal-132 001, India

  • Submitted|

  • First Online |

  • doi

Cite article:- Choudhary1* Sharda, Ahlawat S S.P., Gupta S.C., Gupta Neelam (2025). DETERMINATION OF PRP LOCUS SNPS IN GAROLE SHEEP BREED FOR SCRAPIE. Indian Journal of Animal Research. 47(2): 147-151. doi: .

ABSTRACT

Experiment was carried out at National Bureau of Animal Genetic Resources, Karnal to identify polymorphisms of the prion protein gene (PrP) at the codons (136, 154 and 171) responsible for the susceptibility and resistance of the scrapie disease in the sheep. Blood samples of 50 animals of the Garole sheep breed were collected from its native tract in and around South 24 Pargana district of West Bengal from the eastern region. PCR amplicons of approximate size 358 bp were generated for PrP gene exon-3 partial CD’s covering 352 to 700 nucleotide positions. Genotype ARQ/ARQ was found in the analyzed population with the 100% frequency. SNP at codon 127 and 189 are GG/GS and QQ/QL with frequencies of 72.73/27.27 and 90.91/9.09 respectively. In all flocks, ARQ/ARQ genotype was the dominant haplotype regardless of sheep age, sex and health. However, the generated data will help for the detection of SNP markers related to disease resistance or susceptibility in India.

REFERENCES

  1. Bassam B J G, Catano A and Gresshoff P M. (1991). Fast and sensitive silver staining of DNA in polyacrylamide gels. Analytical Biochemistry 196:80-83.
  2. Baylis M and Goldmann W. (2004). The genetics of scrapie in sheep and goats. Current Molecular Medicine. 4: 385- 396.
  3. Choudhary S, Gupta N and Gupta S C. (2012). Identification of SNPs in PRNP gene in Karnah sheep breed of India. Indian Journal of Animal Sciences 82: 609–614.
  4. De Silva U, Guo X, Kufer D M, Fernando S C, Pillai A T V, Najar F Z, So S, Fitch G Q and Roe B A. (2003). Allelic variants of ovine prion protein gene (PRNP) in Oklahoma sheep. Cytogenetic and Genome Research. 102: 89- 94.
  5. DEFRA, website consultation, March 2002.
  6. Hunter N. (1996). Novel polymorphisms in the caprine PrP gene: a codon 142 mutation associated with scrapie incubation period. Journal of General Virology 77: 2885-2891.
  7. Hunter N, Goldmann, W., Smith G. and Hope J. (1994). The association of a codon 136 PrP gene variant with the occurrence of natural scrapie. Arch. Virol. 137: 171-177.
  8. Sambrook J, Fritsch E F and Maniatis T. (1989). In Molecular Cloning: A Laboratory Manual 2nd ed, Cold Spring Harbour, Cold Spring Laboratory Press, NY pp 121.
  9. Westaway D, DeArmond S, Cayetanocanlas J, Groth D, Foster D and Yang S. (1994). Degeneration of Skeletal-Muscle, Peripheral-Nerves, and the Central-Nervous-System in Transgenic Mice Overexpressing Wild-Type Prion Proteins. Cell 76: 117-129.
  10. Zhou H, Hickfford J G H, and Fang Q. (2005). Technical note: Determination of alleles of the ovine PRNP gene using PCR-Single strand conformation polymorphism analysis. Journal of Animal Science. 83: 745-749.
  11. Zlotnik I and Katiyar R D. (1961). The occurrence of scrapie disease in sheep of the remote Himalayan foothills. Veterinary Record. 73: 543-544.
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)