Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 40 issue 3 (september 2020) : 311-316,   Doi: 10.18805/ag.D-5086

Tricyclazole Induced Alterations in Certain Biomarker Enzymes of an Indian Paddy-field Fish, Channa punctatus (Bloch)

D
D.N. Pandit
U
U. Rani
S
S.K. Sharma
1Department of Zoology, Veer Kunwar Singh University, Arrah-802 301, Bihar, India. 
Cite article:- Pandit D.N., Rani U., Sharma S.K. (2020). Tricyclazole Induced Alterations in Certain Biomarker Enzymes of an Indian Paddy-field Fish, Channa punctatus (Bloch). Agricultural Science Digest. 40(3): 311-316. doi: 10.18805/ag.D-5086.

ABSTRACT

Tricyclazole, a systemic fungicide is recommended to treat of diseases in irrigated rice. Channa punctatus (Bloch) is a freshwater fish also found in paddy fields. This maiden study was designed to evaluate acute toxicity of tricyclazole and its responses of certain biomarker enzymes in Channa punctatus. By regression analysis method, 24, 48, 72 and 96hr-LC50 dose of tricyclazole was calculated 54.30, 36.76, 32.63 and 25.06 mg l-1 respectively. The range of LC50 dose indicates highly toxic nature of tricyclaozole. Fish were then exposed to 0.25 and 1.25 mg  l-1 sublethal dose of tricyclazole for short term (24, 48 and 96 hours) and long term (15, 30 and 45 days) exposure and the alterations of enzyme activities were determined. Alkaline phosphatase activities exposed to 0.25 mg l-1 were increased insignificantly (p>0.05) after 24 hours but increased significantly (p<0.05) to 0.25 and 1.25 mg l-1 tricyclazole at all other treatments. Alanine transaminase and aspartate transaminase increased significantly (p<0.05) in response to tricyclazole during both exposures. These findings may be used in the assessment of the potential risk of tricyclazole on food chain and aquatic ecosystems.

REFERENCES

  1. Adams, S.M., Ham, M.S., Greeley, M.S., LeHew, R.F., Hinton, D.E., Saylor, C.F. (1996). Downstream gradient in bioindicator responses: point source contaminant effects on fish health. Canadian Journal of Fisheries and Aquatic Science. 53: 2177-2187.
  2. Agius, C. and Coushman, W. (1986). Induction of enhanced alkaline phosphatase activity in the melanomacrophage centres of Oreochromis aureus (Steindachner) through starvation and vaccination. Journal of Fish Biology. 28: 87-92.
  3. Agrahari, S., Gopal, K., Pandey, K. (2006). Biomarkers of monocrotophos in a freshwater fish Channa punctatus (Bloch). Journal of Environmental Biology. 27: 453-457.
  4. Agrawal, M.K. and Sastry, K.V. (1979). HgCl2 induced enzymological changes in kidney and ovary of a teleost fish Channa punctatus. Bulletin of Environmental Contamination and Toxicology. 22(1-2): 38-43.
  5. Anwar, A. and Bhat, M.S. (2005). Efficacy of fungicides as seed treatment in the management of blast disease of rice in nursery bed. Agricultural Science Digest. (25):293-295.
  6. APHA (2005). Standard methods for the examination of water and waste water, 20th edition, Clesceri, L.S. Greenberg, A.E. and Eaton, A.D. (Eds.), American Public Health Association, Washington DC.
  7. Balasubaramanian, M.P., Dhandayuthapani, S., Neelaiappan, K., Amalingam, K. (1983). Comparative studies on phosphomonoesterase in helminthes. Helminthologia. 20: 111-120.
  8. Banaee, M., Sureda, A., Mirvaghefi, A.R., Ahmadi, K. (2011). Effects of diazinon on biochemical parameters of blood n rainbow trout (Oncorhynchus mykiss). Pesticide Biochemistry and Physiology. 99: 1-6.
  9. Durrieu, C. and Tran-Minh, C. (2002). Optical algal biosensor using alkaline phosphatase for determination of heavy metals. Ecotoxicology and Environmental Safety. 51(3): 206-209. 
  10. Edori, O.S, Dibofori-Orji, A.N., Edori, E.S. (2013). Biochemical changes in plasma and liver of Clarias gariepinus exposed to Paraquat. IOSR Journal of Pharmacy and Biological Sciences. 8(2): 35-39.
  11. Haider, Md.J. and Rauf, A. (2014). Sub-lethal effects of diazinon on hematological indices and blood biochemical parameters in Indian carp, Cirrhinus mrigala (Hamilton). Brazilian Archives of Biology and Technology. 57(6): 947-953.
  12. Loomis, T.A. and Hayes, A.W. (1996). Loomis’s essentials of toxicology. 4th ed., Cali­fornia, Academic press. pp. 208–245.
  13. Mazumder, N., Borah, S.K., Hatibarua, P. (2016). Management of leaf spot (Alternaria polianthi) of tuberose through fungicides. Agricultural Science Digest. 36: 250-252. DOI: 10.18805/    asd.v36i3.11454.
  14. Naik, G.R., Naik, G.B., Naik, B.T., Naika, K.R. (2012). Fungicidal management of leaf blast disease in rice. Global Journal of Bio-Science and Biotechnology. 1(1): 18-21.
  15. Padovani, L., Capri, E., Padovani, C., Puglisi, E., Trevisan, M. (2006). Monitoring tricyclazole residues in rice paddy watersheds. Chemosphere. 62: 303–314. 
  16. Pan, D.Y. and Liang, X.M. (1993). Safety study of pesticides on bog frog, a predatory natural enemy of pest in paddy field. Journal of Hunan Agricultural College. 19(1): 47-54. 
  17. Pandey, S., Kumar, R., Sharma, S., Nagpure, N.S., Srivastava, S.K. (2005). Acute toxicity bioassays of mercuric chloride and malathion on air breathing fish, Channa punctatus (Bloch). Ecotoxicology and Environmental Safety. 61: 114-120. 
  18. Qayoom, I., Balkhi, M.H., Shah, F.A., Bhat, B.A. (2019). Impairment induced due to oganophosphate compounds in some blood biochemical indices of fingerlings of Cyprinus carpio var. communis. Indian Journal of Animal Research. 53(10): 1326-1328. DOI: 10.18805/ijar.B-3662.
  19. Reish, D.L. and Oshida, O.S. (1987). Manual of Methods in aquatic environment research. Part 10. Short-term static bioassays. FAO Fisheries. Technical Paper No 247.
  20. Sancho, E. (2009). Physiological effects of tricyclazole on zebrafish (Danio rerio) and post-exposure recovery. Comparative Biochemistry and Physiology. 150: 25-32. 
  21. Shahsavani, D., Mohri, M., Kanani, H.G. (2010). Determination of normal values of some blood serum enzymes in Acipenser stellatus Pallas. Fish Physiology and Biochemistry. 36: 39–43.
  22. Shilpa, V., Indumathi, S.P., Radha, T. (2009) Ecological risk assesment of the fungicide tricyclazole (75%) on Ophiocephalus leucopunctatus (Sykes, 1839) with respect to hepatic enzymes and pathological anamolies. Research Journal of Agriculture and Biological Sciences. 5(4): 445-451.
  23. Splittstoesser, W.E., Chu, M.C., Stewart, S.A., Splittstoesser, S.A. (1976). Alanine aminotransferase from Cucurbita moschata cotyledons. Plant and Cell Physiology. 17(1): 83-89.
  24. Srivastava, A.S., Oohara, I., Suzuki, T., Shenouda, S., Singh, S.N., Chauhan, D.P. (2004). Purification and properties of cytosolic alanine aminotransferase from the liver of two freshwater fish, Clarias batrachus and Labeo rohita. Comparative Biochemistry and Physiology B Biochemistry and Molecular Biology. 137(2): 197-207.
  25. United State Environmental Protection Agency (USEPA) (2002). Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms EPA-821-R-02- 012. Washington D.C. (USA). U.S. Environmental Protection Agency Office of Water.
  26. United State Environmental Protection Agency (USEPA) (2013). Pesticide Ecotoxicity Database (Formerly: Environmental Effects Database (EEDB). Environmental Fate and Effects Division, USEPA. Washington, D.C.: EPA Office of Pesticides Program Database. 
Published In
Agricultural Science Digest
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 40 issue 3 (september 2020) : 311-316,   Doi: 10.18805/ag.D-5086

    Tricyclazole Induced Alterations in Certain Biomarker Enzymes of an Indian Paddy-field Fish, Channa punctatus (Bloch)

    D
    D.N. Pandit
    U
    U. Rani
    S
    S.K. Sharma
    1Department of Zoology, Veer Kunwar Singh University, Arrah-802 301, Bihar, India.&nbsp;
    Cite article:- Pandit D.N., Rani U., Sharma S.K. (2020). Tricyclazole Induced Alterations in Certain Biomarker Enzymes of an Indian Paddy-field Fish, Channa punctatus (Bloch). Agricultural Science Digest. 40(3): 311-316. doi: 10.18805/ag.D-5086.

    ABSTRACT

    Tricyclazole, a systemic fungicide is recommended to treat of diseases in irrigated rice. Channa punctatus (Bloch) is a freshwater fish also found in paddy fields. This maiden study was designed to evaluate acute toxicity of tricyclazole and its responses of certain biomarker enzymes in Channa punctatus. By regression analysis method, 24, 48, 72 and 96hr-LC50 dose of tricyclazole was calculated 54.30, 36.76, 32.63 and 25.06 mg l-1 respectively. The range of LC50 dose indicates highly toxic nature of tricyclaozole. Fish were then exposed to 0.25 and 1.25 mg  l-1 sublethal dose of tricyclazole for short term (24, 48 and 96 hours) and long term (15, 30 and 45 days) exposure and the alterations of enzyme activities were determined. Alkaline phosphatase activities exposed to 0.25 mg l-1 were increased insignificantly (p>0.05) after 24 hours but increased significantly (p<0.05) to 0.25 and 1.25 mg l-1 tricyclazole at all other treatments. Alanine transaminase and aspartate transaminase increased significantly (p<0.05) in response to tricyclazole during both exposures. These findings may be used in the assessment of the potential risk of tricyclazole on food chain and aquatic ecosystems.

    REFERENCES

    1. Adams, S.M., Ham, M.S., Greeley, M.S., LeHew, R.F., Hinton, D.E., Saylor, C.F. (1996). Downstream gradient in bioindicator responses: point source contaminant effects on fish health. Canadian Journal of Fisheries and Aquatic Science. 53: 2177-2187.
    2. Agius, C. and Coushman, W. (1986). Induction of enhanced alkaline phosphatase activity in the melanomacrophage centres of Oreochromis aureus (Steindachner) through starvation and vaccination. Journal of Fish Biology. 28: 87-92.
    3. Agrahari, S., Gopal, K., Pandey, K. (2006). Biomarkers of monocrotophos in a freshwater fish Channa punctatus (Bloch). Journal of Environmental Biology. 27: 453-457.
    4. Agrawal, M.K. and Sastry, K.V. (1979). HgCl2 induced enzymological changes in kidney and ovary of a teleost fish Channa punctatus. Bulletin of Environmental Contamination and Toxicology. 22(1-2): 38-43.
    5. Anwar, A. and Bhat, M.S. (2005). Efficacy of fungicides as seed treatment in the management of blast disease of rice in nursery bed. Agricultural Science Digest. (25):293-295.
    6. APHA (2005). Standard methods for the examination of water and waste water, 20th edition, Clesceri, L.S. Greenberg, A.E. and Eaton, A.D. (Eds.), American Public Health Association, Washington DC.
    7. Balasubaramanian, M.P., Dhandayuthapani, S., Neelaiappan, K., Amalingam, K. (1983). Comparative studies on phosphomonoesterase in helminthes. Helminthologia. 20: 111-120.
    8. Banaee, M., Sureda, A., Mirvaghefi, A.R., Ahmadi, K. (2011). Effects of diazinon on biochemical parameters of blood n rainbow trout (Oncorhynchus mykiss). Pesticide Biochemistry and Physiology. 99: 1-6.
    9. Durrieu, C. and Tran-Minh, C. (2002). Optical algal biosensor using alkaline phosphatase for determination of heavy metals. Ecotoxicology and Environmental Safety. 51(3): 206-209. 
    10. Edori, O.S, Dibofori-Orji, A.N., Edori, E.S. (2013). Biochemical changes in plasma and liver of Clarias gariepinus exposed to Paraquat. IOSR Journal of Pharmacy and Biological Sciences. 8(2): 35-39.
    11. Haider, Md.J. and Rauf, A. (2014). Sub-lethal effects of diazinon on hematological indices and blood biochemical parameters in Indian carp, Cirrhinus mrigala (Hamilton). Brazilian Archives of Biology and Technology. 57(6): 947-953.
    12. Loomis, T.A. and Hayes, A.W. (1996). Loomis’s essentials of toxicology. 4th ed., Cali­fornia, Academic press. pp. 208–245.
    13. Mazumder, N., Borah, S.K., Hatibarua, P. (2016). Management of leaf spot (Alternaria polianthi) of tuberose through fungicides. Agricultural Science Digest. 36: 250-252. DOI: 10.18805/    asd.v36i3.11454.
    14. Naik, G.R., Naik, G.B., Naik, B.T., Naika, K.R. (2012). Fungicidal management of leaf blast disease in rice. Global Journal of Bio-Science and Biotechnology. 1(1): 18-21.
    15. Padovani, L., Capri, E., Padovani, C., Puglisi, E., Trevisan, M. (2006). Monitoring tricyclazole residues in rice paddy watersheds. Chemosphere. 62: 303–314. 
    16. Pan, D.Y. and Liang, X.M. (1993). Safety study of pesticides on bog frog, a predatory natural enemy of pest in paddy field. Journal of Hunan Agricultural College. 19(1): 47-54. 
    17. Pandey, S., Kumar, R., Sharma, S., Nagpure, N.S., Srivastava, S.K. (2005). Acute toxicity bioassays of mercuric chloride and malathion on air breathing fish, Channa punctatus (Bloch). Ecotoxicology and Environmental Safety. 61: 114-120. 
    18. Qayoom, I., Balkhi, M.H., Shah, F.A., Bhat, B.A. (2019). Impairment induced due to oganophosphate compounds in some blood biochemical indices of fingerlings of Cyprinus carpio var. communis. Indian Journal of Animal Research. 53(10): 1326-1328. DOI: 10.18805/ijar.B-3662.
    19. Reish, D.L. and Oshida, O.S. (1987). Manual of Methods in aquatic environment research. Part 10. Short-term static bioassays. FAO Fisheries. Technical Paper No 247.
    20. Sancho, E. (2009). Physiological effects of tricyclazole on zebrafish (Danio rerio) and post-exposure recovery. Comparative Biochemistry and Physiology. 150: 25-32. 
    21. Shahsavani, D., Mohri, M., Kanani, H.G. (2010). Determination of normal values of some blood serum enzymes in Acipenser stellatus Pallas. Fish Physiology and Biochemistry. 36: 39–43.
    22. Shilpa, V., Indumathi, S.P., Radha, T. (2009) Ecological risk assesment of the fungicide tricyclazole (75%) on Ophiocephalus leucopunctatus (Sykes, 1839) with respect to hepatic enzymes and pathological anamolies. Research Journal of Agriculture and Biological Sciences. 5(4): 445-451.
    23. Splittstoesser, W.E., Chu, M.C., Stewart, S.A., Splittstoesser, S.A. (1976). Alanine aminotransferase from Cucurbita moschata cotyledons. Plant and Cell Physiology. 17(1): 83-89.
    24. Srivastava, A.S., Oohara, I., Suzuki, T., Shenouda, S., Singh, S.N., Chauhan, D.P. (2004). Purification and properties of cytosolic alanine aminotransferase from the liver of two freshwater fish, Clarias batrachus and Labeo rohita. Comparative Biochemistry and Physiology B Biochemistry and Molecular Biology. 137(2): 197-207.
    25. United State Environmental Protection Agency (USEPA) (2002). Methods for Measuring the Acute Toxicity of Effluents and Receiving Waters to Freshwater and Marine Organisms EPA-821-R-02- 012. Washington D.C. (USA). U.S. Environmental Protection Agency Office of Water.
    26. United State Environmental Protection Agency (USEPA) (2013). Pesticide Ecotoxicity Database (Formerly: Environmental Effects Database (EEDB). Environmental Fate and Effects Division, USEPA. Washington, D.C.: EPA Office of Pesticides Program Database. 
    In this Article
      Contact us
      Follow us
      Published In
      Agricultural Science Digest

      Editorial Board

      View all (0)