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Research Article

Agricultural Science Digest, volume 40 issue 2 (june 2020) : 115-121

Electrophoretic Evaluation of Major Seed Storage Protein Fraction, Gliadins and Glutenins of Eighty-Six Indian Wheat Genotypes

Monika Sihmar, Jitendra Kumar Sharma, Anita Rani Santal, N.P. Singh
1Centre for Biotechnology, M.D. University, Rohtak-124 001, Haryana, India. 
Cite article:- Sihmar Monika, Sharma Kumar Jitendra, Santal Rani Anita, Singh N.P. (2020). Electrophoretic Evaluation of Major Seed Storage Protein Fraction, Gliadins and Glutenins of Eighty-Six Indian Wheat Genotypes. Agricultural Science Digest. 40(2): 115-121. doi: 10.18805/ag.D-5085.

ABSTRACT

Wheat seed storage proteins, mainly gliadins and glutenins are the major proteins involved in the determination of bread-making quality. In this study, the variation in the polypeptide patterns of eighty-six Indian wheat genotypes and their interrelationships was studied using gliadins and glutenins fractions by using polyacrylamide gel electrophoresis. A substantial variation was recorded in gliadin fraction in the range of polypeptides of molecular weight range 40-45, 33-37, 29-32 kDa. The glutenins showed variation in the range of mol. wt. 75-110, 40-46, 22-36 kDa. Based on the polypeptide patterns of gliadin and glutenin fractions of eighty-six wheat lines, a dendrogram was constructed, which showed the genetic relationship among the different genotypes of wheat. 

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