Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 40 issue 3 (september 2020) : 260-264,   Doi: 10.18805/ag.D-5026

Ultra Structural Studies of the Ovaries of Susceptible and Insecticide-Resistant Plutella xylostella (Linnaeus)

A
Anureet Kaur Chandi
R
Rabinder Kaur
1Department of Entomology, Punjab Agricultural University, Ludhiana-141 004, Punjab, India.
Cite article:- Chandi Kaur Anureet, Kaur Rabinder (2020). Ultra Structural Studies of the Ovaries of Susceptible and Insecticide-Resistant Plutella xylostella (Linnaeus). Agricultural Science Digest. 40(3): 260-264. doi: 10.18805/ag.D-5026.

ABSTRACT

The present study was aimed to compare the ultra-structural changes in ovaries of susceptible and insecticide-resistant P. xylostella. Ovaries of the adults of P. xylostella from the susceptible and insecticide-resistant populations were dissected, prepared chemically and were observed under scanning electron microscope. The length and breadth of the ovarian follicles were measured and their size was calculated. Considerable variations in shape, size and appearance of ovarioles in susceptible and resistant moths were recorded. Ultra structural studies revealed reduction in dimensions i.e. length, breadth and consequent size of the ovarian follicle in resistant moths as compared to the susceptible one. The variations in the dimensions of ovarian follicles in resistant and susceptible moths are indicators of influence of insecticides there upon.

REFERENCES

  1. Aziza, E. and Abdel, A. (2012). Effect of three insect growth regulators (chlorfluazuron, tebofenozoid and pyriproxyfen) on fecundity of S. littoralis, histopathological and some biochemical aspects of moth ovary. Egyptian Academic Journal of Biological Sciences. 4: 49-59.
  2. Chandi, A.K. and Singh, G. (2013). Susceptibility status of diamondback moth, Plutella xylostella (Linnaeus) to commonly used insecticides. Journal of Insect Science. 26(2): 188-191.
  3. Chandi, R.S., Chandi A.K., Singh G. and Suri K.S. (2012). Insecticide use pattern on cole crops in Punjab. Journal of Insect Science. 25: 210-13.
  4. Chapman, J. W., Reynolds, D. R., Smith, A. D., Riley, J. R., Pedgley, D.E. and Woiwod, I. P. (2002). High altitude migration of the diamondback moth Plutella xylostella to the UK: A study using radar, aerial netting and ground trapping. Ecological Entomology. 27: 641-50.
  5. Chu, Y. (1986). The migration of the diamondback moth. In: Diamondback Moth Management: Proc 1st Int Wkshop. (Talekar, N.S and Greggs, T. D. eds.). Asian Vegetable Research and Development Centre, Shanhua, Taiwan. pp. 77-81.
  6. Coulson, S.J., Hodkinson, I.D., Webb, N.R., Mikkola, K., Harrison, J.A. and Pedgley, D.E. (2002). Aerial colonization of high Arctic islands by invertebrates: the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) as a potential indicator species. Diversity and Distriution. 8: 327-34.
  7. Fletcher, T.B. (1914). Some south Indian insects. Superintendent Government Press, Madras. 
  8. Gautam, M.P., Singh, H., Kumar, S., Kumar, V., Singh, G. and Singh, S.N. (2018). Diamondback moth, Plutella xylostella (Linnaeus) (Insecta: Lepidoptera: Plutellidae) a major insect of cabbage in India: A review. Journal of Entomology and Zoology Studies. 6(4): 1394-1399.
  9. Ghazawi, A.N., El-Shranoubi, E.D., El-Shazly, M.M. and Abdel Rahman, A. (2007). Effects of azadirachtin on mortality and reproductive system of the grasshopper, Heteracris littoralis Ramb. (Orthoptera : Acrididae). Journal of Orthoptera Research. 16(1): 57-65.
  10. Honda, K. (1990). Hibernation and migration of diamondback moth in northern Japan. In: Proc 2nd Int Wkshop (Talekar, N.S. and Greggs, T.D. eds.). Taiwan. pp. 43-50.
  11. Honda, K., Miyahara, Y. and Kegasawa, K. (1992). Seasonal abundance and the possibility of spring immigration of the diamondback moth Plutella xylostella (Linnaeus) (Lepidoptera: Yponomeutidae), in Morioka city, northern Japan. Appied Entomology and Zoology. 27: 517-25.
  12. Jallow, M.F.A and Hoy, C.W. (2007). Indirect selection for increased susceptibility to permethrin in diamondback moth (Lepidoptera: Plutellidae). Journal of Economic Entomology. 100(2): 526-33.
  13. Kaur, K. (2015). Sublethal effects of pyriproxyfen on tobacco caterpillar, Spodoptera litura (Fabricius). M.Sc Thesis. Punjab Agricultural University, Ludhina, India.
  14. Khaliq, A., Attique, M.N.R. and Sayyed, A. H. (2007). Evidence for resistance to pyrethroids and organophosphates in Plutella xylostella (Lepidoptera: Plutellidae) from Pakistan. Bulltein of Entomological Research. 97: 191-200.
  15. Lal, A. (1992). Studies on biology of diamondback moth, Plutella xylostella (Linn.) on cauliflower in Punjab. M.Sc. Thesis, Punjab Agricultural University, Ludhiana, India.
  16. Liang, G.M., Chen, W. and Liu, T.X. (2003). Effects of three neem-based insecticides on diamondback moth (Lepidoptera: Plutellidae). Crop Protection. 22: 333-40.
  17. Liu, S.S., Chen, F.Z. and Zalucki, M.P. (2002). Development and survival of the diamondback moth (Lepidoptera: Plutellidae) at constant and alternating temperatures. Environmenta Entomology. 31: 221-31.
  18. Loeb, M., Brandt, E.P. and Birnbaum, M.J. (1984). Ecdysteroid production by testes of budworm, Heliothis virescens from last larval instar to adult. Journal of Insect Physiology. 30: 375-81.
  19. Maiza, A., Kilani, S., Farine, J.P., Smagghe, G., Aribi, N. and Soltani, N. (2004). Reproductive effects in German cockroaches by ecdysteroid agonist RH-0345, juvenile hormone analogue methoprene and carbamate benfuracarb. Communication in Agriculture and Applied Biological Sciences. 69: 257–266.
  20. Meena, S. and Singh, N.P. (2014). Ultrastructural changes in female reproductive organ of Chrotogonus trachypterus Blanchard induced by deltamethrin. IOSR Journal of Agriculture and Veterinary Science. 7: 1-6.
  21. Mota-Sanchez, D., Bills, P.S. and Whalon, M.E. (2002). Arthropod resistance to pesticides: Status and overview. In: Pesticides in agriculture and the environment. (Wheeler, W.B. ed.). pp 241-272. Marcel Dekker Incorporation, New York.
  22. Moura, A.P., Carvalho, G.A. , Cosme, L.V., Alves, E., Botton, M. and Silva, P.S. (2011). Toxicological and ultrastructural analysis of the impact of pesticides used in temperate fruit crops on two populations of Chrysoperla externa (Neuroptera, Chrysopidae). Revista Brasileira de Entomologia. 55(3): 411–418.
  23. Nomura, M. and Miyata, T. (2000). Effects of pyriproxyfen, insect growth regulator on reproduction of Spodoptera litura, common cutworm. Japanese Journal of Applied Entomology and Zoology. 44: 81-88.
  24. Oliveira, A.C., Siqueira, H.A.A., Oliveira, J.V., Silva, J.E. and Filho, M.M. (2011). Resistance of Brazilian diamondback moth populations to insecticides. Scientia Agricola. 68(2):154-59. 
  25. Perlak, F.J., Stone, T.B., Muskipf, Y., Petersen, L.J., Parker, G.B., McPherson, S.A., Wyman, J., Love, S., Reed, G., Biever, D. and Fishhoff, D.A. (1993). Genetically improved potatoes: protection from damage by Colorado potato beetles. Plant Molecular Biology. 22: 313-21. 
  26. Perveen, F. and Miyata, T. (2000). Effects of sublethal dose of chlorfluazuron on ovarian development and oogenesis in the common cutworm Spodoptera litura (Lepidoptera: Noctuidae). Annals of Entomological Society of America. 93(5): 1131-37. 
  27. Santos, D.C.D. and Gregorio, E.A. (2002). Ultrastructure of the ovariole sheath in Diatraea saccharalis (Lepidoptera: Pyralidae). Biocell. 26: 229-35.
  28. Saxena, J.D., Rai, S., Srivastava, K.M. and Sinha, S.R. (1989). Resistance in the field populations of diamondback moth to some commonly used synthetic pyrethroids. Indian Journal of Entomology. 51: 265-268.
  29. Shelton, A.M. (2004). Management of diamondback moth: In: Proc 4th Int Wkshop, 26-29 November 2001. (Enderby, N.M. and Ridland, P.M. eds.). pp 38. Department of Natural Resources and Environment, Melbourne.
  30. Singh, K.R., Kaur, R., Chandi, A.K. and Singh, G. (2017). Influence of spinosad on reproduction of diamond backmoth Plutella xylostella (Linnaeus). Indian Journal of Entomology. 79(4): 467-473.
  31. Verkerk, R.H.J. and Wright, D.J. (1996). Multi-trophic interactions and management of the P. xylostella. A review. Bulletin of Entomological Research. 86: 205-216.
  32. Xu, Q., Tang, B., Zou, Q., Zheng, H., Liu, X. and Wang, S. (2015). Effects of pyriproxyfen on female reproduction in the common cutworm, Spodoptera litura (F.) (Lepidoptera: Noctuidae). PLoS ONE. 10(10): e0138171.
     
Published In
Agricultural Science Digest
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 40 issue 3 (september 2020) : 260-264,   Doi: 10.18805/ag.D-5026

    Ultra Structural Studies of the Ovaries of Susceptible and Insecticide-Resistant Plutella xylostella (Linnaeus)

    A
    Anureet Kaur Chandi
    R
    Rabinder Kaur
    1Department of Entomology, Punjab Agricultural University, Ludhiana-141 004, Punjab, India.
    Cite article:- Chandi Kaur Anureet, Kaur Rabinder (2020). Ultra Structural Studies of the Ovaries of Susceptible and Insecticide-Resistant Plutella xylostella (Linnaeus). Agricultural Science Digest. 40(3): 260-264. doi: 10.18805/ag.D-5026.

    ABSTRACT

    The present study was aimed to compare the ultra-structural changes in ovaries of susceptible and insecticide-resistant P. xylostella. Ovaries of the adults of P. xylostella from the susceptible and insecticide-resistant populations were dissected, prepared chemically and were observed under scanning electron microscope. The length and breadth of the ovarian follicles were measured and their size was calculated. Considerable variations in shape, size and appearance of ovarioles in susceptible and resistant moths were recorded. Ultra structural studies revealed reduction in dimensions i.e. length, breadth and consequent size of the ovarian follicle in resistant moths as compared to the susceptible one. The variations in the dimensions of ovarian follicles in resistant and susceptible moths are indicators of influence of insecticides there upon.

    REFERENCES

    1. Aziza, E. and Abdel, A. (2012). Effect of three insect growth regulators (chlorfluazuron, tebofenozoid and pyriproxyfen) on fecundity of S. littoralis, histopathological and some biochemical aspects of moth ovary. Egyptian Academic Journal of Biological Sciences. 4: 49-59.
    2. Chandi, A.K. and Singh, G. (2013). Susceptibility status of diamondback moth, Plutella xylostella (Linnaeus) to commonly used insecticides. Journal of Insect Science. 26(2): 188-191.
    3. Chandi, R.S., Chandi A.K., Singh G. and Suri K.S. (2012). Insecticide use pattern on cole crops in Punjab. Journal of Insect Science. 25: 210-13.
    4. Chapman, J. W., Reynolds, D. R., Smith, A. D., Riley, J. R., Pedgley, D.E. and Woiwod, I. P. (2002). High altitude migration of the diamondback moth Plutella xylostella to the UK: A study using radar, aerial netting and ground trapping. Ecological Entomology. 27: 641-50.
    5. Chu, Y. (1986). The migration of the diamondback moth. In: Diamondback Moth Management: Proc 1st Int Wkshop. (Talekar, N.S and Greggs, T. D. eds.). Asian Vegetable Research and Development Centre, Shanhua, Taiwan. pp. 77-81.
    6. Coulson, S.J., Hodkinson, I.D., Webb, N.R., Mikkola, K., Harrison, J.A. and Pedgley, D.E. (2002). Aerial colonization of high Arctic islands by invertebrates: the diamondback moth, Plutella xylostella (Lepidoptera: Yponomeutidae) as a potential indicator species. Diversity and Distriution. 8: 327-34.
    7. Fletcher, T.B. (1914). Some south Indian insects. Superintendent Government Press, Madras. 
    8. Gautam, M.P., Singh, H., Kumar, S., Kumar, V., Singh, G. and Singh, S.N. (2018). Diamondback moth, Plutella xylostella (Linnaeus) (Insecta: Lepidoptera: Plutellidae) a major insect of cabbage in India: A review. Journal of Entomology and Zoology Studies. 6(4): 1394-1399.
    9. Ghazawi, A.N., El-Shranoubi, E.D., El-Shazly, M.M. and Abdel Rahman, A. (2007). Effects of azadirachtin on mortality and reproductive system of the grasshopper, Heteracris littoralis Ramb. (Orthoptera : Acrididae). Journal of Orthoptera Research. 16(1): 57-65.
    10. Honda, K. (1990). Hibernation and migration of diamondback moth in northern Japan. In: Proc 2nd Int Wkshop (Talekar, N.S. and Greggs, T.D. eds.). Taiwan. pp. 43-50.
    11. Honda, K., Miyahara, Y. and Kegasawa, K. (1992). Seasonal abundance and the possibility of spring immigration of the diamondback moth Plutella xylostella (Linnaeus) (Lepidoptera: Yponomeutidae), in Morioka city, northern Japan. Appied Entomology and Zoology. 27: 517-25.
    12. Jallow, M.F.A and Hoy, C.W. (2007). Indirect selection for increased susceptibility to permethrin in diamondback moth (Lepidoptera: Plutellidae). Journal of Economic Entomology. 100(2): 526-33.
    13. Kaur, K. (2015). Sublethal effects of pyriproxyfen on tobacco caterpillar, Spodoptera litura (Fabricius). M.Sc Thesis. Punjab Agricultural University, Ludhina, India.
    14. Khaliq, A., Attique, M.N.R. and Sayyed, A. H. (2007). Evidence for resistance to pyrethroids and organophosphates in Plutella xylostella (Lepidoptera: Plutellidae) from Pakistan. Bulltein of Entomological Research. 97: 191-200.
    15. Lal, A. (1992). Studies on biology of diamondback moth, Plutella xylostella (Linn.) on cauliflower in Punjab. M.Sc. Thesis, Punjab Agricultural University, Ludhiana, India.
    16. Liang, G.M., Chen, W. and Liu, T.X. (2003). Effects of three neem-based insecticides on diamondback moth (Lepidoptera: Plutellidae). Crop Protection. 22: 333-40.
    17. Liu, S.S., Chen, F.Z. and Zalucki, M.P. (2002). Development and survival of the diamondback moth (Lepidoptera: Plutellidae) at constant and alternating temperatures. Environmenta Entomology. 31: 221-31.
    18. Loeb, M., Brandt, E.P. and Birnbaum, M.J. (1984). Ecdysteroid production by testes of budworm, Heliothis virescens from last larval instar to adult. Journal of Insect Physiology. 30: 375-81.
    19. Maiza, A., Kilani, S., Farine, J.P., Smagghe, G., Aribi, N. and Soltani, N. (2004). Reproductive effects in German cockroaches by ecdysteroid agonist RH-0345, juvenile hormone analogue methoprene and carbamate benfuracarb. Communication in Agriculture and Applied Biological Sciences. 69: 257–266.
    20. Meena, S. and Singh, N.P. (2014). Ultrastructural changes in female reproductive organ of Chrotogonus trachypterus Blanchard induced by deltamethrin. IOSR Journal of Agriculture and Veterinary Science. 7: 1-6.
    21. Mota-Sanchez, D., Bills, P.S. and Whalon, M.E. (2002). Arthropod resistance to pesticides: Status and overview. In: Pesticides in agriculture and the environment. (Wheeler, W.B. ed.). pp 241-272. Marcel Dekker Incorporation, New York.
    22. Moura, A.P., Carvalho, G.A. , Cosme, L.V., Alves, E., Botton, M. and Silva, P.S. (2011). Toxicological and ultrastructural analysis of the impact of pesticides used in temperate fruit crops on two populations of Chrysoperla externa (Neuroptera, Chrysopidae). Revista Brasileira de Entomologia. 55(3): 411–418.
    23. Nomura, M. and Miyata, T. (2000). Effects of pyriproxyfen, insect growth regulator on reproduction of Spodoptera litura, common cutworm. Japanese Journal of Applied Entomology and Zoology. 44: 81-88.
    24. Oliveira, A.C., Siqueira, H.A.A., Oliveira, J.V., Silva, J.E. and Filho, M.M. (2011). Resistance of Brazilian diamondback moth populations to insecticides. Scientia Agricola. 68(2):154-59. 
    25. Perlak, F.J., Stone, T.B., Muskipf, Y., Petersen, L.J., Parker, G.B., McPherson, S.A., Wyman, J., Love, S., Reed, G., Biever, D. and Fishhoff, D.A. (1993). Genetically improved potatoes: protection from damage by Colorado potato beetles. Plant Molecular Biology. 22: 313-21. 
    26. Perveen, F. and Miyata, T. (2000). Effects of sublethal dose of chlorfluazuron on ovarian development and oogenesis in the common cutworm Spodoptera litura (Lepidoptera: Noctuidae). Annals of Entomological Society of America. 93(5): 1131-37. 
    27. Santos, D.C.D. and Gregorio, E.A. (2002). Ultrastructure of the ovariole sheath in Diatraea saccharalis (Lepidoptera: Pyralidae). Biocell. 26: 229-35.
    28. Saxena, J.D., Rai, S., Srivastava, K.M. and Sinha, S.R. (1989). Resistance in the field populations of diamondback moth to some commonly used synthetic pyrethroids. Indian Journal of Entomology. 51: 265-268.
    29. Shelton, A.M. (2004). Management of diamondback moth: In: Proc 4th Int Wkshop, 26-29 November 2001. (Enderby, N.M. and Ridland, P.M. eds.). pp 38. Department of Natural Resources and Environment, Melbourne.
    30. Singh, K.R., Kaur, R., Chandi, A.K. and Singh, G. (2017). Influence of spinosad on reproduction of diamond backmoth Plutella xylostella (Linnaeus). Indian Journal of Entomology. 79(4): 467-473.
    31. Verkerk, R.H.J. and Wright, D.J. (1996). Multi-trophic interactions and management of the P. xylostella. A review. Bulletin of Entomological Research. 86: 205-216.
    32. Xu, Q., Tang, B., Zou, Q., Zheng, H., Liu, X. and Wang, S. (2015). Effects of pyriproxyfen on female reproduction in the common cutworm, Spodoptera litura (F.) (Lepidoptera: Noctuidae). PLoS ONE. 10(10): e0138171.
       
    In this Article
      Contact us
      Follow us
      Published In
      Agricultural Science Digest

      Editorial Board

      View all (0)