Loading...

Phenotypic and Molecular Characterization of the Capsular Serotypes of Pasteurella multocida Isolated from Pneumonic Cases of Cattle in Ethiopia

DOI: 10.18805/ag.D-333    | Article Id: D-333 | Page : 365-370
Citation :- Phenotypic and Molecular Characterization of the Capsular Serotypes of Pasteurella multocida Isolated from Pneumonic Cases of Cattle in Ethiopia.Agricultural Science Digest.2022.(42):365-370
Y.A. Mirtneh, B.M. Vemulapati, A. Takele, Y. Martha, D. Teferi, B. Alebachew, D. Getaw, G. Esayas mirtneh2010@gmail.com
Address : Department of Biotechnology, Koneru Lakshmaiah Education Foundation, Vaddeswaram, Gunture-522 502, Andhra Pradesh, India. 
Submitted Date : 29-01-2021
Accepted Date : 22-04-2021

Abstract

Background: Pasteurella multocida is a heterogeneous species and opportunistic pathogen associated with pneumonia in cattle. Losses due to pneumonia and associated expenses are estimated to be higher in Ethiopia with limited information about the distribution of capsular serotypes. Hence, this study was designed to determine the biotypes and capsular serotypes of P. multocida from pneumonic cases of cattle.
Methods: A cross-sectional study with a purposive sampling method was employed in 400 cattle from April 2018 to January 2019. Nasopharyngeal swabs and lung tissue samples were collected from clinically suspected pneumonic cases of calves (n = 170) and adult cattle (n = 230). Samples were analyzed using the bacteriological and molecular assay.
Result: Bacteriological analysis revealed isolation of 61 (15.25%) P. multocida subspecies multocida. Molecular assay targeting KMT1 gene (~460 bp) confirmed P. multocida species. Capsular typing revealed the presence of serogroup A (hyaD-hyaC) gene (~1044 bp) and serogroup D (dcbF) gene (~657 bp) in 56 (91.80%) and 5 (8.20%) isolates, respectively. Isolation of P. multocida A:3 highlights its prevalence in the study areas. Therefore, the current finding suggests further comprehensive studies on P. multocida capsular types and microbial diversity of respiratory infection in cattle to design an effective control strategy.

Keywords

Biovar Capsular type Cattle Ethiopia Pasteurella multocida

References

  1. Abera, D., Sisay, T. and Birhanu, T. (2014). Isolation and identification of Mannhemia and Pasturella species from pneumonic and apparently healthy cattle and their antibiogram susceptibility pattern in Bedelle District, Western Ethiopia. African Journal of Bacteriology Research. 6(5): 32-41.
  2. Carter, G.R. (1955). Studies on Pasteurella multocida. I.A hemagglutination test for the identification of serological types. American Journal of Veterinary Research. 16(60): 481-484. 
  3. Dabo, S.M., Taylor, J.D. and Confer, A.W. (2007). Pasteurella multocida and bovine respiratory disease. Animal Health Research Reviews. 8(2): 129-150. 
  4. Ewers, C., Lübke-Becker, A., Bethe, A., Kießling, S., Filter, M. and Wieler, L.H. (2006). Virulence genotype of Pasteurella multocida strains isolated from different hosts with various disease status. Veterinary microbiology. 114: 304-317. 
  5. Gebremeskel, A.K., Tesema, T.S., Yegoraw, A.A., Birhanu, B.T. and Mekuria, S.A. (2017). Isolation and Characterization of Bacterial Species from Respiratory Tracts of Cattle Slaughtered in Addis Ababa City, Central Ethiopia. World Veterinary Journal. 7(1): 14-20.
  6. Harper, M., Boyce, J.D. and Adler, B. (2006). Pasteurella multocida pathogenesis: 125 years after Pasteur. FEMS microbiology letters. 265(1): 1-10. 
  7. Harper, M., John, M., Turni, C., Edmunds, M., Michael, F.S., Adler, B. and Boyce, J.D. (2015). Development of a rapid multiplex PCR assay to genotype Pasteurella multocida strains by use of the lipopolysaccharide outer core biosynthesis locus. Journal of Clinical Microbiology. 53(2): 477-485.
  8. Heddleston, K.L., Gallagher, J.E. and Rebers, P.A. (1972). Fowl cholera: gel diffusion precipitin test for serotyping Pasteurella multocida from avian species. Avian diseases. 16(4): 925-936. 
  9. Jonathan, L. and Arora, A.K. (2012). Comparison of Detection Threshold of Different Pasteurella Multocida Specific PCRS. Indian Journal of Animal Research. 46(1): 28-33.
  10. Katsuda, K., Hoshinoo, K., Ueno, Y., Kohmoto, M. and Mikami, O. (2013). Virulence genes and antimicrobial susceptibility in Pasteurella multocida isolates from calves. Veterinary microbiology. 167(3-4): 737-741.
  11. Kim, J., Kim, J.W., Oh, S.I., So, B., Kim, W.I. and Kim, H.Y. (2019). Characterisation of Pasteurella multocida isolates from pigs with pneumonia in Korea. BMC veterinary research. 15(1): 1-9.
  12. Kong, L.C., Wang, Z., Wang, Y.M., Dong, W.L., Jia, B.Y., Gao, D. and Ma, H. X. (2019). Antimicrobial susceptibility and molecular typing of Pasteurella multocida isolated from six provinces in China. Tropical animal health and production. 51(4): 987-992. 
  13. Musteria M., Getnet F., Anteneh W. and Yehualashet B. (2017). Isolation of Pasteurella multocida and Mannheimia hemolytica from Pneumonic Calves and Their Antibiotic Susceptibility in Harar and Haramaya, Eastern Ethiopia. Global Veterinaria. 19(5): 627-632. 
  14. Peek, S.F., Ollivett, T.L. and Divers, T.J. (2018). Respiratory diseases. Rebhun’s Diseases of Dairy Cattle. pp. 112.
  15. Quinn, P.J., Carter, M.E., Markey, B. and Carter, G.R. (2002). Clinical Veterinary Microbiology and microbial disease, Blackwell sciences. Publishing Wolf Spain. pp. 261-267.
  16. Taylor, J.D., Fulton, R.W., Dabo, S.M., Lehenbauer, T.W. and Confer, A.W. (2010). Comparison of genotypic and phenotypic characterization methods for Pasteurella multocida isolates from fatal cases of bovine respiratory disease. Journal of veterinary diagnostic investigation. 22(3): 366-375. 
  17. Townsend, K.M., Boyce, J.D., Chung, J.Y., Frost, A.J. and Adler, B. (2001). Genetic organization of Pasteurella multocida cap loci and development of a multiplex capsular PCR typing system. Journal of clinical microbiology. 39(3): 924-929.
  18. Townsend, K.M., Frost, A.J., Lee, C.W., Papadimitriou, J.M. and Dawkins, H.J. (1998). Development of PCR assays for species-and type-specific identification of Pasteurella multocida isolates. Journal of clinical microbiology. 36(4): 1096-1100.
  19. Wilson, B.A. and Ho, M. (2013). Pasteurella multocida: from zoonosis to cellular microbiology. Clinical microbiology reviews. 26(3): 631-655. 

Global Footprints