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Agricultural Science Digest, volume 31 issue 1 (march 2011) : 38 - 39

AGROBACTERIUM RHIZOGENE MEDIATED TRANSFORMATION OF NIGER [GUIZOTIA ABYSSINICA (L.F.) CASS.]

B.T. Dangat, A.R. Patil

Affiliations

¹Department of Botany, Rajaram College, Kolhapur - 416 004, India.

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Cite article:- Dangat B.T., Patil A.R. (2024). AGROBACTERIUM RHIZOGENE MEDIATED TRANSFORMATION OF NIGER [GUIZOTIA ABYSSINICA (L.F.) CASS.]. Agricultural Science Digest. 31(1): 38 - 39. doi: .

ABSTRACT

A protocol was developed for Agrobacterium rhizogene mediated transformation of Niger (Guizotia abyssinica (L.f.) Cass.). Co-cultivation of cotyledons from one week old plantlets with Agrobacterium rhizogene strain ATCC15834 induces negatively geotropic hairy roots directly from explants without intermediate stage i.e. callus on MS medium, supplemented with 1 mg/l of 6-Benzyl amino purine (BAP). Transformation was confirmed on the basis of formation of negatively geotropic hairy roots compared with control.

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REFERENCES

Ashok Kumar H.G., H.N. Murty and V.G.Jadimath (2000). Direct somatic Embryogenesis and plantlet regeneration from leaf explant of Niger Guizotia abyssinica (L.f.)Cass.Indian J. Exp. Biol. 38 :1073-1075.
Anne Laure, Moyne, Dennis Tagu, Veronique (1989). Transformation calli obtained by direct gene transfer into sunflower protoplast. Plant Cell Reports 8: 97-100.
Miao Cheng Ying, William E. Dyer and Jerald W. Bergman(1992) Agrobacterium tumefaciens mediated transformation of Safflower( Carthamus tinctorius L.)CV. Centnnial Plant Cell Reports 11: 581-585.
Murthy, H.N., J.H. Jeong, Y.E. Choi and K.Y.Paek (2003). Agrobacterium mediated transformation of Niger (Guizotia abyssinica (L.f.)Cass.using seedling as explants.Plant Cell Reports 21:1183-1187.
Sarvesh A., T.P. Reddy and P.B.Kavikishore (1993).Plant regeneration from cotyledons of Niger Plant .Cell Tissue Organ Culture 32:131-135.

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AGROBACTERIUM RHIZOGENE MEDIATED TRANSFORMATION OF NIGER [GUIZOTIA ABYSSINICA (L.F.) CASS.]

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