Agricultural Science Digest

  • Chief EditorArvind kumar

  • Print ISSN 0253-150X

  • Online ISSN 0976-0547

  • NAAS Rating 5.52

  • SJR 0.156

Frequency :
Bi-monthly (February, April, June, August, October and December)
Indexing Services :
BIOSIS Preview, Biological Abstracts, Elsevier (Scopus and Embase), AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Agricultural Science Digest, volume 25 issue 4 (december 2005) : 244 - 247

IN VITRO MICROPROPAGATION STUDIES IN SUGARCANE

T.J. BhoT, H.B. Mungse
1College of Agriculture, Pune - 411 005. India

  • Submitted|

  • First Online |

  • doi

Cite article:- BhoT T.J., Mungse H.B. (2024). IN VITRO MICROPROPAGATION STUDIES IN SUGARCANE. Agricultural Science Digest. 25(4): 244 - 247. doi: .

ABSTRACT

The MS (Murashige and Skoog, 1962) medium supplemented with different levels of BAP (6, Benzyl ammo purine) (0.1, 0.2, 1.0, 2.0, 3.0 mg/l) and IBA (Indol-3-butyric acid) (0.1, 0.5, 1.0, 2.0 mg/l) with NAA (Napthaline acetic acid) (0.2, 0.3, 0.5 mg/l) were prepared separately and used for inoculation and subculturing as and when required. The establishment of shoot apex culture was observed on me media containing MS + 0.2 mg/l BAP + 0.2 mg/l NAA in the variety CoM-88121 (70%) and Co-86032 (60%). The maximum number of shoots (9.8 shoots in Co-86032 and 8.1 shoots in CoM-88121) were observed in me medium MS + 1.0 mg/l BAP + 0.5 mg/l NAA. The maximum number of roots were observed in the culture medium ½ MS + 1 mg/l IBA + 0.3 mg/l NAA. For hardening of the plantlets the relative humidity of 70–90% and 28–32°C temperature was maintained for initial 8 to 12 days and within such period the new roots were developed to these plantlets. The 60 days old spellings were planted in the field.

KEYWORDS

    REFERENCES

    1. Gonzalez. M.A. etal. (1985). Centro Agricola.. 12:136-136.
    2. Grisham. M.P. and Bourg. D. (1989). J. Am. Soc:;. Sugarcane Technol.. 9: 97-102.
    3. Hendre, R.R. et al. (1983). Sugarcane No.1: 5-8.
    4. Liu. M.e. (1971). Taiwan Sugar, 18: 8-10.
    5. Mohotkar. L.e. et al. (1993). Curro Sci.. 64: 604-605.
    6. Murashige. T. and Skoog. F. (1962). Physiolgia Plantarum.. 15: 473-497.
    7. Naik. G.R. and Babu. K.H. (1988). Curl'. Sci.. 57: 432-434.
    8. Rodriguez. A.J. (1982). Ciencias de la Agric. No. 11: 117-118.
    Reviewed By

    Download Article
    In this Article
      Contact us
      Follow us

      Editorial Board

      View all (0)