Loading...

Detection of Growth Hormone (GH) Gene Polymorphism in Norduz Sheep

DOI: 10.18805/IJAR.BF-1518    | Article Id: BF-1518 | Page : 1071-1076
Citation :- Detection of Growth Hormone (GH) Gene Polymorphism in Norduz Sheep.Indian Journal of Animal Research.2022.(56):1071-1076
Nzar Ibrahim Yousıf, Hasan Koyun hkoyun@yyu.edu.tr
Address : Department of Animal Science, Faculty of Agricultural, Biometry and Genetics Unit, Van-Yuzuncu Yil University, Turkey.
Submitted Date : 22-03-2022
Accepted Date : 25-06-2022

Abstract

Background: The Norduz sheep is a local breed of sheep bred for its meat, milk, progeny and fleece and is one of the genetic resources in the rural areas of Norduz, a district of the Turkish province of Van. The PCR-based method involves amplification of a conserved DNA region and digestion of the PCR products with DNA restriction endonucleases throughout the genome. Based on the resulting polymorphisms, statistical methods can be used to identify the desired genotypes among the alleles found. The objective of this study was to identify the polymorphisms of the growth hormone gene and determine their association with six growth traits in Norduz sheep. 
Methods: Out of 50 Norduz sheep, three different loci, GHY1, GHY2 and GHY3 (with the length of 599, 690 and 679 bp respectively) of GH gene on chromosome 11 of sheep genome were selected to identify polymorphisms among individuals using the PCR-RFLP technique (GH/MspI). 
Result: In this research study, no polymorphisms were detected after digesting three loci of the GH gene with the enzyme MspI. The 50 individuals of Norduz sheep had only AB genotypes for all three loci, indicating that no polymorphisms were present in the regions of the GH genes studied.

Keywords

Genetic resources Growth hormone (GH) gene Norduz sheep PCR-RFLP

References

  1. Afifi, M., Alkaladi, A., Kadasa, N. M. and Saddick, S. (2019). Sequencing and Polymorphism detection in growth hormone gene in Najdi Sheep and their association with milk production. Indian Journal of Animal Research. 53: 423-428. DOI: 10.18805/ijar.B-920.
  2. Al-Barzinji, Y.M.S. and Othman, G.U. (2013). Genetic polymorphism in FecB gene in iraqi sheep breeds using RFLP-PCR technique. Journal of Agriculture and Veterinary Science. Volume 2, Issue, 46-48. DOI: 10.9790/2380-0244648.
  3. Aygün, T., Bingöl, M., Akkaraman varyetesi Karakaş ve Norduzların doğum ağırlıkları bakımından karşılaştırılması. Ege Ün. Ziraat Fak Uluslararası Hayvancılık Kongresi 1999; sayfa: 738-742 (In Turkish). 
  4. Aygün, T., Bingöl, M., Gökda,l Ö. (2006). Comparison of feedlot performance and carcass traits of tail docked Karakas and Norduz male lambs. Indian Veterinary Journal. 83: 869-872.
  5. Bozhilova-Sakova, M. and Dimitrova, I. (2016). “PCR-RFLP Analysis of CAST Gene in one Bulgarian Sheep Breed”, Athens: Atýner’s Conference Paper Series. No: AGR2015-1824.
  6. Depison, Anwar, S., Jamsari and Yurnalis. (2017). Association of growth hormone gene polymorphism with quantitative characteristics of thin-tailed sheep using PCR-RFLP in Jambi province. African Journal of Biotechnology. 1159- 1167. doi: 10.5897/AJB2016.15783.
  7. Abdelmoneim, T.S., Brooks, P.H.,  Afifi, M. and Abdel-Aziz Swelum, A. (2016). Sequencing of growth hormone gene for detection of polymorphisms and their relationship with body weight in Harri sheep. Indian Journal of Animal Research. 51 (2) 2017: 205-211. DOI:10.18805/ijar. 11457.
  8. Dimitrova and Bozhilova-Sakova, M. (2016). PCR-RFLP Analysis of Callipyge gene (Clpg) in Karakachan sheep breed. Bulgarian Journal of Agricultural Science. 22(3): 482-484.
  9. Jamshidi, R., Kasirian, M.M. and Rahimi, G.A. (2013). Application of PCR-RFLP technique to determine Booroola gene polymorphism in the Sangsari sheep breed of Iran. Turkish Journal of Veterinary and Animal Sciences. 37: 129-133. doi.org/10. 3906/vet-0806-14.
  10. Koyun, H., Kiraz, S., Karaca, Koncagül, S., Yılmaz, A., Karakuş, K., Yeşilova, A., Aygün, T. (2019 ). Determination of Milk Protein Polymorphism in Norduz and Karakaş Sheep Breeds using PCR-RFLP Technique. 1st International Göbeklitepe Agriculture Congress. IGAC-2019 , 417-421.
  11. Kumari, R., Kumar, R., Kumar, S., Jyotsana, B., Meena, A. and Prince, L. (2014). Genetic polymorphism of growth hormone gene in native sheep breeds of India. The Indian Journal of Small Ruminants. 20(2): 15-18.
  12. Mahrous, K.F., Abdel-Aziem, S.H., Abdel-Hafez, M.A., Abdel-Mordy, M. and Rushdi, H.E. (2018). Polymorphism of growth hormone gene in three goat breeds in Egypt. Bulletin of the National Research Centre. 1-7. doi.org/10.1186/ s42269-018-0035-0.
  13. Mahmood., M.R. (2018). Growth hormone (GH) gene polymorphisms in Norduz sheep. ( M.Sc. Thesis)., Van-YYÜ. Fen. Bil. Enst, Van.
  14. Meena, A.S. , Bhatt, R.S., Sahoo, A. and Kumar, S. (2016). Polymorphism of the exon 3 of leptin gene in Malpura sheep. Indian Journal of Animal Research. 51(3): 2017: 469-473. DOI:10.18805/ijar.v0i0f.3783.
  15. Moradian, C., Mohamadi, N., Razavi-Sheshdeh, S.A., Hajihosseinlo, A. and Ashrafi, F. (2013). Effects of genetic polymorphism at the growth hormone gene on growth traits in Makooei sheep. European Journal of Experimental Biology. 3(3): 101-105. ISSN: 2248 -9215.
  16. Nanekarani, S., Goodarzi, M. and Mahdavi, M. (2014) Analysis of Polymorphism of Callipyge Gene in Lori Sheep by PCR- RFLP Method. 4th International Conference on Agriculture and Animal Science. (CAAS 2013), APCBEE Procedia 8 ( 2014 ) 65-69.
  17. Phookan, A., Sarma, M., Khanikar, D., Medhi, D. and Deori, S. (2021). Detection of polymorphism of FecG gene in indigenous sheep of Arunachal Pradesh. The Pharma Innovation Journal.  SP-10(2): 156-159. ISSN (E): 2277- 7695.
  18. Rasmussen, H.B. (2012). Restriction Fragment Length Polymorphism Analysis of PCR-Amplified Fragments. Gel Electrophoresis- principles and Basics. https://www.intechopen.com/ chapters/35104 316-334. DOI: 10.5772/37724.
  19. Seevagan, M., Jeichitra, V., Rajendran, R. and Tirumurugaan, K. (2015). Polymorphism of growth hormone (GH) gene in vembur and kilakarsal breeds of sheep. Indian Journal of Small Ruminants. 21(2): 196. DOI: 10.5958/0973- 9718. 2015.00044.6.
  20. Tolee, A.R.R., Olga, E. and Ekaterina, C. (2021). Identification of CLPG gene polymorphism using PCR-RFLP of Iraq and Belarus population sheep breeds. Gene Reports. 22: 100974. doi.org/10.1016/j.genrep. 2020.100974.
  21. Yılmaz, O., Wilson, R.T., Kor, A., Cengiz, F. and Ertuğrul, M. (2012). The farm animal genetic resources of Turkey: sheep - I- common and rare breeds. Bitlis Eren University Journal of Science and Technology. 2(1): 1-5. ISSN 2146-7706.

Global Footprints