Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

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Full Research Article

Transcriptomic Response to Sudden Salinity Drop in the Liver of Juvenile Scatophagus argus

X.N. Sun, C.C. Shen, G.P. Feng, J.Y. Liu, C. Song, Y.F. Yu

ABSTRACT

Background: Scatophagus argus is a euryhaline fish, but the molecular mechanism of salinity plunge response on liver antioxidant system of S. argus is currently poorly understood. Meanwhile, the transcriptome is a powerful tool for studying the effect of stress on physiological mechanisms. Transcriptome analysis of juvenile S. argus in salinity slump will provide a reference for future research on the stress resistance of S. argus.
Methods: 5 ppt was selected as low salinity and 20 ppt was used as the control group. The experimental treatment time was 6 h. Liver samples were separately obtained for transcriptome sequencing. Transcriptomic data of the liver after salinity plunge for 6 h were analyzed.
Result: 474 differentially expressed genes were generated by the sudden change of salinity. The pathways related to immune defense were enriched, such as ‘Antigen processing and presentation’ and ‘Phagosome’, implying that S. argus might enhance the immune defense system. Several antioxidant enzyme genes, such as HAO, Trx, PHGPx, were up-regulated to resist oxidative stress caused by low salinity. The down-regulation of Cul3 also might promote the activation of Nrf2 to activate the expression of antioxidant enzyme genes. These results indicated that S. argus initiated a molecular mechanism to resist low salt stress.

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