Indian Journal Of Animal Research

Effects of eprinomectin administration on apoptosis, acute phase response and antioxidant status in cattle

DOI: 10.18805/ijar.B-996    | Article Id: B-996 | Page : 413-418
Citation :- Effects of eprinomectin administration on apoptosis, acute phase response and antioxidant status in cattle.Indian Journal Of Animal Research.2020.(54):413-418
H. Aksit and D. Aksit hasanaksit@balikesir.edu.tr
Address : Department of Biochemistry, Faculty of Veterinary, Balikesir University, Balikesir, Turkey.
Submitted Date : 4-06-2018
Accepted Date : 29-10-2018
First Online:

Abstract

Eprinomectin is a broad spectrum endectocides used against gastrointestinal, pulmonary nematodes and ectoparasites in cattle. The main objective of the present study was to investigate effects of eprinomectin in cows following subcutaneous and pour-on administrations; on serum DNA apoptosis, acute phase response, Total Antioxidant Status (TAS), total protein, creatinine, urea levels, and AST, ALT and GGT activities. Ten Holstein cows were divided into two groups. Group 1 received subcutaneous (0.2 mg/kg) and group 2 received pour-on (0.5 mg/kg) administration of eprinomectin. Blood samples were collected from vena jugularis prior to and following the drug administration at intervals of 36 hrs, 3rd, 6th, 12nd and 20th days, respectively. Samples were centrifuged at 2500 g for 15 minutes and separated sera stored at -20 ºC. Results showed that the eprinomectin application decreases the sera levels of acute phase proteins. Statistically significant increase was observed  in antioxidant capasity (P<0.05) and total protein (P<0.01). However, there was non-significant increase in apoptosis rate. No remarkable alterations were observed in AST, ALT and GGT activities including urea and creatinine levels. Results showed that the eprinomectin application reduces  the sera levels of acute phase proteins (ceruloplasmin and sialic acid) in cows. However, statistically significant increase  was measured in antioxidant capasity (P<0.05) and total protein (P<0.01) levels. It could be concluded from the study that eprinomectin do not have adverse effects on liver and kidney. 

Keywords

Acute phase proteins Antioxidants Apoptosis Eprinomectin.

References

  1. Aksakal, M. and Özer, E. (1987). Akkaraman koyunlarýnda antelmentik ilaçlarla tedaviden önce ve sonra hematolojik deðerler ve kan plazmasý vitamin E düzeyi üzerine araþtýrmalar. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 34:72-84.
  2. Aksit, H. and Bildik, A. (2008). Apoptozis. Yüzüncü Yýl Üniversitesi Veteriner Fakültesi Dergisi, 19:55-63.
  3. Aouffen, M., Paquin, J., Furtos, A., Waldron, K.C. and Mateescu, M.A. (2004). Oxidative aggregation of ceruloplasmin induced by hydrogen peroxide is prevented by pyruvate. Free Radical Research, 38:19-26.
  4. Arslan, H.H., Nisbet, C., Sarýpýnar, D., Çenesiz, S. and Çenesiz, M. (2008). Effects of combination of Acetyl methionine, L-Carnitine, vitamin E and vitamin B on some clinical, haematological and biochemical parameters in cattle. YYÜ Veteriner Fakültesi Dergisi, 19:9-14.
  5. Arthington, J.D., Eicher, S.D. and Kunkle, W.E. (2003). Effect of transportation and commingling on the acute-phase protein response, growth, and feed intake of newly weaned beef calves. Journal of Animal Science, 81:1120-1125.
  6. Atakisi, E., Atakisi, O., Topcu, B. and Uzun, M. (2009). Effects of therapeutic dose of ivermectin on plasma nitric oxide and total antioxidant capacity in rabbits. European Review for Medical and Pharmacological Science, 13:425-429.
  7. Azab, M.E. and Abdel-Maksoud, H.A. (1999). Changes in some haematological and biochemical parameters during prepartum and postpartum periods in female baladi goats. Small Ruminant Research, 34:77-85.
  8. Baspinar, N. and Serpek, B. (1993). Gebe koyunlarda vitamin C, seruloplazmin, glikoz ve hemoglobin deðerlerinin postpartum ilk aya kadar deðiþimleri ve bu parametreler arasýndaki iliþkiler. Hayvancýlýk Araþtýrma Enstitüsü Dergisi, 3:88-92.
  9. Bilgili, A., Kart, A., Cigremis, Y., Hanedan, B. and Erdag, D. (2009). A comparative study on the effects of eprinomectin and ivermectin on plasma antioxidant level and lipid peroxidation in cows. Kafkas Universitesi Veteriner Fakültesi Dergisi, 15(2):287-292.
  10. Ci, X.X., Li, H.Y., Yu, Q.L., Zhang, X.M., Yu, L., Chen, N., Song, Y. and Deng, X. (2009). Avermectin exerts anti-inflammatory effect by downregulating the nuclear transcription factor kappa-B and mitogen-activated protein kinase activation pathway. Fundamental and Clinical Pharmacology, 23:449-455.
  11. Çorum, D.D. and Kart, A. (2017). The protective role of silymarin against methotrexate induced gastrointestinal injury. Indian J. Anim. Res., 51(2):401-405. 
  12. Cotran, R.S., Kumar, V., Collins, T. and Robbins, S.L. (1999). Robbins and Pathologic Basis of Disease. Ed.W.B. Saunders Company 6th ed; Philadelphia, PA, USA. 
  13. Dede, S., Deðer, Y., Deðer, S. and Aklan, M. (2000). Determination of the status of lipid peroxidation and antioksidant in sheep infected with certain endoparasites (Fasciola sp, Trichostrongylidae sp., Eimeria sp.). Türkiye Parazitoloji Dergisi, 24:190-193.
  14. Deðer, Y., Gül, A., Bildik, A., Dede, S., Yur, F. and Deðer, S. (1997). Parazitli köpeklerin bazý kan parametreleri ile plazma vitamin C düzeylerinde görülen deðiþiklikler. Türkiye Parazitoloji Dergisi, 21:195-198.
  15. Eckarsall, P.D. (2000). Recent advances and future prospects for the use of acute phase proteins as marker of disease in animals. Revue de Medecine Veterinaire, 151:577-584.
  16. El Gohari, Y., Galal, S.H., Boulos, L.M., Moustafa, S., Amin, S.M. and El Talebany, A.M. (1984). Trace elements in some parasitic diarrhea. Journal of the Egyptian Society Parasitology, 14:179-187.
  17. Gameel, A.A. (1982). Fasciola hepatica: plasma ascorbic acid, plasma iron and iron binding capacity in experimentally infected sheep. Zeitschrift für Parasitenkunde, 68:185-189.
  18. Gökmen, S.S., Sunar, B., Kazezoðlu, C., Özçelik, F., Yorulmaz, F. and Gülen, Þ. (2004). Akut myokard infarktüsünde inflamasyona duyarlý protein düzeyleri. Turkish Journal Biochemistry, 29:256-261.
  19. Güngör, Ö., Sunar, B., Özçelik, F., Aktaþ, Z. and Gökmen, S.S. (2004). Serum sialic acid levels in acute myocardial infarction and its relationship to ceruloplasmin. Turkish Journal Biochemistry, 29:226-231.
  20. Hammond, C.L., Marchan, R., Krance, S.M. and Ballatori, N. (2007). Glutathione export during apoptosis requires functional multidrug resistance-associated proteins. The Journal of Biological Chemistry, 282:14337-14347.
  21. Haris, E.D. (1991). Copper transport: an overview. Experimental Biology and Medicine, 196:130-140.
  22. Jin, Y., Zhang, X., Shu, L., Chen, L., Sun, L., Qian, H., Liu, W. and Fu, Z. (2010). Oxidative stress response and gene expression with atrazine exposure in adult female zebraûsh (Danio rerio). Chemosphere, 78:846–852.
  23. Joreno, D.R. (1990). Malondialdehyde and thiobarbituric acid reactivity as perostic indices of lipid peroxidation and peroxidative tissue injury. Free Radical Biology and Medicine, 9:515-540.
  24. Kabu, M., Cigerci, I.H., Uyarlar, C. and Celik, H.A. (2015). Determination of pre and post treatment oxidative status and oxidative DNA damage in diarrheic calves. Indian J. Anim. Res., 49(6):830-833.
  25. Kanbur, M., Atalay, O., Ica, A., Eraslan, G. and Cam, Y. (2008). The curative and antioxidative efficiency of doramectin and doramectin + vitamin AD3E treatment on Psoroptes cuniculi infestation in rabbits. Research in Veterinary Science, 85:291-293.
  26. Karwatsky, J., Lincoln, M.C. and Georges, E. (2003). A mechanism for P-glycoprotein-mediated apoptosis as revealed by verapamil hypersensitivity. Biochemistry. 42:12163-12173.
  27. Kaymaz, A.A., Bakýrel, U., Gönül, R., Tan, H. and Vuruþaner, C. (1999). Serum protein electrophoresis in dogs with intestinal parasites. Turkish Journal of Veterinary And Animal Sciences. 23:457-459.
  28. Keleþ, I., Ertekin, A., Karaca, M., Ekin, S. and Akkan, H.A. (2000). Sýðýrlarýn leptospirozisinde serum sialik asit ve lipid-baðlý sialik asit düzeyleri üzerine araþtýrma. Yüzüncü Yýl Üniversitesi Veteriner Fakültesi Dergisi. 11:121-122.
  29. Kozan, E., Avcý, G., Sevimli, F.K., Birdane, F.M. and Köse, M. (2010). Determine of the antioxidant levels and some biochemical parameters on infected with ascaridiosis and treated dogs. Ankara Üniversitesi Veteriner Fakültesi Dergisi. 57:93-97.
  30. Mattson, M.P. and Chan, S.L. (2003). Calcium orchestrates apoptosis. Nature Cell Biology. 5:1041-1043.
  31. Meglia, G.E., Johannisson, A., Petersson, L. and Waller, P. (2001). Changes in some blood micronutrients, leukocytes and neutrophil expression of adhesion molecules in periparturient dairy cows. Acta Veterinary Scandinavica. 42:139-150.
  32. Nakadai, A., Li, Q. and Kawada, T. (2006). Chlorpyrifos induces apoptosis in human monocyte cell line U937. Toxicology. 224:202–209.
  33. Noori, M., Azar, A.M., Saidi M., Panahandeh, J. and Haghi, D.Z. (2018). Evaluation of water deficiency impacts on antioxidant enzymes activity and lipid peroxidation in some tomato (Solanum lycopersicum L.) Lines. Indian J. Agric. Res. 52(3):228-235.
  34. Prabhavathy, H. and Palanivel, K.M. (2015). Ability of Indian street rabies virus isolates to induce apoptosis by in-vitro. Indian J. Anim. Res. 49(5):612-617.
  35. Sardesai, V.M. (1995). Role of antioxidants in health maintenance. Nutrition in Clinical Practice. 10:19-25.
  36. Sarin, K., Kumar, A., Prakash, A. and Sharma, A. (1993). Oxidative stress and antioxidant defence mechanism in Plasmodium vivax malaria before and after chloquin treatment. Indian Journal of Malariolog. 30:127-133.
  37. Shoop, W.L., Egerton, J.R., Eary, C.H., Haines, H.W., Michael, B.F., Mrozik, H., Eskola, P., et al (1996). Eprinomectin: a novel avermectin for use as a topical endectocide for cattle. International Journal for Parasitology. 26:1237-1242. 
  38. Soulsby, E.J.L. (1982). Helmints, Arthropods and Protozoa of Domesticated Animals. 7th ed; Bailliere Tindal London,. 149-155. 
  39. Sunderman, F.W. and Nomoto, S. (1970). Measurement of human serum ceruloplasmin by its p-phenylenediamine oxidase activity. Clinical Chemistry. 16:903-910.
  40. Sydow, G., Wittmann, W., Bender, E. and Starick, E. (1988). The sialic acid content of the serum of cattle infected with bovine leukosis virus. Archiv für experimentelle Veterinärmedizin. 42:194-197.
  41. Taylor, M.A. (2001). Recent developments in ectoparasiticides. The Veterinary Journal. 161:253-268.
  42. Wang, G. and Wu, R. (2017). Comparative analysis of the differential expressed proteins in serum between healthy and Brucella ovis infected sheep. Indian J. Anim. Res. 51(5):910-915.
  43. Wang, J.J., Cui, Y.L., Li, J.M., Jia, H.Y., Yu, Q. and Li, S. (2009). Effects of avermectin on level of intracellular free calcium and expression of CaM mRNA in King Pigeon neurons cultured in vitro. Chinese Journal of Veterinary Science. 39:75-78.
  44. Wang, M., and Li, S. (2008). Effect of avermectin on mitochondria from primary cultured neurons. Acta Sci Circum. 28:2101-2104.
  45. Warren, L. (1959). The thiobarbituric acid assay of sialic acids. Journal of Biological Chemistry. 234:1971-1975. 
  46. Weissman, S.M., Wochner, R.D., Mullins, F.X., Wynngate, A. and Waldmann, T.A. (1966). Synthesis of plasma proteins by hepatectomized dogs. American Journal of Physiology. 210:128-132.
  47. Yamamoto, S., Shida, T., Miyaji, S., Santsuka, H., Fujise, H., Mukawa, K., Furukawa, E., Nagae, T. and Naiki, M. (1993). Changes in serum C-reactive protein levels in dogs with various disorders and surgical traumas. Veterinary Research Communications.17:85-93.
  48. Yarsan, E., Altýnsaat, Ç., Ayçiçek, H., ªahindokuyucu, F. and Kalkan, F. (2003). Effects of albendazole treatment on haematological and biochemical parameters in healthy and Toxocara canis infected mice. Turkish Journal of Veterinary and Animal Sciences. 27:1057-1063.
  49. Zhu, W.J., Li, M., Liu, C., Qu, J.P., Min, Y.H., Xu, S.W. and Li, S. (2013). Avermectin induced liver injury in pigeon: Mechanisms of apoptosis and oxidative stress. Ecotoxicology and Environmental Safety. 98:74-81.

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