Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 54 issue 5 (may 2020) : 631-634

Glanders in horses in some selected areas of Bangladesh and comparison between CFT and Immunoblot used for the screening of glanders

Md. Siddiqur Rahman, Palash Kumar Bhattacharjee, Roma Rani Sarker, Mst. Sonia Parvin, Sayra Tasnin, M.A.S. Sarker, Heinrich Neubauer, Fahima Khatun, Md. Abdul Wares, Izumi Nishidate, Mandy C. Elschner
1Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh.
Cite article:- Rahman Siddiqur Md., Bhattacharjee Kumar Palash, Sarker Rani Roma, Parvin Sonia Mst., Tasnin Sayra, Sarker M.A.S., Neubauer Heinrich, Khatun Fahima, Wares Abdul Md., Nishidate Izumi, Elschner C. Mandy (2018). Glanders in horses in some selected areas of Bangladesh and comparison between CFT and Immunoblot used for the screening of glanders. Indian Journal of Animal Research. 54(5): 631-634. doi: 10.18805/ijar.B-976.

ABSTRACT

Glanders is a fatal infectious and notifiable zoonotic disease of equines caused by the Gram-negative non-motile bacterium Burkholderia (B.) mallei, which is responsible for chronic suppurative lesions of the skin and mucous membranes, pneumonia and septicemia in equines. Glanders in horses is worldwide distributed and reported from many countries. But no prevalence study was done in Bangladesh so far. Therefore, this preliminary study was conducted to determine the seroprevalence of glanders in horses using CFT and immunoblot assay. A total of 301 serum samples from horses were collected foe the detection of glanders antibodies from Mymensingh, Tangail and Jamalpur districts in Bangladesh. By CFT 105 samples were found positive and 23 samples were suspicious. The immunoblot confirmed 26 of these samples but 3 remained suspicious. The overall seroprevalence of glanders was 34.9% based on CFT and 24.8% based on immunoblot. Higher prevalence was found in Jamalpur (11.81%). CFT is considered to be a suitable screening test for the diagnosis of glanders in field conditions in Bangladesh. 

REFERENCES

  1. Al-Ani, F.K., Al-Rawashdeh, O.F., Ali, A.H., Hassan, F.K.(1998). Glanders in horses: clinical, biochemical and serological studies in Iraq. Veterinarski Arhives,68:155-162.
  2. Anuntagool, N. and Sirisinha, S.(2002). Antigenic relatedness between Burkholderia pseudomallei and Burkholderia mallei. Microbiology    and immunology,46:143-150.
  3. Bazargani, T., Tadjbakhsh, H., Badii, A., Zahraei, T.(1996). The outbreak of glanders in some racehorses in three states of Iran. Journal of equine veterinary science,16:232-236.
  4. Cravitz, L. and Miller, W.R. (1950). Immunologic studies with Malleomyces mallei and Malleomyces pseudomallei. II. Agglutination and complement fixation tests in man and laboratory animals. The Journal of infectious diseases,86:52-62.
  5. Elschner, M., Klaus, C., Liebler Tenorio, E., Schmoock, G., Wohlsein, P., Tinschmann, O., Lange, E., Kaden, V., Klopfleisch, R., Melzer, F. (2009). Burkholderia mallei infection in a horse imported from Brazil. Equine Veterinary Education,21:147-150.
  6. Elschner, M.C., Scholz, H.C., Melzer, F., Saqib, M., Marten, P., Rassbach, A., Dietzsch, M., Schmoock, G., de Assis Santana, V.L., de Souza, M.M., Wernery, R., Wernery, U., Neubauer, H.(2011). Use of a Western blot technique for the serodiagnosis of glanders.BMC Veterinary Research,19:4.
  7. Katz, J.B., Chieves, L.P., Hennager, S.G., Nicholson, J.M., Fisher, T.A., Byers, P.E. (1999). Serodiagnosis of equine piroplasmosis, dourine, and glanders using an arrayed immunoblotting method. Journal of Veterinary Diagnostic Investigation,11:292-294.
  8. Khan, I., Wieler, L.H., Melzer, F., Elschner, M.C., Muhammad, G., Ali, S., Sprague, L.D., Neubauer, H. and Saqib, M. (2013). Glanders in animals: a review on epidemiology, clinical presentation, diagnosis and countermeasures. Transboundary and emerging diseases, 60:204-221.
  9. Khan, I., Wieler, L.H., Saqib, M., Melzer, F., Santana, V.L., Neubauer, H. and Elschner, M.C. (2014). Effect of incubation temperature on the diagnostic sensitivity of the glanders complement fixation test. Revue Scientifique et Technique, 33:869-875.
  10. Ma, C.(1986). Diagnosis of glanders in horses by the indirect fluorescent antibody (IFA) technique. Chinese Journal of Veterinary Science and Technology,9:3-5.
  11. Marr, J.S. and Malloy, C.D.(1996). An epidemiologic analysis of the ten plagues of Egypt. Caduceus (Springfield, Ill.) 12. pp. 7.
  12. Misra, V. and Arora, A. (1988). Diagnosis of glanders by counter-immuno-electrophoresis test and its efficacy vis-a-vis other test. Journal of Remount and Veterinary Corps, 185.
  13. Mota, R.A., Brito, M.F., Castro, F.J., Massa, M.(2000). Glanders in horses and mules of the states of Pernambuco and Alagoas, Brazil. Pesquisa Veterinária Brasileira,20:155-159.
  14. Muhammad, G., Khan, M.Z., Athar, M. (2001). Clinico-microbiological and therapeutic aspects of glanders in equines. Journal of equine science,9:93-96.
  15. Naureen, A., Saqib, M., Muhammad, G., Hussain, M.H., Asi, M.N.(2007). Comparative evaluation of Rose Bengal plate agglutination test, mallein test, and some conventional serological tests for diagnosis of equine glanders. Journal of veterinary diagnostic investigation,19:362-367.
  16. Neubauer, H., Sprague, L., Zacharia, R., Tomaso, H., Al Dahouk, S., Wernery, R., Wernery, U., Scholz, H.(2005). Serodiagnosis of Burkholderia malleiinfections in horses: State of the art and perspectives. Zoonoses and Public Health,52:201-205.
  17. OIE, (2008). Terrestrial Manual. Chapter2, pp. 11.
  18. Pitt, T., Aucken, H., Dance, D. (1992). Homogeneity of lipopolysaccharide antigens in Pseudomonas pseudomallei. Journal of Infection,25:139-146.
  19. Sprague, L.D., Zachariah, R., Neubauer, H., Wernery, R., Joseph, M., Scholz, H.C., Wernery, U. (2009). Prevalence-dependent use of serological tests for diagnosing glanders in horses. BMC Veterinary Research,5: 32.
  20. Turnbull, A., Wernery, U., Wernery, R., Anandh, J., Kinne, J.(2002). Survey of six infectious diseases of feral donkeys in the United Arab Emirates. Equine Veterinary Education,14: 33-38.
  21. Wilson, G.S. and Miles, A.A. (1946). Principles of Bacteriology and Immunity. Academic Medicine,21: 318.
  22. Zhang, W. and Lu, Z.(1983). Application of an indirect haemagglutination test for the diagnosis of glanders and melioidiosis. Chinese Journal of Veterinary Medicine,9:8-9. 
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