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Current IssueEditorial BoardOnline First ArticlesArchive

Short Communication

volume 53 issue 7 (july 2019) : 856-859,   Doi: 10.18805/ijar.B-904

Analysis of polymorphism of growth hormone secretagogue receptor in goat

J
J.Y. Bai
Y
Y.G . Zhao
G
G.L. Li
Y
Y.B. Yang
Y
Y.Q. Wang
X
X.Wang
S
S.Yang
1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471 003, China.
Cite article:- Bai J.Y., Zhao . Y.G, Li G.L., Yang Y.B., Wang Y.Q., X.Wang, S.Yang (2018). Analysis of polymorphism of growth hormone secretagogue receptor in goat. Indian Journal of Animal Research. 53(7): 856-859. doi: 10.18805/ijar.B-904.

ABSTRACT

Two pairs of primers (GHSR-3, GHSR-4) were used to amplify GHSR gene of Boer goat, Yaoshan goat and black goat. Totally two mutation sites (SNP loci), G200A and T628C were detected in amplification fragments of GHSR-3 and GHSR-4 respectively. For locus G200A, allele frequencies of G and A in Yaoshan goat, Boer goat and black goat were 0.637/0.595/0.827 and 0.363/0.450/0.173, respectively, which indicated that G was the dominant allele in three goat populations. G200A was synonymous mutation. For locus T628C, allele frequencies of C and T in Yaoshan goat, Boer goat and black goat were 0.466/0.449/0.458 and 0.534/0.551/0.542 respectively. Clustering analysis based on GHSR gene sequences of different species showed that Boer goat, Yaoshan goat and black goat were clustered with Capra hircus firstly, then clustered with Ovis aries and Pantholops hodgsonii, and finally clustered with cattle and whale. 

REFERENCES

  1. Bai,J.Y., Jia,X.P., Yang,Y.B., Pang,Y.Z., Qi, Y.X. (2016a). Polymorphism Analysis of LHâ gene in sheep. Pakistan J. Zool.,48(4):931-    935.
  2. Bai,J.Y., Wang,X., Yang,Y.B., Zhang,X.H., Pang,Y.Z., Li,H.W. (2016b). Study on the polymorphism of POU1F1 gene in sheep. R. Bras. Zootec., 45(10):604-607.
  3. Huang,A.Z., Song,T.W.,Luo,W.X., Sun,Y.Y.,Liu,B.,Song,Q.W.,Yang,Y.(2014).Genetic variations of GHSR gene and its correlation with growth character in goat. Journal of Mountain Agriculture and Biology, 33(5):43-48. (In Chinese)
  4. Kitazawa,T.,Hiraga,T.,Teraoka,H.,Yaosakaa,N., Kaiyac,H.(2014). Correlation of ghrelin concentration and ghrelin, ghrelin-O-    acetyltransferase (GOAT) and growth hormone secretagogue receptor 1a mRNAs expression in the proventriculus and brain of the growing chicken. Peptides, 63:134-142.
  5. Li,D.,Song,T.W.,Luo,W.X.,Wei,G.Q.,Zhao,Y.(2013).Polymorphism of Exon of GHSR Gene and relevance analysis with body weight and size in Qianbei Ma Goat. Guizhou Agricultural Sciences, 41(11):122-125. (In Chinese)
  6. Liu,B.,Song,T.W.,Luo,W.X.,Cai,H.F., Sun,Y.Y.(2013). Study on relationship between polymorphism of GHSR gene and growth traits in Qianbei Ma goat. Guangdong Agricultural Sciences, 22:161. (In Chinese)
  7. Song,T.W.,Cai,H.F.,Luo,W.X.,Liu,R.Y.,Zhang,Y.Y.,Sun,Y.Y.,Liu,B.(2015).Association of GHSR and GHRL gene Genetic Variation with Growth Traits in Two Guizhou Goat Breeds. Scientia Agricultura Sinica,48(1):140-153
  8. Song,T.W.,Luo,W.X.,Cai,H.F.,Liu,B.,Sun,Y.Y.(2013).Polymorphism of GHSR gene and its relationship with body eight and body size traits in goat. Acta Veterinaria Et Zootechnica Sinica, 44(11): 1717-1723. (In Chinese)
  9. Yu,G.S.,Zhang,W.L.,Huang,Y.,Zhao,X.M.,Li,W.,Dong,D.W.(2004).Expression and distribution of GHSR-1a in the gastrointestinal tract of dairy goat. Chinese Journal of Veterinary Science, 105-109,115. (In Chinese)
  10. Zhang,N.,Ding.Y.P.,Wang.J.L.,Shao,B.P.(2015).Ghrelin and Energy Metabolism. Chinese Journal Of Animal Nutrition, 27( 8) :2349-    2360. (In Chinese)
  11. Zhang,Q,D.,Song,D.R.,Zhou,D.R.,Peng,H.,Liu,N.Y.,Wu,R.R.,Jiang,X.M.,Wu,P.(2014).Screening the GHSR gene for SNPs and analyzing the mutations in Guizhou Semi-fine wool sheep. China Animal Husbandry & Veterinary Medicine, 41(4):189-    193. (In Chinese)
  12. Zhu,G.Q.,Luo,W.X.,Wu,X.,Han,Y.,Kong,D.S.,Song,T.W.(2014).Study on the GHSR gene Mutation and its relationships with body Weight and body Size traits in Guizhou Black Goats. Chinese Journal of Animal Science, 50(9):23-27. (In Chinese)
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Indian Journal of Animal Research
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    Short Communication

    volume 53 issue 7 (july 2019) : 856-859,   Doi: 10.18805/ijar.B-904

    Analysis of polymorphism of growth hormone secretagogue receptor in goat

    J
    J.Y. Bai
    Y
    Y.G . Zhao
    G
    G.L. Li
    Y
    Y.B. Yang
    Y
    Y.Q. Wang
    X
    X.Wang
    S
    S.Yang
    1College of Animal Science and Technology, Henan University of Science and Technology, Luoyang 471 003, China.
    Cite article:- Bai J.Y., Zhao . Y.G, Li G.L., Yang Y.B., Wang Y.Q., X.Wang, S.Yang (2018). Analysis of polymorphism of growth hormone secretagogue receptor in goat. Indian Journal of Animal Research. 53(7): 856-859. doi: 10.18805/ijar.B-904.

    ABSTRACT

    Two pairs of primers (GHSR-3, GHSR-4) were used to amplify GHSR gene of Boer goat, Yaoshan goat and black goat. Totally two mutation sites (SNP loci), G200A and T628C were detected in amplification fragments of GHSR-3 and GHSR-4 respectively. For locus G200A, allele frequencies of G and A in Yaoshan goat, Boer goat and black goat were 0.637/0.595/0.827 and 0.363/0.450/0.173, respectively, which indicated that G was the dominant allele in three goat populations. G200A was synonymous mutation. For locus T628C, allele frequencies of C and T in Yaoshan goat, Boer goat and black goat were 0.466/0.449/0.458 and 0.534/0.551/0.542 respectively. Clustering analysis based on GHSR gene sequences of different species showed that Boer goat, Yaoshan goat and black goat were clustered with Capra hircus firstly, then clustered with Ovis aries and Pantholops hodgsonii, and finally clustered with cattle and whale. 

    REFERENCES

    1. Bai,J.Y., Jia,X.P., Yang,Y.B., Pang,Y.Z., Qi, Y.X. (2016a). Polymorphism Analysis of LHâ gene in sheep. Pakistan J. Zool.,48(4):931-    935.
    2. Bai,J.Y., Wang,X., Yang,Y.B., Zhang,X.H., Pang,Y.Z., Li,H.W. (2016b). Study on the polymorphism of POU1F1 gene in sheep. R. Bras. Zootec., 45(10):604-607.
    3. Huang,A.Z., Song,T.W.,Luo,W.X., Sun,Y.Y.,Liu,B.,Song,Q.W.,Yang,Y.(2014).Genetic variations of GHSR gene and its correlation with growth character in goat. Journal of Mountain Agriculture and Biology, 33(5):43-48. (In Chinese)
    4. Kitazawa,T.,Hiraga,T.,Teraoka,H.,Yaosakaa,N., Kaiyac,H.(2014). Correlation of ghrelin concentration and ghrelin, ghrelin-O-    acetyltransferase (GOAT) and growth hormone secretagogue receptor 1a mRNAs expression in the proventriculus and brain of the growing chicken. Peptides, 63:134-142.
    5. Li,D.,Song,T.W.,Luo,W.X.,Wei,G.Q.,Zhao,Y.(2013).Polymorphism of Exon of GHSR Gene and relevance analysis with body weight and size in Qianbei Ma Goat. Guizhou Agricultural Sciences, 41(11):122-125. (In Chinese)
    6. Liu,B.,Song,T.W.,Luo,W.X.,Cai,H.F., Sun,Y.Y.(2013). Study on relationship between polymorphism of GHSR gene and growth traits in Qianbei Ma goat. Guangdong Agricultural Sciences, 22:161. (In Chinese)
    7. Song,T.W.,Cai,H.F.,Luo,W.X.,Liu,R.Y.,Zhang,Y.Y.,Sun,Y.Y.,Liu,B.(2015).Association of GHSR and GHRL gene Genetic Variation with Growth Traits in Two Guizhou Goat Breeds. Scientia Agricultura Sinica,48(1):140-153
    8. Song,T.W.,Luo,W.X.,Cai,H.F.,Liu,B.,Sun,Y.Y.(2013).Polymorphism of GHSR gene and its relationship with body eight and body size traits in goat. Acta Veterinaria Et Zootechnica Sinica, 44(11): 1717-1723. (In Chinese)
    9. Yu,G.S.,Zhang,W.L.,Huang,Y.,Zhao,X.M.,Li,W.,Dong,D.W.(2004).Expression and distribution of GHSR-1a in the gastrointestinal tract of dairy goat. Chinese Journal of Veterinary Science, 105-109,115. (In Chinese)
    10. Zhang,N.,Ding.Y.P.,Wang.J.L.,Shao,B.P.(2015).Ghrelin and Energy Metabolism. Chinese Journal Of Animal Nutrition, 27( 8) :2349-    2360. (In Chinese)
    11. Zhang,Q,D.,Song,D.R.,Zhou,D.R.,Peng,H.,Liu,N.Y.,Wu,R.R.,Jiang,X.M.,Wu,P.(2014).Screening the GHSR gene for SNPs and analyzing the mutations in Guizhou Semi-fine wool sheep. China Animal Husbandry & Veterinary Medicine, 41(4):189-    193. (In Chinese)
    12. Zhu,G.Q.,Luo,W.X.,Wu,X.,Han,Y.,Kong,D.S.,Song,T.W.(2014).Study on the GHSR gene Mutation and its relationships with body Weight and body Size traits in Guizhou Black Goats. Chinese Journal of Animal Science, 50(9):23-27. (In Chinese)
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