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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 12 (december 2018) : 1688-1694,   Doi: 10.18805/ijar.B-901

Activity, acute and Sub-acute toxicity and safety assesment of the hydroalcholic root extract of Diplotaenia turcica

U
U. Özdek
Y
Y. Baþbuðan
S
S. Yýldýrým
M
M. Boða
M
M. Fýrat
Y
Y. Deðer
1Van Yuzuncu Yil University, Vocational School of Health Services, 65080 Van, Turkey.
Cite article:- Özdek U., Baþbuðan Y., Yýldýrým S., Boða M., Fýrat M., Deðer Y. (2018). Activity, acute and Sub-acute toxicity and safety assesment of the hydroalcholic root extract of Diplotaenia turcica. Indian Journal of Animal Research. 52(12): 1688-1694. doi: 10.18805/ijar.B-901.

ABSTRACT

Diplotaenia  turcica is widely utilized in conventional treatment in the east of Turkey. Due to the insufficient data on the safety profile, the acute and  sub acute toxicity of Diplotaenia turcica was determined. Furthermore, total phenolic content, flavonoid content and antioxidant activity of the extract were determined. The acute toxicity of hydroalcoholic root extract of Diplotaenia turcica (HREDT) was evaluated in mice after administration of single oral dose at the 5000 mg/kg (acute model) and rat after 28 days orally administration at the dose range of 250, 500 and 1000 mg/kg (subacute model). According to result, the LD50 value of HREDT was found to be greater than 5000 mg/kg. In sub acute toxicity study, no statistically significant differences were observed in the values of hematological and pathological parameters in comparison with control group. It was determined that the glucose, triglyceride, cholesterol and LDL levels exerted a significant effect depending on the HREDT doses. The results from the present study found out that HREDT did not produce any toxic effects or deaths in animals for both single and chronic administration. Additionally, HREDT showed moderate antioxidant activities and exhibited relatively notable total phenolic content.

REFERENCES

  1. Alam M.N, Bristi N.J., and Rafiquzzaman M. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21: 143-152.
  2. Apak R., Güçlü K., Özyürek M., and Karademir S.E.(2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52: 7970-7981.
  3. Arýtuluk Z.C., Tatlý Çankaya Ý.Ý., and Gençler Özkan A.M. (2016). Antioxidant activity, total phenolic and flavonoid contents of some tanacetum l. (asteraceae) taxa growing in Turkey. FABAD Journal of Pharmaceutical Sciences, 41: 17-25.
  4. Curtis D. (2007). Principle of Toxicology. In: Eaton D, Gilbert S, editors. Casaretts Doull’s Toxicology. Mc Graw Hill;. pp. 7–14.
  5. El Shahat A, El-Shennawy H ,El-Megid MHA (2017). Studying the protective effect of gamma-irradiated basil (Ocimum basilicum L.) against methotrexate-induced liver and renal toxicity in rats. Indian Journal of Animal Research, 51: 1, 135-140.
  6. Farkhad N.K., Farokhi F., and Tukmacki A. (2012). Hydro-alcoholic extract of the root of Prangos ferulacea (L.) Lindl can improve serum glucose and lipids in alloxan-induced diabetic rats. Avicenna Journal of Phytomedicine, 2: 179-187.
  7. Fýrat M. (2013). Dictionary of Plant Names in Kurdish. Ankara-Turkey: KalkanOfset.
  8. Kaval I., Behçet L., and Cakilcioglu U. (2014). Ethnobotanical study on medicinal plants in Geçitli and its surrounding (Hakkari-    Turkey). Journal of Ethnopharmacology, 155: 171-184.
  9. Krithika V, Naik R (2015). Functional properties of grape (Vitis vinifera) seed extract and possible extraction techniques-A review. Agricultural Reviews, 36: 4.
  10. Kosanic M., Rankovic B., and Dašic M. (2012). Mushrooms as possible antioxidant and antimicrobial agents. Iranian Journal of Pharmaceutical Research, 11: 1095-1102.
  11. Lohith T, Shridhar N, Jayakumar K, Sathyanarayana M, Dilip S ,Gowda KH (2013). Acute oral toxicity of raw areca nut extract in rats. Indian Journal of Animal Research, 47: 5, 431-433.
  12. Luna Lee G.(1968). Manual of Histologic Staining Methods of the Armed Forces Institute of pathology, New York.
  13. Moreno M.I., Isla M.I., Sampietro A.R., and Vattuone M.A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology, 71: 109-114.
  14. Pimenov M., Kljuykov E., and Degtjareva G. (2011). Survey of the genus Diplotaenia (Umbelliferae), with description of two new species from Turkey. Willdenowia, 41: 67-74.
  15. Prasanth K.M., Suba V., Ramireddy B., and Srinivasa B.P. (2015). Acute and subchronic oral toxicity assessment of the ethanolic extract of the root of Oncoba spinosa (Flacourtiaceae) in rodents. Tropical Journal of Pharmaceutical Research, 14: 1849-1855.
  16. Shahidi F. and Zhong Y. (2015). Measurement of antioxidant activity. Journal of Functional Foods, 18, 757-781.
  17. Slinkard K. and Singleton V.L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.
  18. Taylor C. R. and Cote R. J. (2006). Immunomicroscopy: a diagnostic tool for the surgical pathologist, WB Saunders Company.
  19. Uce Ý. and Tunçtürk M. (2014). Wild plants which naturally grown and widely used in Hakkari. Biyoloji Bilimleri Araþtýrma Dergisi, 7: 21-25.
  20. Yaqub A, Anjum KM, Munir A, Mukhtar H ,Khan WA (2018). Evaluation of acute toxicity and effects of sub-acute concentrations of copper oxide nanoparticles (CuO-NPs) on hematology, selected enzymes and histopathology of liver and kidney in Mus musculus. Indian Journal of Animal Research, 52: 1, 92-98. 
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 12 (december 2018) : 1688-1694,   Doi: 10.18805/ijar.B-901

    Activity, acute and Sub-acute toxicity and safety assesment of the hydroalcholic root extract of Diplotaenia turcica

    U
    U. Özdek
    Y
    Y. Baþbuðan
    S
    S. Yýldýrým
    M
    M. Boða
    M
    M. Fýrat
    Y
    Y. Deðer
    1Van Yuzuncu Yil University, Vocational School of Health Services, 65080 Van, Turkey.
    Cite article:- Özdek U., Baþbuðan Y., Yýldýrým S., Boða M., Fýrat M., Deðer Y. (2018). Activity, acute and Sub-acute toxicity and safety assesment of the hydroalcholic root extract of Diplotaenia turcica. Indian Journal of Animal Research. 52(12): 1688-1694. doi: 10.18805/ijar.B-901.

    ABSTRACT

    Diplotaenia  turcica is widely utilized in conventional treatment in the east of Turkey. Due to the insufficient data on the safety profile, the acute and  sub acute toxicity of Diplotaenia turcica was determined. Furthermore, total phenolic content, flavonoid content and antioxidant activity of the extract were determined. The acute toxicity of hydroalcoholic root extract of Diplotaenia turcica (HREDT) was evaluated in mice after administration of single oral dose at the 5000 mg/kg (acute model) and rat after 28 days orally administration at the dose range of 250, 500 and 1000 mg/kg (subacute model). According to result, the LD50 value of HREDT was found to be greater than 5000 mg/kg. In sub acute toxicity study, no statistically significant differences were observed in the values of hematological and pathological parameters in comparison with control group. It was determined that the glucose, triglyceride, cholesterol and LDL levels exerted a significant effect depending on the HREDT doses. The results from the present study found out that HREDT did not produce any toxic effects or deaths in animals for both single and chronic administration. Additionally, HREDT showed moderate antioxidant activities and exhibited relatively notable total phenolic content.

    REFERENCES

    1. Alam M.N, Bristi N.J., and Rafiquzzaman M. (2013). Review on in vivo and in vitro methods evaluation of antioxidant activity. Saudi Pharmaceutical Journal, 21: 143-152.
    2. Apak R., Güçlü K., Özyürek M., and Karademir S.E.(2004). Novel total antioxidant capacity index for dietary polyphenols and vitamins C and E, using their cupric ion reducing capability in the presence of neocuproine: CUPRAC method. Journal of Agricultural and Food Chemistry, 52: 7970-7981.
    3. Arýtuluk Z.C., Tatlý Çankaya Ý.Ý., and Gençler Özkan A.M. (2016). Antioxidant activity, total phenolic and flavonoid contents of some tanacetum l. (asteraceae) taxa growing in Turkey. FABAD Journal of Pharmaceutical Sciences, 41: 17-25.
    4. Curtis D. (2007). Principle of Toxicology. In: Eaton D, Gilbert S, editors. Casaretts Doull’s Toxicology. Mc Graw Hill;. pp. 7–14.
    5. El Shahat A, El-Shennawy H ,El-Megid MHA (2017). Studying the protective effect of gamma-irradiated basil (Ocimum basilicum L.) against methotrexate-induced liver and renal toxicity in rats. Indian Journal of Animal Research, 51: 1, 135-140.
    6. Farkhad N.K., Farokhi F., and Tukmacki A. (2012). Hydro-alcoholic extract of the root of Prangos ferulacea (L.) Lindl can improve serum glucose and lipids in alloxan-induced diabetic rats. Avicenna Journal of Phytomedicine, 2: 179-187.
    7. Fýrat M. (2013). Dictionary of Plant Names in Kurdish. Ankara-Turkey: KalkanOfset.
    8. Kaval I., Behçet L., and Cakilcioglu U. (2014). Ethnobotanical study on medicinal plants in Geçitli and its surrounding (Hakkari-    Turkey). Journal of Ethnopharmacology, 155: 171-184.
    9. Krithika V, Naik R (2015). Functional properties of grape (Vitis vinifera) seed extract and possible extraction techniques-A review. Agricultural Reviews, 36: 4.
    10. Kosanic M., Rankovic B., and Dašic M. (2012). Mushrooms as possible antioxidant and antimicrobial agents. Iranian Journal of Pharmaceutical Research, 11: 1095-1102.
    11. Lohith T, Shridhar N, Jayakumar K, Sathyanarayana M, Dilip S ,Gowda KH (2013). Acute oral toxicity of raw areca nut extract in rats. Indian Journal of Animal Research, 47: 5, 431-433.
    12. Luna Lee G.(1968). Manual of Histologic Staining Methods of the Armed Forces Institute of pathology, New York.
    13. Moreno M.I., Isla M.I., Sampietro A.R., and Vattuone M.A. (2000). Comparison of the free radical-scavenging activity of propolis from several regions of Argentina. Journal of ethnopharmacology, 71: 109-114.
    14. Pimenov M., Kljuykov E., and Degtjareva G. (2011). Survey of the genus Diplotaenia (Umbelliferae), with description of two new species from Turkey. Willdenowia, 41: 67-74.
    15. Prasanth K.M., Suba V., Ramireddy B., and Srinivasa B.P. (2015). Acute and subchronic oral toxicity assessment of the ethanolic extract of the root of Oncoba spinosa (Flacourtiaceae) in rodents. Tropical Journal of Pharmaceutical Research, 14: 1849-1855.
    16. Shahidi F. and Zhong Y. (2015). Measurement of antioxidant activity. Journal of Functional Foods, 18, 757-781.
    17. Slinkard K. and Singleton V.L. (1977). Total phenol analysis: automation and comparison with manual methods. American Journal of Enology and Viticulture, 28: 49-55.
    18. Taylor C. R. and Cote R. J. (2006). Immunomicroscopy: a diagnostic tool for the surgical pathologist, WB Saunders Company.
    19. Uce Ý. and Tunçtürk M. (2014). Wild plants which naturally grown and widely used in Hakkari. Biyoloji Bilimleri Araþtýrma Dergisi, 7: 21-25.
    20. Yaqub A, Anjum KM, Munir A, Mukhtar H ,Khan WA (2018). Evaluation of acute toxicity and effects of sub-acute concentrations of copper oxide nanoparticles (CuO-NPs) on hematology, selected enzymes and histopathology of liver and kidney in Mus musculus. Indian Journal of Animal Research, 52: 1, 92-98. 
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