Expression and characterization of a lysine rich protein in cow milk

DOI: 10.18805/ijar.B-882    | Article Id: B-882 | Page : 874-879
Citation :- Expression and characterization of a lysine rich protein in cow milk.Indian Journal Of Animal Research.2019.(53):874-879
Xin Ma, Li Su, Peng Zhang, Sheng Zhang, Bo Tang, Xueming Zhang, Weimin Luan and Ziyi Li
Address : College of Animal Science and Technology, Jilin Agricultural University, Changchun, China.
Submitted Date : 13-12-2017
Accepted Date : 3-04-2018


The lysine is considered as the most important essential amino acid, because it is the most limiting in the cereals grains. In this study, a lysine-rich (LR) gene, and the expression vector pcDNA3.1-LR and pBC1-LR were constructed. The LR was expressed in 293T cells driven by the vector pcDNA3.1-LR and checked by RT-PCR and WB. The mammary gland tissue-specific expression vector carrying the LR was injected directly into the lactating mammary glands of cows and the milk samples were checked by a complete amino acid analysis. The results showed that the LR protein was expressed successfully in cells and in cow milk; the expression of LR lasted for 6 d, and the lysine level of the injection group was significantly higher than that of negative controls (p <0.05). This study provide a better understanding of how mammary gland expression systems increase the lysine content of milk that can be applied to transgenic dairy cow.


Cow milk Lysine rich gene expression 293T Cells


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