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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 2 (february 2019) : 146-150,   Doi: 10.18805/ijar.B-873

Determination of the genetic polymorphism for DGAT1 gene in Holstein, Jersey and native cattle breeds of Turkey

E
E.K. Gurcan
O
O. Cobanoglu
E
E. Kul
H
H.S. Abaci
S
S. Cankaya
1Department of Animal Science, Faculty of Agriculture, Namik Kemal University, Tekirdag 59030, Turkey
Cite article:- Gurcan E.K., Cobanoglu O., Kul E., Abaci H.S., Cankaya S. (2018). Determination of the genetic polymorphism for DGAT1 gene in Holstein, Jersey and native cattle breeds of Turkey. Indian Journal of Animal Research. 53(2): 146-150. doi: 10.18805/ijar.B-873.

ABSTRACT

This study was to determine the genetic polymorphism in terms of the DGAT1 gene among Holstein and Jersey and indigenous Native Black, East Anatolian Red and Grey Steppe in Turkey. The frequencies of K allele for the DGAT1 gene were calculated as 0.59, 0.54, 0.53, 0.62, 0.52, and 0.54 for Jersey, Holstein (Black Sea Region), Holstein (Marmara Region), and Grey Steppe, East Anatolian Red, and Native Black breeds, respectively. The FIS values of the populations for the DGAT1 gene were detected all negative values. In overall population, FIS value was found as 76%.  The expected deviations from the Hardy-Weinberg Equilibrium in terms of the DGAT1 locus were found significant in these six different populations (P<0.01). The genetic distance values among the populations were calculated between 0.0001 and 0.0089. Based on the cluster analysis, Holstein, Native Black and East Anatolian Red were located in close cluster; however, Jersey and Grey Steppe were grouped in different cluster. 

REFERENCES

  1. Cerit, H. and Dümen, E. (2015). Identification of K232A mutant allele of DGAT1 gene encoding diacylglycerol 1 Acyltransferase enzyme. Journal of Faculty of Veterinary Medicine, Erciyes University Research Article 12 (3): 171-176. 
  2. Devrim, A. K. and Kaya, N. (2006). An investigation on DNA polymorphism of the cattle breeds in the province of Kars by RAPD-    PCR technique. Revue Med. Vet., 157 (2): 88-91.
  3. Komisarek, J., Waœkowicz, K., Michalak, A. and Dorynek, Z. (2004). Effects of DGAT1 variants on milk Production traits in Jersey cattle. Institute of Genetics and Animal Breeding, Jastrzêbiec, Poland. Animal Science Papers and Reports. 22: (3) 307-313.
  4. Kimura, M and Crow, JM (1964). Effect of overall phenotypic selection on genetic change at individual loci. Proc. Natnl. Acad. Sci., Washington. 75: 6168-6171.
  5. Kwon, J. M. and Goate, A. M. (2000). The candidate gene approach. Alcohol Res Health. 24 (3): 164-8. 
  6. Levene, H. (1949). On a matching problem arising in genetics. Ann. Math. Stat. 20: 91-94.
  7. Mashhadi, M.H., Nassiri, MR., Eman, N., Kashan, J. and Torzhizi, R. V. (2013). Association between DGAT1 genotype and breeding value of milk Production traits in Iranian Holstein bulls. Iranian Journal of Applied Animal Science. 3 (4): 813-815.
  8. Mashhadi, M.H., Nassiri, MR., Mahmoudi, M., Rastin, M., Kashan, J., Torzhizi, R. V., Tabasiandse, N. and Noorave, S. E. (2012). Polymorphism and sequencing of DGAT1 gene in Iranian Holstein bulls. Iranian Journal of Applied Animal Science. 2 (1): 63-67.
  9. Mohammed, A.S., Rahamtalla, A.S., Ahmed, S.S., Elamin, K. M., Douse, B.M., Elhafiz, A., Ahmed, M.K.A (2015). DGAT1 Gene in Dairy Cattle: A Review. Global Journal of Animal Scientific Research. 3 (1): 191-198.
  10. Nei, M. (1972). Genetic distance between populations. The American Naturalist 106, No. 949 (May - Jun., 1972), pp. 283-292
  11. Nei, M. and Weh-Hsiung L. (1973) Linkage disequilibrium in subdivided populations. Genetics, 75 (1): 2133-219. 
  12. Nei, M. (1987). Molecular Evolutionary Genetics, Columbia University Press, New York.
  13. Nowacka-Woszuk, J; Noskowiak, A; Strabel, T; Jankowski, T and Switonski, M (2008). An effect of the DGAT1 gene polymorphism on breeding value of Polish Holstein- Friesian sires. Animal Science papers and Reports, 26 (1): 17-23.
  14. Sambrook, J., Russel, D. W. and Sambrook, J. (2001). Molecular Cloning: a Laboratory Manual. 3rd edn. Cold Spring Harbor Laboratory, New York, NY, USA, 999.
  15. Sanders, K; Bennewitz,J; Reinsch, N; Thaller, G; Prinzenberg, E.M; Kühn, C and Kalm, E (2006). Characterization of the DGAT1 mutations and the CSN1S1 promoter in the German Angeln dairy cattle population. J. Dairy Sci., 89: 3164–3174.
  16. Scotti, E., Fontanesi, L., Schiavini, F., La Mattina, V., Bagnota, A. and Russo, V. (2010). ‘’DGAT1 p.K232A polymorphism in dairy and dual Italian cattle breeds’’, Italian Journal of Animal Science, 9: 16.
  17. Soller, M., Brody, T. and Genizi, A. (1976). On the power of experimental designs for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theoretical and Applied Genetics, 47,35.
  18. Thaller, G., Kramer, W., Winter, A., Kaupe, B. and Erhardt, G. (2003). Effects of DGAT1 variants on milk production traits in German cattle breeds. J. Anim. Sci. 81: 1911–1918.
  19. Yeh, F., Yang, R. C., Boyle, T. (2000). PopGene(v.1.32), Microsoft Windows-based freeware for population genetic analysis. Retrieved from http://www.ualberta.ca/fyeh/Pop32.exe
  20. Wright, S. (1969). Evolution and the genetics of population. Vol. 2. The theory of gene frequencies. Univ. of Chicago Press, Chicago, IL.
  21. Wright, S. (1978). The interpretation of populations’ structure by F-statistics with special regard to system of mating. Evolution 19: 395-420. 
  22. Winter, A., Kramer, W., Werner, F. A. O., Kollers, S., Kata, S., Durstewitz, G., Buitkamp, J., Womack, J. E., Thaller, G. and Fries R. (2002). Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc. Natl. Acad. Sci., USA 16: 9252–9257. 
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    Research Article

    volume 53 issue 2 (february 2019) : 146-150,   Doi: 10.18805/ijar.B-873

    Determination of the genetic polymorphism for DGAT1 gene in Holstein, Jersey and native cattle breeds of Turkey

    E
    E.K. Gurcan
    O
    O. Cobanoglu
    E
    E. Kul
    H
    H.S. Abaci
    S
    S. Cankaya
    1Department of Animal Science, Faculty of Agriculture, Namik Kemal University, Tekirdag 59030, Turkey
    Cite article:- Gurcan E.K., Cobanoglu O., Kul E., Abaci H.S., Cankaya S. (2018). Determination of the genetic polymorphism for DGAT1 gene in Holstein, Jersey and native cattle breeds of Turkey. Indian Journal of Animal Research. 53(2): 146-150. doi: 10.18805/ijar.B-873.

    ABSTRACT

    This study was to determine the genetic polymorphism in terms of the DGAT1 gene among Holstein and Jersey and indigenous Native Black, East Anatolian Red and Grey Steppe in Turkey. The frequencies of K allele for the DGAT1 gene were calculated as 0.59, 0.54, 0.53, 0.62, 0.52, and 0.54 for Jersey, Holstein (Black Sea Region), Holstein (Marmara Region), and Grey Steppe, East Anatolian Red, and Native Black breeds, respectively. The FIS values of the populations for the DGAT1 gene were detected all negative values. In overall population, FIS value was found as 76%.  The expected deviations from the Hardy-Weinberg Equilibrium in terms of the DGAT1 locus were found significant in these six different populations (P<0.01). The genetic distance values among the populations were calculated between 0.0001 and 0.0089. Based on the cluster analysis, Holstein, Native Black and East Anatolian Red were located in close cluster; however, Jersey and Grey Steppe were grouped in different cluster. 

    REFERENCES

    1. Cerit, H. and Dümen, E. (2015). Identification of K232A mutant allele of DGAT1 gene encoding diacylglycerol 1 Acyltransferase enzyme. Journal of Faculty of Veterinary Medicine, Erciyes University Research Article 12 (3): 171-176. 
    2. Devrim, A. K. and Kaya, N. (2006). An investigation on DNA polymorphism of the cattle breeds in the province of Kars by RAPD-    PCR technique. Revue Med. Vet., 157 (2): 88-91.
    3. Komisarek, J., Waœkowicz, K., Michalak, A. and Dorynek, Z. (2004). Effects of DGAT1 variants on milk Production traits in Jersey cattle. Institute of Genetics and Animal Breeding, Jastrzêbiec, Poland. Animal Science Papers and Reports. 22: (3) 307-313.
    4. Kimura, M and Crow, JM (1964). Effect of overall phenotypic selection on genetic change at individual loci. Proc. Natnl. Acad. Sci., Washington. 75: 6168-6171.
    5. Kwon, J. M. and Goate, A. M. (2000). The candidate gene approach. Alcohol Res Health. 24 (3): 164-8. 
    6. Levene, H. (1949). On a matching problem arising in genetics. Ann. Math. Stat. 20: 91-94.
    7. Mashhadi, M.H., Nassiri, MR., Eman, N., Kashan, J. and Torzhizi, R. V. (2013). Association between DGAT1 genotype and breeding value of milk Production traits in Iranian Holstein bulls. Iranian Journal of Applied Animal Science. 3 (4): 813-815.
    8. Mashhadi, M.H., Nassiri, MR., Mahmoudi, M., Rastin, M., Kashan, J., Torzhizi, R. V., Tabasiandse, N. and Noorave, S. E. (2012). Polymorphism and sequencing of DGAT1 gene in Iranian Holstein bulls. Iranian Journal of Applied Animal Science. 2 (1): 63-67.
    9. Mohammed, A.S., Rahamtalla, A.S., Ahmed, S.S., Elamin, K. M., Douse, B.M., Elhafiz, A., Ahmed, M.K.A (2015). DGAT1 Gene in Dairy Cattle: A Review. Global Journal of Animal Scientific Research. 3 (1): 191-198.
    10. Nei, M. (1972). Genetic distance between populations. The American Naturalist 106, No. 949 (May - Jun., 1972), pp. 283-292
    11. Nei, M. and Weh-Hsiung L. (1973) Linkage disequilibrium in subdivided populations. Genetics, 75 (1): 2133-219. 
    12. Nei, M. (1987). Molecular Evolutionary Genetics, Columbia University Press, New York.
    13. Nowacka-Woszuk, J; Noskowiak, A; Strabel, T; Jankowski, T and Switonski, M (2008). An effect of the DGAT1 gene polymorphism on breeding value of Polish Holstein- Friesian sires. Animal Science papers and Reports, 26 (1): 17-23.
    14. Sambrook, J., Russel, D. W. and Sambrook, J. (2001). Molecular Cloning: a Laboratory Manual. 3rd edn. Cold Spring Harbor Laboratory, New York, NY, USA, 999.
    15. Sanders, K; Bennewitz,J; Reinsch, N; Thaller, G; Prinzenberg, E.M; Kühn, C and Kalm, E (2006). Characterization of the DGAT1 mutations and the CSN1S1 promoter in the German Angeln dairy cattle population. J. Dairy Sci., 89: 3164–3174.
    16. Scotti, E., Fontanesi, L., Schiavini, F., La Mattina, V., Bagnota, A. and Russo, V. (2010). ‘’DGAT1 p.K232A polymorphism in dairy and dual Italian cattle breeds’’, Italian Journal of Animal Science, 9: 16.
    17. Soller, M., Brody, T. and Genizi, A. (1976). On the power of experimental designs for the detection of linkage between marker loci and quantitative loci in crosses between inbred lines. Theoretical and Applied Genetics, 47,35.
    18. Thaller, G., Kramer, W., Winter, A., Kaupe, B. and Erhardt, G. (2003). Effects of DGAT1 variants on milk production traits in German cattle breeds. J. Anim. Sci. 81: 1911–1918.
    19. Yeh, F., Yang, R. C., Boyle, T. (2000). PopGene(v.1.32), Microsoft Windows-based freeware for population genetic analysis. Retrieved from http://www.ualberta.ca/fyeh/Pop32.exe
    20. Wright, S. (1969). Evolution and the genetics of population. Vol. 2. The theory of gene frequencies. Univ. of Chicago Press, Chicago, IL.
    21. Wright, S. (1978). The interpretation of populations’ structure by F-statistics with special regard to system of mating. Evolution 19: 395-420. 
    22. Winter, A., Kramer, W., Werner, F. A. O., Kollers, S., Kata, S., Durstewitz, G., Buitkamp, J., Womack, J. E., Thaller, G. and Fries R. (2002). Association of a lysine-232/alanine polymorphism in a bovine gene encoding acyl-CoA:diacylglycerol acyltransferase (DGAT1) with variation at a quantitative trait locus for milk fat content. Proc. Natl. Acad. Sci., USA 16: 9252–9257. 
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