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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 2 (february 2019) : 187-191,   Doi: 10.18805/ijar.B-862

Mitochondrial cytochrome oxidase subunit I-sequence variation in three scorpion species from southeast Turkey 

S
Sahin Toprak
A
Arif Parmaksiz
N
Nevin Aslan
1Harran University, Faculty of Science and Art, Department of Biology, Sanliurfa - 63100, Turkey.
Cite article:- Toprak Sahin, Parmaksiz Arif, Aslan Nevin (2018). Mitochondrial cytochrome oxidase subunit I-sequence variation in three scorpion species from southeast Turkey. Indian Journal of Animal Research. 53(2): 187-191. doi: 10.18805/ijar.B-862.

ABSTRACT

Southeastern Anatolia is the richest region in Turkey in terms of scorpion fauna. In this region, a number of studies on the fauna of scorpions, as well as epidemiology and clinical findings of scorpion stings in terms of public health, have been carried out, but there is no study on the phylogenetic relationships between species. In this study, 18 individuals of three species were collected from five locations. Sequence analysis was conducted by amplification of a 546 base region from part of mtDNA COI region. Six polymorphic regions and four haplotypes were determined for A. crassicauda, a species important for humans and mean haplotype and nucleotide variations were 0.602 and 0.00555 respectively. Sequences from all individuals were subjected to the blast tool of the NCBI database and assessed with close species, so a phylogenetic tree was formed. On the tree, samples from Þanlýurfa and Diyarbakýr were on different branches while the sample from Iran (HM567333) was on a branch from the other two. The species that was the closest to Scorpio maurus from Batman (KF953944) was the sample from Diyarbakýr. Because there was no other sequence for Buthacus macrocentrus, it was located close to the Buthacus sp. (KF548116) sample. As a result, the locations of sample species were determined as a result of phylogenetic analyses. 

REFERENCES

  1. Crucitti, P., and V. Vignoli (2002). Gli Scorpioni (Scorpiones) dell’Anatolia sud-orientale (Turchia). Boll. Mus. Sci. Nat. Torino, 19(2), 433-474.
  2. Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299. 
  3. Karatas, A., and Çolak, M. (2005). Scorpions of Gaziantep Province, Turkey (Arachnida: Scorpiones). Euscorpius, 30: 1-7.
  4. Kumar, S., Stecher, G. and Tamura, K. (2016). Molecular evolutionary genetics analysis version 7.0 (MEGA 7) for bigger datasets. Molecular Biology and Evolution. 28: 2731-2739. 
  5. Kürtüllü, M. (2006). An investigation of the scorpion species (ordo: Scorpiones) of Mardin province. MSc. Thesis. Nigde University, Nigde, 51p.
  6. Ozkan, O., Ahmet, C. and Zafer, K. (2010). A study on the genetic diversity of Androctonus crassicauda (Olivier, 1807; Scorpiones: Buthidae) from Turkey. Journal of Venomous Animals and Toxins including Tropical Diseases, 16 (4): 599-606.
  7. Ozkan, O., Adigüzel, S., Yaki Tiran, S., Cesaretli, Y., Orman, M., Karaer, K. Z. (2006). Androctonus crassicauda (Olivier 1807) scorpionism in the Sanliurfa provinces of Turkey. Turkiye Parazitol. Derg,. 30: 239–245.
  8. Ozkan O. and Karaer, Z. (2003). The scorpions in Turkey. Turk. Bull. Hyg. Exp. Biol., 60: 55-62. 
  9. Rozas, J., Sanchez-DelBarrio, J.C., Messeguer , X. and Rozas, R. (2003). DnaSP DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19: 2496–2497.
  10. Saikia, J., Roy, T.C., Naskar, S., Das, B. and Ferdoci, A.M. (2015). Molecular characterization of cytochrome B gene in indigenous pig. Indian J. Anim. Res., 49 (2): 196-198
  11. Sobita, N. and Basudha, C. (2017). Molecular phylogeny of Barbin fishes of North - East “India based on mitochondrial 16SrRNA gene sequences. Indian J. Anim. Res., 51 (6): 1062-1065.
  12. Yagmur, E. A. (2005). Scorpions of Gaziantep (Ordo:Scorpiones) and their zoogeographic distributions. MSc. thesis, Gaziantep University, Gaziantep, 150p.
  13. Yagmur, E. A., Koç, H., Kesmezoglu, S. and Yalçin, M. (2007). Scorpions of Kilis Province, Turkey (Arachnida: Scorpiones). Serket, 10(3): 91-105.
  14. Yagmur, E. A. (2011). Southeastern Anatolia Scorpion (Arachnida: Scorpiones) Faunasi: Systematics and Zoogeography. Phd Thesis, Ege Üniversity, Izmir, 202pp.
  15. Yazgan, K. (2017). Determining heat stress effect in Holstein dairy cattle using daily milk yield and meteorological data obtained from public weather station in Sanliurfa province of Turkey. Indian J. Anim. Res., 51 (6): 1002-1011.
  16. Yesilyurt, F. (2005). Systematics and Bioecology of Some Acreplains in Anatolia (Arachnida, Scorpionida). MSc. thesis, Kirikkale University, Kirikkale, 91pp.
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    Research Article

    volume 53 issue 2 (february 2019) : 187-191,   Doi: 10.18805/ijar.B-862

    Mitochondrial cytochrome oxidase subunit I-sequence variation in three scorpion species from southeast Turkey 

    S
    Sahin Toprak
    A
    Arif Parmaksiz
    N
    Nevin Aslan
    1Harran University, Faculty of Science and Art, Department of Biology, Sanliurfa - 63100, Turkey.
    Cite article:- Toprak Sahin, Parmaksiz Arif, Aslan Nevin (2018). Mitochondrial cytochrome oxidase subunit I-sequence variation in three scorpion species from southeast Turkey. Indian Journal of Animal Research. 53(2): 187-191. doi: 10.18805/ijar.B-862.

    ABSTRACT

    Southeastern Anatolia is the richest region in Turkey in terms of scorpion fauna. In this region, a number of studies on the fauna of scorpions, as well as epidemiology and clinical findings of scorpion stings in terms of public health, have been carried out, but there is no study on the phylogenetic relationships between species. In this study, 18 individuals of three species were collected from five locations. Sequence analysis was conducted by amplification of a 546 base region from part of mtDNA COI region. Six polymorphic regions and four haplotypes were determined for A. crassicauda, a species important for humans and mean haplotype and nucleotide variations were 0.602 and 0.00555 respectively. Sequences from all individuals were subjected to the blast tool of the NCBI database and assessed with close species, so a phylogenetic tree was formed. On the tree, samples from Þanlýurfa and Diyarbakýr were on different branches while the sample from Iran (HM567333) was on a branch from the other two. The species that was the closest to Scorpio maurus from Batman (KF953944) was the sample from Diyarbakýr. Because there was no other sequence for Buthacus macrocentrus, it was located close to the Buthacus sp. (KF548116) sample. As a result, the locations of sample species were determined as a result of phylogenetic analyses. 

    REFERENCES

    1. Crucitti, P., and V. Vignoli (2002). Gli Scorpioni (Scorpiones) dell’Anatolia sud-orientale (Turchia). Boll. Mus. Sci. Nat. Torino, 19(2), 433-474.
    2. Folmer, O., Black, M., Hoeh, W., Lutz, R. and Vrijenhoek, R. (1994). DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology, 3: 294–299. 
    3. Karatas, A., and Çolak, M. (2005). Scorpions of Gaziantep Province, Turkey (Arachnida: Scorpiones). Euscorpius, 30: 1-7.
    4. Kumar, S., Stecher, G. and Tamura, K. (2016). Molecular evolutionary genetics analysis version 7.0 (MEGA 7) for bigger datasets. Molecular Biology and Evolution. 28: 2731-2739. 
    5. Kürtüllü, M. (2006). An investigation of the scorpion species (ordo: Scorpiones) of Mardin province. MSc. Thesis. Nigde University, Nigde, 51p.
    6. Ozkan, O., Ahmet, C. and Zafer, K. (2010). A study on the genetic diversity of Androctonus crassicauda (Olivier, 1807; Scorpiones: Buthidae) from Turkey. Journal of Venomous Animals and Toxins including Tropical Diseases, 16 (4): 599-606.
    7. Ozkan, O., Adigüzel, S., Yaki Tiran, S., Cesaretli, Y., Orman, M., Karaer, K. Z. (2006). Androctonus crassicauda (Olivier 1807) scorpionism in the Sanliurfa provinces of Turkey. Turkiye Parazitol. Derg,. 30: 239–245.
    8. Ozkan O. and Karaer, Z. (2003). The scorpions in Turkey. Turk. Bull. Hyg. Exp. Biol., 60: 55-62. 
    9. Rozas, J., Sanchez-DelBarrio, J.C., Messeguer , X. and Rozas, R. (2003). DnaSP DNA polymorphism analyses by the coalescent and other methods. Bioinformatics, 19: 2496–2497.
    10. Saikia, J., Roy, T.C., Naskar, S., Das, B. and Ferdoci, A.M. (2015). Molecular characterization of cytochrome B gene in indigenous pig. Indian J. Anim. Res., 49 (2): 196-198
    11. Sobita, N. and Basudha, C. (2017). Molecular phylogeny of Barbin fishes of North - East “India based on mitochondrial 16SrRNA gene sequences. Indian J. Anim. Res., 51 (6): 1062-1065.
    12. Yagmur, E. A. (2005). Scorpions of Gaziantep (Ordo:Scorpiones) and their zoogeographic distributions. MSc. thesis, Gaziantep University, Gaziantep, 150p.
    13. Yagmur, E. A., Koç, H., Kesmezoglu, S. and Yalçin, M. (2007). Scorpions of Kilis Province, Turkey (Arachnida: Scorpiones). Serket, 10(3): 91-105.
    14. Yagmur, E. A. (2011). Southeastern Anatolia Scorpion (Arachnida: Scorpiones) Faunasi: Systematics and Zoogeography. Phd Thesis, Ege Üniversity, Izmir, 202pp.
    15. Yazgan, K. (2017). Determining heat stress effect in Holstein dairy cattle using daily milk yield and meteorological data obtained from public weather station in Sanliurfa province of Turkey. Indian J. Anim. Res., 51 (6): 1002-1011.
    16. Yesilyurt, F. (2005). Systematics and Bioecology of Some Acreplains in Anatolia (Arachnida, Scorpionida). MSc. thesis, Kirikkale University, Kirikkale, 91pp.
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