Indian Journal of Animal Research

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Research Article

Indian Journal of Animal Research, volume 52 issue 7 (july 2018) : 1043-1047

In vitro effects of 2, 4-Dichlorophenoxy acetic acid dimethylamine salt and enrofloxacin on BTH 

Mustafa Oguzhan Kaya
1Siirt University, Faculty of Veterinary Medicine, Div. Basic Sciences, Dep. of Biochemistry, Siirt, Turkey
Cite article:- Kaya Oguzhan Mustafa (2017). In vitro effects of 2, 4-Dichlorophenoxy acetic acid dimethylamine salt and enrofloxacin on BTH. Indian Journal of Animal Research. 52(7): 1043-1047. doi: 10.18805/ijar.B-845.

ABSTRACT

In this study, the in vitro effects of enrofloxacin, a broad spectrum antibiotic, and 2, 4-Dichlorophenoxy acetic acid dimethylamine salt that, a herbicide, on the bovine testicular hyaluronidase (BTH) were analyzed. BTH was purified using the method of ammonium sulfate precipitation and using affinity gel (Sepharose-4B-L-tyrosine-m-anisidine). Then the inhibiting effects of enrofloxacin and 2, 4-Dichlorophenoxy acetic acid dimethylamine salt on the purified BTH enzyme were identified. IC50 values of these chemical substances were found to be 0.3101 and 0.4276 mM, respectively.

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