In vitro effects of 2, 4-Dichlorophenoxy acetic acid dimethylamine salt and enrofloxacin on BTH 

DOI: 10.18805/ijar.B-845    | Article Id: B-845 | Page : 1043-1047
Citation :- In vitro effects of 2, 4-Dichlorophenoxy acetic acid dimethylamine salt and enrofloxacin on BTH.Indian Journal Of Animal Research.2018.(52):1043-1047
Mustafa Oguzhan Kaya m.oguzhankaya@gmail.com
Address : Siirt University, Faculty of Veterinary Medicine, Div. Basic Sciences, Dep. of Biochemistry, Siirt, Turkey
Submitted Date : 22-10-2017
Accepted Date : 20-12-2017

Abstract

In this study, the in vitro effects of enrofloxacin, a broad spectrum antibiotic, and 2, 4-Dichlorophenoxy acetic acid dimethylamine salt that, a herbicide, on the bovine testicular hyaluronidase (BTH) were analyzed. BTH was purified using the method of ammonium sulfate precipitation and using affinity gel (Sepharose-4B-L-tyrosine-m-anisidine). Then the inhibiting effects of enrofloxacin and 2, 4-Dichlorophenoxy acetic acid dimethylamine salt on the purified BTH enzyme were identified. IC50 values of these chemical substances were found to be 0.3101 and 0.4276 mM, respectively.

Keywords

Antibiotic Herbicide Hyaluronidase IC50 Inhibition

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