Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.5 (2023)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Indian Journal of Animal Research, volume 52 issue 10 (october 2018) : 1422-1427

Effects of Salvia Miltiorrhiza extract on the regulation of antioxidant enzyme activities in liver and kidney of rats exposed to TCA

Hanan S. Alnahdi, Kholoud S. Ramadan, Hoda E. A. Farid, Najla O. Ayaz
1Department of Biochemistry, Faculty of Science-Al Faisaliah, King Abdulaziz University, Jeddah, Saudi Arabia.
Cite article:- Alnahdi S. Hanan, Ramadan S. Kholoud, Farid A. E. Hoda, Ayaz O. Najla (2018). Effects of Salvia Miltiorrhiza extract on the regulation of antioxidant enzyme activities in liver and kidney of rats exposed to TCA. Indian Journal of Animal Research. 52(10): 1422-1427. doi: 10.18805/ijar.B-793.
The present study aimed to investigate the protective role of Salvia Miltiorrhiza against Trichloroacetic acid (TCA) induced liver and kidney toxicity in rats. Twenty eight adults Wister albino male rats were divided into 4 equal groups. Group 1 served as control, while group 2 received SM extracts at 200 mg/kg body weight; group 3 was treated with 50 mg/kg/day TCA by gavage daily. Group 4 received the extract at 200 mg/kg body weight and 50 mg/kg/day TCA for two months. A significant increase was observed in liver function, kidney function and MDA (malondialdehyde) levels among the TCA administered animals compared to normal control. Daily oral administration of SM normalized most biochemical changes observed among the TCA treated animals. Histopathologically, higher amount of mononuclear cells infiltration, necrotic cells and few fibroblasts were observed in liver and kidney of TCA treated group. The administration of Salvia Miltiorrhiza extract regulates and decreases liver and kidney damage.
  1. Akpinar, D., Yargicoglu, P., Derin, N., Alicigüzel, Y., and Agar, A. (2008). The effect of lipoic acid on antioxidant status and lipid peroxidation in rats exposed to chronic restraint stress. Physiological research, 57(6): 893.
  2. Basu, S. (2003). Carbon tetrachloride-induced lipid peroxidation: eicosanoid formation and their regulation by antioxidant nutrients. Toxicology, 189(1):113-127.
  3. Chen, G., Fu, Y., and Wu, X. (2012). Protective effect of Salvia miltiorrhiza extract against renal ischemia-reperfusion-induced injury in rats. Molecules, 17(2): 1191-1202. Doi:
  4. Halake, K., Birajdar, M., and Lee, J. (2016). Structural implications of polyphenolic antioxidants. Journal of Industrial and Engineering Chemistry, 35: 1-7. 
  5. Hassoun, E. A., Cearfoss, J., and Spildener, J. (2010). Dichloroacetate and trichloroacetate induced oxidative stress in the hepatic tissues of mice after long term exposure. Journal of Applied Toxicology, 30(5): 450-456.
  6. Komulainen, H. (2004). Experimental cancer studies of chlorinated by-products. Toxicology, 198(1): 239-248.    j.tox.2004.01.031
  7. Liu, G. T., Zhang, T. M., Wang, B. E., and Wang, Y. W. (1992). Protective action of seven natural phenolic compounds against peroxidative damage to biomembranes. Biochemical Pharmacology, 43(2): 147-152. Doi:    2952(92)90271-j
  8. Murugesan, P., Muthusamy, T., Balasubramanian, K., and Arunakaran, J. (2007). Effects of vitamins C and E on steroidogenic enzymes mRNA expression in polychlorinated biphenyl (Aroclor 1254) exposed adult rat Leydig cells. Toxicology, 232(3): 170-182.
  9. Pramyothin, P., Janthasoot, W., Pongnimitprasert, N., Phrukudom, S., and Ruangrungsi, N. (2004). Hepatotoxic effect of (+) usnic acid from Usnea siamensis Wainio in rats, isolated rat hepatocytes and isolated rat liver mitochondria. Journal of Ethnopharmacology, 90(2): 381-387.
  10. Salah, E. B. S. B., El Arem, A., Louedi, M., Saoudi, M., Elfeki, A., Zakhama, A., and Achour, L. (2012). Antioxidant-rich date palm fruit extract inhibits oxidative stress and nephrotoxicity induced by dimethoate in rat. Journal of Physiology and Biochemistry, 68(1): 47-58.
  11. Scaduto, R. C., Gattone, V. H., Grotyohann, L. W., Wertz, J., and Martin, L. F. (1988). Effect of an altered glutathione content on renal ischemic injury. American Journal of Physiology-Renal Physiology, 255(5): F911-F921. Doi:    000173414
  12. Shi, M., Luo, X., Ju, G., Yu, X., Hao, X., Huang, Q., and Kai, G. (2014). Increased accumulation of the cardio-cerebrovascular disease treatment drug tanshinone in Salvia miltiorrhiza hairy roots by the enzymes 3-hydroxy-3-methylglutaryl CoA reductase and 1-deoxy-D-xylulose 5-phosphate reductoisomerase. Functional & Integrative Genomics, 14(3):    s10142-014-0385-0
  13. Sun, J., Huang, S. H., Tan, B. K. H., Whiteman, M., Zhu, Y. C., Wu, Y. J., and Zhu, Y. Z. (2005). Effects of purified herbal extract of Salvia miltiorrhiza on ischemic rat myocardium after acute myocardial infarction. Life Sciences, 76(24): 2849-2860.
  14. Tunalier, Z., Kosar, M., Ozturk, N., Baser, K. H. C., Duman, H., and Kirimer, N. (2004). Antioxidant properties and phenolic composition of Sideritis species. Chemistry of Natural Compounds, 40(3): 206-210.
  15. Yang, J., Li, Y., Wang, F., and Wu, C. (2010). Hepatoprotective effects of apple polyphenols on CCl4-induced acute liver damage in mice. Journal of Agricultural and Food Chemistry, 58(10): 6525-6531..

Editorial Board

View all (0)