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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 8 (august 2018) : 1146-1150,   Doi: 10.18805/ijar.B-791

Effects of negative pressure on boar semen quality during liquid storage at 17°C

J
Jingchun Li
Q
Qi Li
G
Guosheng Wei
J
Jiabao Zhang
Y
Yanbing Li
1College of Animal Sciences, Jilin University, Changchun, P. R. China
Cite article:- Li Jingchun, Li Qi, Wei Guosheng, Zhang Jiabao, Li Yanbing (2017). Effects of negative pressure on boar semen quality during liquid storage at 17°C. Indian Journal of Animal Research. 52(8): 1146-1150. doi: 10.18805/ijar.B-791.

ABSTRACT

This study aimed to investigate the influence of negative pressure on boar semen quality during liquid preservation at 17°C. Semen samples from ten large white boars were collected and pooled, divided into four equal parts, and diluted with Modena containing 0.4% (w/v) of bovine serum albumin. The semen samples were placed in a closed container with valve, and a negative pressure was applied for 2–5 minutes using a vacuum pump with a barometer. The control group had no treatment, the P200 group was treated at 200 mbar, the P400 group at 400 mbar, and the P800 group at 800 mbar. During liquid preservation, sperm motility, total antioxidant capacity, and semen H2O2 content were analyzed every 24 h. The effective survival time of boar semen during preservation was evaluated. The results indicated that a suitable negative pressure decreased the effects on reactive oxygen species on boar sperm quality during liquid preservation compared with the control group. Among all the groups, the 400 mbar negative pressure group had the highest sperm motility, total antioxidant capacity, and the percentage of spermatozoa with high mitochondrial membrane potential. The P400 group also had semen H2O2 content than the other groups. A suitable negative pressure improves sperm quality by reducing oxidative stress and the respiratory metabolism of sperm, and the optimum negative pressure is 400 mbar.

REFERENCES

  1. Alvarez, J.G., and Storey, B.T. (1995). Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol. Reprod. Dev., 42: 334-346.
  2. Bucak, M.N., Sarýözkan, S., Tuncer, P.B., Sakin, F., Ateþþahin, A., Kulaksýz, R., Çevikf, M. (2010). The effect of antioxidants on post-thawed Angora goat (capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Ruminant Res., 89: 24-30.
  3. Casali, R., Silva, L.G., Arcego, C.C., Mozzaquatro, F.D., Mezzalira, A. (2015). Negative pressure in the pre-freezing of ram semen. Acta Scientiae Veterinariae, 42: 1–6.
  4. Cerolini, S., Maldjian, A., Surai, P., Noble, R. (2000). Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim. Reprod. Sci., 58: 99-111.
  5. Fantinati, P., Zannoni, A., Bernardini, C., Forni, M., Tattini, A., Seren, E., Bacci, M.L. (2009). Evaluation of swine fertilisation medium (sfm) efficiency in preserving spermatozoa quality during long-term storage in comparison to four commercial swine extenders. Animal, 3: 269-74.
  6. Frydrychová, S., Lustyková, A., Václavková, E., Lipenský J., Rozkot, M. (2015). Effect of different extenders on quality of frozen-    thawed boar semen. Indian J. Anim. Res., 49: 851-854.
  7. Gerrits, R. J., Lunney, J. K., Johnson, L. A., Pursel, V. G., Kraeling, R. R., Rohrer, G. A., Dobrinskya, J.R. (2005). Perspectives for artificial insemination and genomics to improve global swine populations. Theriogenology, 63: 283-299.
  8. Gil Ananya, M.C., Barón, F.J., Guerrero, J.M., García-Marín, L.J., Gil, J. (2014). Increasing extender viscosity improves the quality of cooled boar semen. J. Agri. Sci., 6: 12-22.
  9. Gogol, P., Szczeœniak-Fabiañczyk, B., Wierzchoœ-Hilczer, A. (2009). The photon emission, ATP level and motility of boar spermatozoa during liquid storage. Reprod. Biol., 9: 39-49.
  10. Hu, J.H., Li, Q.W., Zhang, T., Jiang, Z.L. (2009). Effect of gynostemma pentaphyllum polysaccharide on boar spermatozoa quality following freezing-thawing. Cryobiology, 59: 244-249.
  11. Huo, L.J., Ma, X.H., Yang, Z.M. (2002). Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage. Theriogenology, 58:1349-1360.
  12. Johnson, L.A., Weitze, K.F., Fiser, P., Maxwell, W.M. (2000). Storage of boar semen. Anim. Reprod. Sci., 62(1-3): 143-172.
  13. Lalrintluanga, K., Deka, B.C., Nath, K.C., Sarmah, B.C., Biswas, R.K. (2014). Effect of heterospermy on the quality of spermatozoa during preservation of boar semen at 18°C. Indian J. Anim. Res., 48: 201-203.
  14. Leahy, T., and Gadella, B.M. (2011). Capacitation and capacitation-like sperm surface changes induced by handling boar semen. Reprod. Dom. Anim., 46 Suppl 2(s2): 7-13.
  15. Li, J.C, Zhu, S.B., He, X.J., Sun, R., He, Q.Y., Gan, Y., Liu, S.J., Funahashi, H., Li, Y.B. (2016). Application of a microfluidic sperm sorter to in vitro production of dairy cattle sex-sorted embryos. Theriogenology, 85:1211-1218.
  16. Pandey, R.P., Singh, B.K. (2001): Effect of dilutors on extracellular enzyme activity of preserved boar semen. Indian J. Anim. Sci., 71: 826-828.
  17. Pursel, V.G., Schulman, L.L., Johnson, L.A. (1978). Effect of orvus es paste on acrosome morphology, motility and fertilizing capacity of frozen-thawed boar sperm. J. Anim. Sci., 47: 198-202.
  18. Roca, J., Rodríguez, M.J., Gil, M. A., Carvajal, G., Garcia, E.M., Cuello, C., Vazquez, J.M., Martinez, E.A. (2005). Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. J. Androl., 26: 15-24.
  19. Sarýözkan, S., Türk, G., Cantürk, F., Yay, A., Eken, A., Akçay, A. (2013). The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67: 1-6.
  20. Watson, P.F. (1990). Artificial insemination and the preservation of semen. In: Lamming G (ed.), Marshall’s Physiology of Reproduction. Churchill Livingstone, Edinburgh, London, UK: 747–869.
  21. Yi, Y.J., Li, Z.H., Kim, E.S., Song, E.S., Kim, H.B., Cong, P.Q., Lee, J.M., Park, C.S. (2008). Comparison of motility, acrosome, viability and ATP of boar sperm with or without cold shock resistance in liquid semen at 17°C and 4°C, and frozen-thawed semen. Asian Australasian J. Anim. Sci., 21: 190-197.
  22. Zhang, X.G., Liu, Q., Wang, L.Q., Yang, G. S., Hu, J.H. (2016). Effects of glutathione on sperm quality during liquid storage in boars. Anim. Sci. J., 87: 1195-1201.
  23. Zhang, X.G., Yan, G.J., Hong, J. Y., Su, Z.Z., Yang, G.S., Li, Q.W., Hu, J.H. (2015). Effects of bovine serum albumin on boar sperm quality during liquid storage at 17°C. Reprod. Dom. Anim., 50: 263-269. 
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 8 (august 2018) : 1146-1150,   Doi: 10.18805/ijar.B-791

    Effects of negative pressure on boar semen quality during liquid storage at 17°C

    J
    Jingchun Li
    Q
    Qi Li
    G
    Guosheng Wei
    J
    Jiabao Zhang
    Y
    Yanbing Li
    1College of Animal Sciences, Jilin University, Changchun, P. R. China
    Cite article:- Li Jingchun, Li Qi, Wei Guosheng, Zhang Jiabao, Li Yanbing (2017). Effects of negative pressure on boar semen quality during liquid storage at 17°C. Indian Journal of Animal Research. 52(8): 1146-1150. doi: 10.18805/ijar.B-791.

    ABSTRACT

    This study aimed to investigate the influence of negative pressure on boar semen quality during liquid preservation at 17°C. Semen samples from ten large white boars were collected and pooled, divided into four equal parts, and diluted with Modena containing 0.4% (w/v) of bovine serum albumin. The semen samples were placed in a closed container with valve, and a negative pressure was applied for 2–5 minutes using a vacuum pump with a barometer. The control group had no treatment, the P200 group was treated at 200 mbar, the P400 group at 400 mbar, and the P800 group at 800 mbar. During liquid preservation, sperm motility, total antioxidant capacity, and semen H2O2 content were analyzed every 24 h. The effective survival time of boar semen during preservation was evaluated. The results indicated that a suitable negative pressure decreased the effects on reactive oxygen species on boar sperm quality during liquid preservation compared with the control group. Among all the groups, the 400 mbar negative pressure group had the highest sperm motility, total antioxidant capacity, and the percentage of spermatozoa with high mitochondrial membrane potential. The P400 group also had semen H2O2 content than the other groups. A suitable negative pressure improves sperm quality by reducing oxidative stress and the respiratory metabolism of sperm, and the optimum negative pressure is 400 mbar.

    REFERENCES

    1. Alvarez, J.G., and Storey, B.T. (1995). Differential incorporation of fatty acids into and peroxidative loss of fatty acids from phospholipids of human spermatozoa. Mol. Reprod. Dev., 42: 334-346.
    2. Bucak, M.N., Sarýözkan, S., Tuncer, P.B., Sakin, F., Ateþþahin, A., Kulaksýz, R., Çevikf, M. (2010). The effect of antioxidants on post-thawed Angora goat (capra hircus ancryrensis) sperm parameters, lipid peroxidation and antioxidant activities. Small Ruminant Res., 89: 24-30.
    3. Casali, R., Silva, L.G., Arcego, C.C., Mozzaquatro, F.D., Mezzalira, A. (2015). Negative pressure in the pre-freezing of ram semen. Acta Scientiae Veterinariae, 42: 1–6.
    4. Cerolini, S., Maldjian, A., Surai, P., Noble, R. (2000). Viability, susceptibility to peroxidation and fatty acid composition of boar semen during liquid storage. Anim. Reprod. Sci., 58: 99-111.
    5. Fantinati, P., Zannoni, A., Bernardini, C., Forni, M., Tattini, A., Seren, E., Bacci, M.L. (2009). Evaluation of swine fertilisation medium (sfm) efficiency in preserving spermatozoa quality during long-term storage in comparison to four commercial swine extenders. Animal, 3: 269-74.
    6. Frydrychová, S., Lustyková, A., Václavková, E., Lipenský J., Rozkot, M. (2015). Effect of different extenders on quality of frozen-    thawed boar semen. Indian J. Anim. Res., 49: 851-854.
    7. Gerrits, R. J., Lunney, J. K., Johnson, L. A., Pursel, V. G., Kraeling, R. R., Rohrer, G. A., Dobrinskya, J.R. (2005). Perspectives for artificial insemination and genomics to improve global swine populations. Theriogenology, 63: 283-299.
    8. Gil Ananya, M.C., Barón, F.J., Guerrero, J.M., García-Marín, L.J., Gil, J. (2014). Increasing extender viscosity improves the quality of cooled boar semen. J. Agri. Sci., 6: 12-22.
    9. Gogol, P., Szczeœniak-Fabiañczyk, B., Wierzchoœ-Hilczer, A. (2009). The photon emission, ATP level and motility of boar spermatozoa during liquid storage. Reprod. Biol., 9: 39-49.
    10. Hu, J.H., Li, Q.W., Zhang, T., Jiang, Z.L. (2009). Effect of gynostemma pentaphyllum polysaccharide on boar spermatozoa quality following freezing-thawing. Cryobiology, 59: 244-249.
    11. Huo, L.J., Ma, X.H., Yang, Z.M. (2002). Assessment of sperm viability, mitochondrial activity, capacitation and acrosome intactness in extended boar semen during long-term storage. Theriogenology, 58:1349-1360.
    12. Johnson, L.A., Weitze, K.F., Fiser, P., Maxwell, W.M. (2000). Storage of boar semen. Anim. Reprod. Sci., 62(1-3): 143-172.
    13. Lalrintluanga, K., Deka, B.C., Nath, K.C., Sarmah, B.C., Biswas, R.K. (2014). Effect of heterospermy on the quality of spermatozoa during preservation of boar semen at 18°C. Indian J. Anim. Res., 48: 201-203.
    14. Leahy, T., and Gadella, B.M. (2011). Capacitation and capacitation-like sperm surface changes induced by handling boar semen. Reprod. Dom. Anim., 46 Suppl 2(s2): 7-13.
    15. Li, J.C, Zhu, S.B., He, X.J., Sun, R., He, Q.Y., Gan, Y., Liu, S.J., Funahashi, H., Li, Y.B. (2016). Application of a microfluidic sperm sorter to in vitro production of dairy cattle sex-sorted embryos. Theriogenology, 85:1211-1218.
    16. Pandey, R.P., Singh, B.K. (2001): Effect of dilutors on extracellular enzyme activity of preserved boar semen. Indian J. Anim. Sci., 71: 826-828.
    17. Pursel, V.G., Schulman, L.L., Johnson, L.A. (1978). Effect of orvus es paste on acrosome morphology, motility and fertilizing capacity of frozen-thawed boar sperm. J. Anim. Sci., 47: 198-202.
    18. Roca, J., Rodríguez, M.J., Gil, M. A., Carvajal, G., Garcia, E.M., Cuello, C., Vazquez, J.M., Martinez, E.A. (2005). Survival and in vitro fertility of boar spermatozoa frozen in the presence of superoxide dismutase and/or catalase. J. Androl., 26: 15-24.
    19. Sarýözkan, S., Türk, G., Cantürk, F., Yay, A., Eken, A., Akçay, A. (2013). The effect of bovine serum albumin and fetal calf serum on sperm quality, DNA fragmentation and lipid peroxidation of the liquid stored rabbit semen. Cryobiology, 67: 1-6.
    20. Watson, P.F. (1990). Artificial insemination and the preservation of semen. In: Lamming G (ed.), Marshall’s Physiology of Reproduction. Churchill Livingstone, Edinburgh, London, UK: 747–869.
    21. Yi, Y.J., Li, Z.H., Kim, E.S., Song, E.S., Kim, H.B., Cong, P.Q., Lee, J.M., Park, C.S. (2008). Comparison of motility, acrosome, viability and ATP of boar sperm with or without cold shock resistance in liquid semen at 17°C and 4°C, and frozen-thawed semen. Asian Australasian J. Anim. Sci., 21: 190-197.
    22. Zhang, X.G., Liu, Q., Wang, L.Q., Yang, G. S., Hu, J.H. (2016). Effects of glutathione on sperm quality during liquid storage in boars. Anim. Sci. J., 87: 1195-1201.
    23. Zhang, X.G., Yan, G.J., Hong, J. Y., Su, Z.Z., Yang, G.S., Li, Q.W., Hu, J.H. (2015). Effects of bovine serum albumin on boar sperm quality during liquid storage at 17°C. Reprod. Dom. Anim., 50: 263-269. 
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