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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 1 (january 2019) : 1-7,   Doi: 10.18805/ijar.B-783

An idiogram on pachytene bivajents with high resalution multiple bands of Epinephelus malabaricus

A
Aiguo Zhou
S
Shaolin Xie
Z
Zhenlu Wang
Y
Yanfeng Chen
Y
Yongyong Feng
C
Chao Wang
Q
Qiao Ye
L
Lanfen Fan
M
Meifang Wang
J
Jixing Zou
1College of Marine Sciences, South China Agricultural University, Guangzhou-510 642, Guangdong, China
Cite article:- Zhou Aiguo, Xie Shaolin, Wang Zhenlu, Chen Yanfeng, Feng Yongyong, Wang Chao, Ye Qiao, Fan Lanfen, Wang Meifang, Zou Jixing (2017). An idiogram on pachytene bivajents with high resalution multiple bands of Epinephelus malabaricus. Indian Journal of Animal Research. 53(1): 1-7. doi: 10.18805/ijar.B-783.

ABSTRACT

Epinephelus malabaricus (Perciformes, Serranidae), a rare marine commercial fish inhabits in the warm-water reef along the southeast coast of China. In the present study, well-spread pachytene bivalents with multiple high-resolution bands were obtained after the testes were treated with a hypotonic alkaline solution and a high-chloroform fixative solution, showing that the number and characteristics of band in each bivalent were stable. According to karyotype analysis, the haploid chromosome set was n=24 (24t), and no heteromorphic bivalent was found. The relative length of the longest and shortest bivalent were 5.86 and 1.66, and the length ratio was 3.53, which was much higher than the length ratio of the exact metaphase mitotic karyotype (1.5) and premetaphase endonuclease karyotype (1.75). An idiogram with 447 bands was constructed, and the zone of each bivalent was divided, respectively. The detailed description of the landmark system and the band positions were preliminary given. This study aims to provide a theoretical reference for the further research on cytogenetics and the physical map construction of chromosome fine frame of E. malabaricus.

REFERENCES

  1. Bhat PP and Rawat AK (1990). Banded karyotypes of Indian goat breeds. Indian Journal of Animal Sciences, 60(7): 880-883.
  2. Chang ZJ, Du QY and Yu QX (2001). Pachytene Chromosome Banding of Paramisgurnus dabryanus. Chinese Journal of zoology, 36(1):23-26. 
  3. Dam Roy S and George G (2010). Marine resources of islands: status and approaches for sustainable exploitation/ conservation with special emphasis to Andaman and Nicobar. Indian Journal of Animal Sciences, 80(4): 57-62.
  4. Gold JR, Li YC, Shipley NS and Powers PK (1990). Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. J. Fish Biology, 37:563-575. 
  5. Guo M., Wang S, Su Y, Zhou Y, Liu M and Wang J (2014). Molecular cytogenetic analyses of Epinephelus bruneus and Epinephelus moara (Perciformes, Epinephelidae). PeerJ, 2: e412.
  6. Hong MX and Yang JH (1988). Studies on the Karyotype of Epinephelus awoara. Journal of Xiamen University (Natural Science), 2:62-68. 
  7. Hong YH and Zhou T (1985). Chromosome Banding in Fishes ‘!. An Improved BrdU-Hoechst-Giemsa Method for Revealing DNA-    Replication Bands in Fish Chromosomes. Acta Genetica Sinica, 12(1):67-71.
  8. Jagillo GM and Fanf JS (1982). Complete autosomal chromomere maps of human early and mid/late pachytene spermatocytes. Am. J. Hum. Genet, 34:112-124. 
  9. Khan TA, Bhise MP and Lakra WS (2000). Chromosome manipulation in fish-a review. Indian Journal of Animal Sciences, 70(2): 213-221.
  10. MacDonald JR, Ziman R, Yuen RKC, Feuk L and Scherer SW (2014). The database of genomic variants: a curated collection of structural variation in the human genome. Nucleic Acids Research, 42(1): 986-992.
  11. Maneechot N and Supiwong W (2015). Cytogenetics of Greasy Grouper, Epinephelus tauvina (Perciformes, Serranidae) by Conventional Staining and Ag-NOR Banding Techniques. Journal of Fisheries Technology Research, 9(1-6):53-63.
  12. Natarajan R and Subrahmanyam K (1974). A karyotype study of some teleost from Portonovo waters. Proc. Ind, Acad. Sci., 79(5):173-196.
  13. Noikotr K, Pinthong K, Tanomtong A, Sudmoon R, Chaveerach A and Tanee T (2014). Karyotype analysis of two groupers, Epinephelus species (Serranidae). Caryologia, 1:63-65.
  14. Pinthong K, Gomontean B, Kongim B, Khakhong S, Sriveerachai T and Supiwong W (2013). Cytogenetic comparison and chromosome localization of the nucleolar organizer region of four grouper genera (Pisces, Epinephelinae) from Thailand. Cytologia, 78(3):223-234. 
  15. Schmid M and Steinlein C (2015), Chromosome Banding in Amphibia. XXXII. The Genus Xenopus (Anura, Pipidae). Cytogenetic and genome research, 145(3-4):201-217.
  16. Wang SF, Cai Y, Qin YX, Zhou YC, Su YQ and Wang J (2012). Characterization of yellow grouper Epinephelus awoara (Serranidae) karyotype by chromosome bandings and fluorescence in situ hybridization. Journal of Fish Biology, 40:866–875.
  17. Yi MS, Yu QX and Huang L (2002). Construction of high-resolution multi-band ideograph of zebra fish pachytene-stage bivalents. China Science (collection C), 32(3):240-247. 
  18. Yu QX, Pan LC and Cui JX (1993), High-resolution G Band preparation and Primary construction on bivalents of Monopterus albus. Science in China (Series B), 23(9):947-954.
  19. Yu ZN, Kong XY and Xie ZY (1994). Preliminary study of high-resolution banding of Lateolabrax japonicus chromosomes. Essay collections in memorization of the 60th anniversary of the founding of China Zoological Society, Beijing, China Science and Technology Press, pp. 469-473.
  20. Zhou AG, Chen JT, Xie SL, Chen YF, Wang C and ZOU JX (2015). Banding techniques and painting on chromosome of Epinephelus malabaricus. Acta Hydrobiologica Sinica, 39(1):110-116. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 1 (january 2019) : 1-7,   Doi: 10.18805/ijar.B-783

    An idiogram on pachytene bivajents with high resalution multiple bands of Epinephelus malabaricus

    A
    Aiguo Zhou
    S
    Shaolin Xie
    Z
    Zhenlu Wang
    Y
    Yanfeng Chen
    Y
    Yongyong Feng
    C
    Chao Wang
    Q
    Qiao Ye
    L
    Lanfen Fan
    M
    Meifang Wang
    J
    Jixing Zou
    1College of Marine Sciences, South China Agricultural University, Guangzhou-510 642, Guangdong, China
    Cite article:- Zhou Aiguo, Xie Shaolin, Wang Zhenlu, Chen Yanfeng, Feng Yongyong, Wang Chao, Ye Qiao, Fan Lanfen, Wang Meifang, Zou Jixing (2017). An idiogram on pachytene bivajents with high resalution multiple bands of Epinephelus malabaricus. Indian Journal of Animal Research. 53(1): 1-7. doi: 10.18805/ijar.B-783.

    ABSTRACT

    Epinephelus malabaricus (Perciformes, Serranidae), a rare marine commercial fish inhabits in the warm-water reef along the southeast coast of China. In the present study, well-spread pachytene bivalents with multiple high-resolution bands were obtained after the testes were treated with a hypotonic alkaline solution and a high-chloroform fixative solution, showing that the number and characteristics of band in each bivalent were stable. According to karyotype analysis, the haploid chromosome set was n=24 (24t), and no heteromorphic bivalent was found. The relative length of the longest and shortest bivalent were 5.86 and 1.66, and the length ratio was 3.53, which was much higher than the length ratio of the exact metaphase mitotic karyotype (1.5) and premetaphase endonuclease karyotype (1.75). An idiogram with 447 bands was constructed, and the zone of each bivalent was divided, respectively. The detailed description of the landmark system and the band positions were preliminary given. This study aims to provide a theoretical reference for the further research on cytogenetics and the physical map construction of chromosome fine frame of E. malabaricus.

    REFERENCES

    1. Bhat PP and Rawat AK (1990). Banded karyotypes of Indian goat breeds. Indian Journal of Animal Sciences, 60(7): 880-883.
    2. Chang ZJ, Du QY and Yu QX (2001). Pachytene Chromosome Banding of Paramisgurnus dabryanus. Chinese Journal of zoology, 36(1):23-26. 
    3. Dam Roy S and George G (2010). Marine resources of islands: status and approaches for sustainable exploitation/ conservation with special emphasis to Andaman and Nicobar. Indian Journal of Animal Sciences, 80(4): 57-62.
    4. Gold JR, Li YC, Shipley NS and Powers PK (1990). Improved methods for working with fish chromosomes with a review of metaphase chromosome banding. J. Fish Biology, 37:563-575. 
    5. Guo M., Wang S, Su Y, Zhou Y, Liu M and Wang J (2014). Molecular cytogenetic analyses of Epinephelus bruneus and Epinephelus moara (Perciformes, Epinephelidae). PeerJ, 2: e412.
    6. Hong MX and Yang JH (1988). Studies on the Karyotype of Epinephelus awoara. Journal of Xiamen University (Natural Science), 2:62-68. 
    7. Hong YH and Zhou T (1985). Chromosome Banding in Fishes ‘!. An Improved BrdU-Hoechst-Giemsa Method for Revealing DNA-    Replication Bands in Fish Chromosomes. Acta Genetica Sinica, 12(1):67-71.
    8. Jagillo GM and Fanf JS (1982). Complete autosomal chromomere maps of human early and mid/late pachytene spermatocytes. Am. J. Hum. Genet, 34:112-124. 
    9. Khan TA, Bhise MP and Lakra WS (2000). Chromosome manipulation in fish-a review. Indian Journal of Animal Sciences, 70(2): 213-221.
    10. MacDonald JR, Ziman R, Yuen RKC, Feuk L and Scherer SW (2014). The database of genomic variants: a curated collection of structural variation in the human genome. Nucleic Acids Research, 42(1): 986-992.
    11. Maneechot N and Supiwong W (2015). Cytogenetics of Greasy Grouper, Epinephelus tauvina (Perciformes, Serranidae) by Conventional Staining and Ag-NOR Banding Techniques. Journal of Fisheries Technology Research, 9(1-6):53-63.
    12. Natarajan R and Subrahmanyam K (1974). A karyotype study of some teleost from Portonovo waters. Proc. Ind, Acad. Sci., 79(5):173-196.
    13. Noikotr K, Pinthong K, Tanomtong A, Sudmoon R, Chaveerach A and Tanee T (2014). Karyotype analysis of two groupers, Epinephelus species (Serranidae). Caryologia, 1:63-65.
    14. Pinthong K, Gomontean B, Kongim B, Khakhong S, Sriveerachai T and Supiwong W (2013). Cytogenetic comparison and chromosome localization of the nucleolar organizer region of four grouper genera (Pisces, Epinephelinae) from Thailand. Cytologia, 78(3):223-234. 
    15. Schmid M and Steinlein C (2015), Chromosome Banding in Amphibia. XXXII. The Genus Xenopus (Anura, Pipidae). Cytogenetic and genome research, 145(3-4):201-217.
    16. Wang SF, Cai Y, Qin YX, Zhou YC, Su YQ and Wang J (2012). Characterization of yellow grouper Epinephelus awoara (Serranidae) karyotype by chromosome bandings and fluorescence in situ hybridization. Journal of Fish Biology, 40:866–875.
    17. Yi MS, Yu QX and Huang L (2002). Construction of high-resolution multi-band ideograph of zebra fish pachytene-stage bivalents. China Science (collection C), 32(3):240-247. 
    18. Yu QX, Pan LC and Cui JX (1993), High-resolution G Band preparation and Primary construction on bivalents of Monopterus albus. Science in China (Series B), 23(9):947-954.
    19. Yu ZN, Kong XY and Xie ZY (1994). Preliminary study of high-resolution banding of Lateolabrax japonicus chromosomes. Essay collections in memorization of the 60th anniversary of the founding of China Zoological Society, Beijing, China Science and Technology Press, pp. 469-473.
    20. Zhou AG, Chen JT, Xie SL, Chen YF, Wang C and ZOU JX (2015). Banding techniques and painting on chromosome of Epinephelus malabaricus. Acta Hydrobiologica Sinica, 39(1):110-116. 
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