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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 1 (january 2019) : 49-54,   Doi: 10.18805/ijar.B-764

Identification and sequence characterization of LOC427400: a novel gene highly expressed in the Magnum of White leghorn’s Oviduct

Y
Ying Zhang
B
Bolin Ning
J
Jingchun Li
Q
Qianyu He
C
Chunbo Wei
S
Shengjun Liu
1College of Animal Science and Veterinary Medicine, Heilongjiang BAYI Agricultural University, Daqing, Heilongjiang, P. R. China, 163319
Cite article:- Zhang Ying, Ning Bolin, Li Jingchun, He Qianyu, Wei Chunbo, Liu Shengjun (2018). Identification and sequence characterization of LOC427400: a novel gene highly expressed in the Magnum of White leghorn’s Oviduct. Indian Journal of Animal Research. 53(1): 49-54. doi: 10.18805/ijar.B-764.

ABSTRACT

LOC427400 is a novel bird gene located on chromosome Z of the chicken genome. It was deciphered in our previous study by RNA sequencing of different parts of the chicken oviduct. Here we confirmed that LOC427400 expressed significantly high in the magnum comparing with the ovary, isthmus, uterus, liver, jejunum and breast muscle (P<0.05) by quantitative real-time PCR in 5 White Leghorn layers at the age of 34 wk. The full length of transcript sequence of LOC427400 was also obtained by 3’ and 5’ RACE. The new transcript was 2831 bp and the corresponding translated protein was 343 aa. The predicted protein show 2 conserved domains, RICIN and OST-beta, which indicated that LOC427400 protein might have functions like carbohydrate-binding and signal transduction. This study illustrated that LOC47400 should be an important candidate gene related with the process of egg albumen formation and albumen biological functions. 

REFERENCES

  1. Abasht, B., Sandford, E., Arango, J., Settar, P., Fulton, J.E., O’Sullivan, N.P., Hassen, et al. (2009). Extent and consistency of linkage disequilibrium and identification of DNA markers for production and egg quality traits in commercial layer chicken populations. BMC Genomics 10 Suppl 2: S2.
  2. Benton, C.E., Jr., Walsh, T.J. and Brake, J. (2001). Effects of presence of a blastoderm on albumen height and pH of broiler hatching eggs. Poult Sci 80(7): 955-957.
  3. D’Ambrosio, C., Arena, S., Scaloni, A., Guerrier, L., Boschetti, E., Mendieta, M.E., Citterio, A., and Righetti, P.G. (2008). Exploring the chicken egg white proteome with combinatorial peptide ligand libraries. J Proteome Res 7(8): 3461-3474.
  4. Dean, D.M., Jones, P.S. and Sanders M.M. (2001). Alterations in chromatin structure are implicated in the activation of the steroid hormone response unit of the ovalbumin gene. DNA Cell Biol 20(1): 27-39.
  5. Dougherty, D. C. and Sanders M.M. (2005). Estrogen action: revitalization of the chick oviduct model. Trends Endocrinol Metab 16(9): 414-419.
  6. Ellegren, H. (2011). Emergence of male-biased genes on the chicken Z-chromosome: sex-chromosome contrasts between male and female heterogametic systems. Genome Res 21(12): 2082-2086.
  7. Gautron, J., Murayama, E., Vignal, A., Morisson, M., McKee, M.D., Réhault, S., Labas, V., et al. (2007). Cloning of Ovocalyxin-36, a novel chicken eggshell protein related to lipopolysaccharide-binding proteins, bactericidal permeability-increasing proteins, and plunc family proteins. Journal of Biological Chemistry 282(8): 5273-5286.
  8. Guérin-Dubiard, C., Pasco, M., Mollé, D., Désert, C., Croguennec, T. and Nau, F. (2006). Proteomic analysis of hen egg white. J Agric Food Chem 54(11): 3901-3910.
  9. Gupta, C., Kumar, A., Arya, R., Patel, M., Ghosh, A. and Palod, J. (2007). Evaluation of egg quality of crossbred chickens reared under backyard farming in Tarai areas of Uttaranchal. Indian Journal of Animal Research 41(3): 216-219.
  10. Hazes, B. (1996). The (QxW) 3 domain: a flexible lectin scaffold. Protein Science 5(8): 1490-1501.
  11. He, J., Yan, H., and Fan, C. (2014). Optimization of ultrasound-assisted extraction of protein from egg white using response surface methodology (RSM) and its proteomic study by MALDI-TOF-MS. RSC Advances 4(80): 42608-42616.
  12. Hincke, M.T., Gautron, J., Tsang, C.P., McKee, M.D. and Nys, Y. (1999). Molecular cloning and ultrastructural localization of the core protein of an eggshell matrix proteoglycan, ovocleidin-116. J Biol Chem 274(46): 32915-32923.
  13. Hirabayashi, J., Dutta, S.K. and Kasai, K. (1998). Novel galactose-binding proteins in Annelida characterization of 29-kDa tandem repeat-type lectins from the earthworm Lumbricus terrestris. Journal of Biological Chemistry 273(23): 14450-14460.
  14. Honkatukia, M., Tuiskula-Haavisto, M., De Koning, D.J., Virta, A., Mäki-Tanila, A. and Vilkki, J. (2005). A region on chicken chromosome 2 affects both egg white thinning and egg weight. Genet Sel Evol 37(5): 563-577.
  15. Hosseini, S. and Tahmoorespur, M. (2013). Estimation of genetic parameters of various economic traits in west Iranian native chickens. Indian Journal of Animal Research 47(3): 205-211.
  16. Jin, Y.H., Lee, K.T., Lee, W.I. and Han, Y.K. (2011). Effects of storage temperature and time on the quality of eggs from laying hens at peak production. Asian Australas J of Anim Sci 24(2): 279-284.
  17. Kaessmann, H., Vinckenbosch, N. and Long, M. (2009). RNA-based gene duplication: mechanistic and evolutionary insights. Nat Rev Genet 10(1): 19-31.
  18. Long, M., Betran, E., Thornton, K. and Wang, W. (2003). The origin of new genes: glimpses from the young and old. Nat Rev Genet 4(11): 865-875.
  19. Mann, K. (2007). The chicken egg white proteome. Proteomics 7(19): 3558-3568.
  20. Mann, K. and Mann, M. (2011). In-depth analysis of the chicken egg white proteome using an LTQ Orbitrap Velos. Proteome Sci 9(1): 7.
  21. Marchler-Bauer, A., Derbyshire, M.K., Gonzales, N.R., Lu, S., Chitsaz, F., Geer, L.Y., et al (2015). CDD: NCBI’s conserved domain database. Nucleic Acids Res 43: D222-226.
  22. Mine, Y. (2008). Egg Bioscience and Biotechnology. John Wiley and Sons.
  23. Omana, D. A., Liang, Y., Kav, N.N. and Wu, J. (2011). Proteomic analysis of egg white proteins during storage. Proteomics 11(1): 144-153.
  24. Park, H.M., Sanders, M.M., Suzuki, T. and Muramatsu, T. (2006). An oviduct-specific and enhancer-like element resides at about -    3000 in the chicken ovalbumin gene. Biochimie 88(12): 1909-1914.
  25. Raikos, V., Hansen, R., Campbell, L. and Euston, S.R. (2006). Separation and identification of hen egg protein isoforms using SDS–    PAGE and 2D gel electrophoresis with MALDI-TOF mass spectrometry. Food chemistry 99(4): 702-710.
  26. Seal, S., Patel, M.V., Collins, C., Colvin, J. and Bailey, D. (2012). Next generation transcriptome sequencing and quantitative real-    time PCR technologies for characterisation of the Bemisia tabaci Asia 1 mtCOI Phylogenetic Clade. Journal of Integrative Agriculture, 11(2): 281-292.
  27. Silphaduang, U., Colorni, A. and Noga, E.J. (2006). Evidence for widespread distribution of piscidin antimicrobial peptides in teleost fish. Dis Aquat Organ 72(3): 241-252.
  28. Wang, J., Liang, Y., Omana, D.A., Kav, N.N. and Wu, J. (2012). Proteomics analysis of egg white proteins from different egg varieties. J Agric Food Chem 60(1): 272-282.
  29. Yildirim, A., Sekeroglu, A., Koç, H., Eleroglu, H., Duman, M., Tahtali, Y., and Mutlu, M. I. S. (2017). Egg production and quality characteristics of laying hens fed diets supplemented with dry caper (Capparis spinosa) leaf powder. Indian Journal of Animal Research 52:72-78 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 1 (january 2019) : 49-54,   Doi: 10.18805/ijar.B-764

    Identification and sequence characterization of LOC427400: a novel gene highly expressed in the Magnum of White leghorn’s Oviduct

    Y
    Ying Zhang
    B
    Bolin Ning
    J
    Jingchun Li
    Q
    Qianyu He
    C
    Chunbo Wei
    S
    Shengjun Liu
    1College of Animal Science and Veterinary Medicine, Heilongjiang BAYI Agricultural University, Daqing, Heilongjiang, P. R. China, 163319
    Cite article:- Zhang Ying, Ning Bolin, Li Jingchun, He Qianyu, Wei Chunbo, Liu Shengjun (2018). Identification and sequence characterization of LOC427400: a novel gene highly expressed in the Magnum of White leghorn&rsquo;s Oviduct. Indian Journal of Animal Research. 53(1): 49-54. doi: 10.18805/ijar.B-764.

    ABSTRACT

    LOC427400 is a novel bird gene located on chromosome Z of the chicken genome. It was deciphered in our previous study by RNA sequencing of different parts of the chicken oviduct. Here we confirmed that LOC427400 expressed significantly high in the magnum comparing with the ovary, isthmus, uterus, liver, jejunum and breast muscle (P<0.05) by quantitative real-time PCR in 5 White Leghorn layers at the age of 34 wk. The full length of transcript sequence of LOC427400 was also obtained by 3’ and 5’ RACE. The new transcript was 2831 bp and the corresponding translated protein was 343 aa. The predicted protein show 2 conserved domains, RICIN and OST-beta, which indicated that LOC427400 protein might have functions like carbohydrate-binding and signal transduction. This study illustrated that LOC47400 should be an important candidate gene related with the process of egg albumen formation and albumen biological functions. 

    REFERENCES

    1. Abasht, B., Sandford, E., Arango, J., Settar, P., Fulton, J.E., O’Sullivan, N.P., Hassen, et al. (2009). Extent and consistency of linkage disequilibrium and identification of DNA markers for production and egg quality traits in commercial layer chicken populations. BMC Genomics 10 Suppl 2: S2.
    2. Benton, C.E., Jr., Walsh, T.J. and Brake, J. (2001). Effects of presence of a blastoderm on albumen height and pH of broiler hatching eggs. Poult Sci 80(7): 955-957.
    3. D’Ambrosio, C., Arena, S., Scaloni, A., Guerrier, L., Boschetti, E., Mendieta, M.E., Citterio, A., and Righetti, P.G. (2008). Exploring the chicken egg white proteome with combinatorial peptide ligand libraries. J Proteome Res 7(8): 3461-3474.
    4. Dean, D.M., Jones, P.S. and Sanders M.M. (2001). Alterations in chromatin structure are implicated in the activation of the steroid hormone response unit of the ovalbumin gene. DNA Cell Biol 20(1): 27-39.
    5. Dougherty, D. C. and Sanders M.M. (2005). Estrogen action: revitalization of the chick oviduct model. Trends Endocrinol Metab 16(9): 414-419.
    6. Ellegren, H. (2011). Emergence of male-biased genes on the chicken Z-chromosome: sex-chromosome contrasts between male and female heterogametic systems. Genome Res 21(12): 2082-2086.
    7. Gautron, J., Murayama, E., Vignal, A., Morisson, M., McKee, M.D., Réhault, S., Labas, V., et al. (2007). Cloning of Ovocalyxin-36, a novel chicken eggshell protein related to lipopolysaccharide-binding proteins, bactericidal permeability-increasing proteins, and plunc family proteins. Journal of Biological Chemistry 282(8): 5273-5286.
    8. Guérin-Dubiard, C., Pasco, M., Mollé, D., Désert, C., Croguennec, T. and Nau, F. (2006). Proteomic analysis of hen egg white. J Agric Food Chem 54(11): 3901-3910.
    9. Gupta, C., Kumar, A., Arya, R., Patel, M., Ghosh, A. and Palod, J. (2007). Evaluation of egg quality of crossbred chickens reared under backyard farming in Tarai areas of Uttaranchal. Indian Journal of Animal Research 41(3): 216-219.
    10. Hazes, B. (1996). The (QxW) 3 domain: a flexible lectin scaffold. Protein Science 5(8): 1490-1501.
    11. He, J., Yan, H., and Fan, C. (2014). Optimization of ultrasound-assisted extraction of protein from egg white using response surface methodology (RSM) and its proteomic study by MALDI-TOF-MS. RSC Advances 4(80): 42608-42616.
    12. Hincke, M.T., Gautron, J., Tsang, C.P., McKee, M.D. and Nys, Y. (1999). Molecular cloning and ultrastructural localization of the core protein of an eggshell matrix proteoglycan, ovocleidin-116. J Biol Chem 274(46): 32915-32923.
    13. Hirabayashi, J., Dutta, S.K. and Kasai, K. (1998). Novel galactose-binding proteins in Annelida characterization of 29-kDa tandem repeat-type lectins from the earthworm Lumbricus terrestris. Journal of Biological Chemistry 273(23): 14450-14460.
    14. Honkatukia, M., Tuiskula-Haavisto, M., De Koning, D.J., Virta, A., Mäki-Tanila, A. and Vilkki, J. (2005). A region on chicken chromosome 2 affects both egg white thinning and egg weight. Genet Sel Evol 37(5): 563-577.
    15. Hosseini, S. and Tahmoorespur, M. (2013). Estimation of genetic parameters of various economic traits in west Iranian native chickens. Indian Journal of Animal Research 47(3): 205-211.
    16. Jin, Y.H., Lee, K.T., Lee, W.I. and Han, Y.K. (2011). Effects of storage temperature and time on the quality of eggs from laying hens at peak production. Asian Australas J of Anim Sci 24(2): 279-284.
    17. Kaessmann, H., Vinckenbosch, N. and Long, M. (2009). RNA-based gene duplication: mechanistic and evolutionary insights. Nat Rev Genet 10(1): 19-31.
    18. Long, M., Betran, E., Thornton, K. and Wang, W. (2003). The origin of new genes: glimpses from the young and old. Nat Rev Genet 4(11): 865-875.
    19. Mann, K. (2007). The chicken egg white proteome. Proteomics 7(19): 3558-3568.
    20. Mann, K. and Mann, M. (2011). In-depth analysis of the chicken egg white proteome using an LTQ Orbitrap Velos. Proteome Sci 9(1): 7.
    21. Marchler-Bauer, A., Derbyshire, M.K., Gonzales, N.R., Lu, S., Chitsaz, F., Geer, L.Y., et al (2015). CDD: NCBI’s conserved domain database. Nucleic Acids Res 43: D222-226.
    22. Mine, Y. (2008). Egg Bioscience and Biotechnology. John Wiley and Sons.
    23. Omana, D. A., Liang, Y., Kav, N.N. and Wu, J. (2011). Proteomic analysis of egg white proteins during storage. Proteomics 11(1): 144-153.
    24. Park, H.M., Sanders, M.M., Suzuki, T. and Muramatsu, T. (2006). An oviduct-specific and enhancer-like element resides at about -    3000 in the chicken ovalbumin gene. Biochimie 88(12): 1909-1914.
    25. Raikos, V., Hansen, R., Campbell, L. and Euston, S.R. (2006). Separation and identification of hen egg protein isoforms using SDS–    PAGE and 2D gel electrophoresis with MALDI-TOF mass spectrometry. Food chemistry 99(4): 702-710.
    26. Seal, S., Patel, M.V., Collins, C., Colvin, J. and Bailey, D. (2012). Next generation transcriptome sequencing and quantitative real-    time PCR technologies for characterisation of the Bemisia tabaci Asia 1 mtCOI Phylogenetic Clade. Journal of Integrative Agriculture, 11(2): 281-292.
    27. Silphaduang, U., Colorni, A. and Noga, E.J. (2006). Evidence for widespread distribution of piscidin antimicrobial peptides in teleost fish. Dis Aquat Organ 72(3): 241-252.
    28. Wang, J., Liang, Y., Omana, D.A., Kav, N.N. and Wu, J. (2012). Proteomics analysis of egg white proteins from different egg varieties. J Agric Food Chem 60(1): 272-282.
    29. Yildirim, A., Sekeroglu, A., Koç, H., Eleroglu, H., Duman, M., Tahtali, Y., and Mutlu, M. I. S. (2017). Egg production and quality characteristics of laying hens fed diets supplemented with dry caper (Capparis spinosa) leaf powder. Indian Journal of Animal Research 52:72-78 
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