Identification and sequence characterization of LOC427400: a novel gene highly expressed in the Magnum of White leghorn’s Oviduct

DOI: 10.18805/ijar.B-764    | Article Id: B-764 | Page : 49-54
Citation :- Identification and sequence characterization of LOC427400: a novel gene highly expressed in the Magnum of White leghorn’s Oviduct.Indian Journal Of Animal Research.2019.(53):49-54
Ying Zhang, Bolin Ning, Jingchun Li, Qianyu He, Chunbo Wei and Shengjun Liu
Address : College of Animal Science and Veterinary Medicine, Heilongjiang BAYI Agricultural University, Daqing, Heilongjiang, P. R. China, 163319
Submitted Date : 29-04-2017
Accepted Date : 24-02-2018


LOC427400 is a novel bird gene located on chromosome Z of the chicken genome. It was deciphered in our previous study by RNA sequencing of different parts of the chicken oviduct. Here we confirmed that LOC427400 expressed significantly high in the magnum comparing with the ovary, isthmus, uterus, liver, jejunum and breast muscle (P<0.05) by quantitative real-time PCR in 5 White Leghorn layers at the age of 34 wk. The full length of transcript sequence of LOC427400 was also obtained by 3’ and 5’ RACE. The new transcript was 2831 bp and the corresponding translated protein was 343 aa. The predicted protein show 2 conserved domains, RICIN and OST-beta, which indicated that LOC427400 protein might have functions like carbohydrate-binding and signal transduction. This study illustrated that LOC47400 should be an important candidate gene related with the process of egg albumen formation and albumen biological functions. 


Chicken Egg albumen Gene expression LOC427400 gene Rapid amplification of cDNA ends.


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