Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

  • Online ISSN 0976-0555

  • NAAS Rating 6.50

  • SJR 0.263

  • Impact Factor 0.4 (2024)

Frequency :
Monthly (January, February, March, April, May, June, July, August, September, October, November and December)
Indexing Services :
Science Citation Index Expanded, BIOSIS Preview, ISI Citation Index, Biological Abstracts, Scopus, AGRICOLA, Google Scholar, CrossRef, CAB Abstracting Journals, Chemical Abstracts, Indian Science Abstracts, EBSCO Indexing Services, Index Copernicus
Submit

Research Article

Indian Journal of Animal Research, volume 52 issue 3 (march 2018) : 343-346

Phylogenetic analysis based studies on genetic variation of Cytochrome b gene of Indian peafowl (Pavo cristatus) in Pakistan

Junaid Naseer, Khalid Mahmood Anjum, Waseem Ahmad Khan, Muhammad Imran, Muhammad Ishaque, Shahid Hafeez, Muhammad Asif Munir, Muhammad Awais Nazir
1<p>Department of Wildlife and Ecology, University of Veterinary and Animal Sciences, Lahore-Pakistan.</p>
Cite article:- Naseer Junaid, Anjum Mahmood Khalid, Khan Ahmad Waseem, Imran Muhammad, Ishaque Muhammad, Hafeez Shahid, Munir Asif Muhammad, Nazir Awais Muhammad (2017). Phylogenetic analysis based studies on genetic variation of Cytochrome b gene of Indian peafowl (Pavo cristatus) in Pakistan . Indian Journal of Animal Research. 52(3): 343-346. doi: 10.18805/ijar.v0iOF.9119.

ABSTRACT

This study was designed to analyze genetic variation between Indian peafowl available at different locations by targeting the Cytochrome b gene. A total of ten birds (n=10) were selected randomly for sample collection. Five birds were selected from Government sector and five from private breeding farms. DNA was extracted, purified and measured by using Nano drop. Extracted DNA was amplified using universal primers targeting Cytochrome b gene on polymerase chain reaction (PCR). PCR product was run on a gel for the desired DNA bands. DNA from gel was eluted and sent for sequencing. The sequences were compared with a reference reported sequence of cyt b gene of Indian peafowl with Accession No. L08379.1to find out the genetic diversity. Indian peafowl of government sector showed more similarity ³95% rather than bird of private sector with ³90% homology with reference Accession No. More genetic variation, which is the guarantee of resistance to disease and environmental fitness among the Indian peafowl at private sector, might be due to random reproductive behavior.  

REFERENCES


  1. Alonso, P.D., Milner, A.C., Ketcham, R.A., Cookson, M.J. and Rowe, T.B. (2004). The avian nature of the brain and inner ear of Archaeopteryx. Nature., 430: 666-669.

  2. Arnold, A.E., Andersen, E.M., Taylor, M.J. and Steidl, R.J. (2017). Using cytochrome b to identify nests and museum specimens of cryptic songbirds. Conserv. Genet. Res.,20: 1-8. 

  3. Baig, K. (2006). Environmental baseline survey and monitoring of Taunsa Barrage Emergency Rehabilitation and Modernisation Project: A report submitted to Zoological Science Department, Pakistan Museum of Natural History. Pakistan Museum of Natural history. 22.

  4. Boonkhaw, P., Prayoon, U., Kanchanasaka, B., Hayashi, F. and Tamura, N. (2017). Colour polymorphism and genetic relationships among twelve subspecies of Callosciurus finlaysonii in Thailand. Mam. Biol.,85: 6-13.

  5. Briolay, J., Galtier, N., Brito, R.M. and Bouvet, Y. (1998). Molecular phylogeny of cyprinidae inferred fromcytochrome bDNA Sequences. Mol. Phylogenet. Evol., 9: 100-108.

  6. Brown, J.W. and Van Tuinen, M. (2011). 12 Evolving Perceptions on the Antiquity of the Modern Avian Tree. Living Dinosaurs: The Evolutionary History of Modern Birds. 306.

  7. Clayton, D.H., Koop, J.A., Harbison, C.W., Moyer, B.R. and Bush, S.E. (2010). How birds combat ectoparasites. Open Ornithol. J. 3: 41-71.

  8. Dash, S., Kumarasamy, P., Malik, H. and Thiagarajan, V. (2013). Phylogenetic analysis of ratite bird emu (Dromaius Novaehollandiae) using sex specific sequences. Indian J. Anim. Res.,47: 247-250.

  9. Del-Hoyo, J., Elliott, A. and Sargatal, J. (1999). Handbook of the Birds of the World, vol. 5. Lynx Edicions, Barcelona. pp. 15-25.

  10. Jain, D. and Rana, S. (2013). Population indices and habitat association of Indian Peafowl (Pavo cristatus) in Haryana using line transect and call count method. Indian J. Anim. Res.,47: 152-155.

  11. Klaassen, M. (1996). Metabolic constraints on long-distance migration in birds. J. Exp. Biol.,199: 57-64.

  12. Koju, N.P., He, K., Chalise, M.K., Ray, C., Chen, Z., Zhang, B., Wan, T., Chen, S. and Jiang, X. (2017). Multilocus approaches reveal underestimated species diversity and inter-specific gene flow in pikas (Ochotona) from southwestern China. Mol. Phylogenet. Evol.,107: 239-245.

  13. Krebs, C. (1998). Benjamin/Cummings. Ecological Methodology. 2nd edition. Menlo Park, CA.

  14. Livezey, B.C. and Zusi, R.L. (2007). Higher order phylogeny of modern birds (Theropoda, Aves: Neornithes) based on comparative anatomy. II. Analysis and discussion. Zool. J. Linnean Soc.,149: 1-95.

  15. Mouritsen, H. and Larsen, O.N. (2001). Migrating songbirds tested in computer-controlled Emlen funnels use stellar cues for a time-    independent compass. J. Exp. Biol.,204: 3855-3865.

  16. Podsiadlowski, L., Gamauf, A. and Töpfer, T. (2017). Revising the phylogenetic position of the extinct Mascarene Parrot Mascarinus mascarin (Linnaeus 1771)(Aves: Psittaciformes: Psittacidae). Mol. Phylogenet. Evol.,107: 499-502.

  17. Rana, S. and Jain, D. (2013). Impact of modern agricultural practices on population density of Indian peafowl (Pavo cristatus) in Haryana, India. Agri. Sci. Digest. 33.

  18. Saikia, J., Roy, T., Naskar, S., Das, B. and Ferdoci, A. (2015). Molecular characterization of Cytochrome B gene in indigenous pig. Indian. J. Anim. Res. 49: 196-198.

  19. Simons, M.J., Winney, I., Nakagawa, S., Burke, T. and Schroeder, J. (2015). Limited catching bias in a wild population of birds with near complete census information. Ecol. Evol.,5: 3500-3506.

  20. Vincze, O. (2016). Light enough to travel or wise enough to stay? Brain size evolution and migratory behavior in birds. Evol.,70: 2123-2133.

  21. Wilson Jr, W.H. (1999). Bird feeding and irruptions of northern finches: Are migrations short-stopped. North American Bird Bander. 24:17-23.

  22. Zhou, T.-C., Sha, T., Irwin, D.M. and Zhang, Y.-P. (2015). Complete mitochondrial genome of the Indian peafowl (Pavo cristatus), with phylogenetic analysis in phasianidae. Mit. DNA.,26: 912-913. 
Reviewed By

Download Article
In this Article
    Contact us
    Follow us

    Editorial Board

    View all (0)