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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 7 (july 2019) : 860-863,   Doi: 10.18805/ijar.B-723

SRY and DelE Detection in Polled Intersex Tangshan Dairy Goat

Y
Y. He
Y
Y. Zhang
X
X. Li
R
R. Zhou
L
L. Li
Z
Z. Wang
1Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, China.
Cite article:- He Y., Zhang Y., Li X., Zhou R., Li L., Wang Z. (2017). SRY and DelE Detection in Polled Intersex Tangshan Dairy Goat. Indian Journal of Animal Research. 53(7): 860-863. doi: 10.18805/ijar.B-723.

ABSTRACT

The sex determining region Y (SRY) gene and deletion element DelE were analyzed in 34 polled intersex syndrome (PIS) or 6 normal Tangshan dairy goats (3 females and 3 males) by PCR technology. The result showed that the special male SRY gene only existed in male goats, and was absent in all PIS goats or normal female goats. The 28 PIS goats were homozygote for DelE, and the other 6 goats were heterozygote for DelE deletion or homozygote for non DelE deletion. It could be concluded that the PIS Tangshan dairy goats were female for its genetic basis and the DelE was partial deletion. 

REFERENCES

  1. Asdell, S (1944). The genetic sex of intersexual goats and a probable linkage with the gene for hornlessness. Science, 99:124-129.
  2. Batista, M; Gonzale F; Cabrera, F; Palomino, E; Castellano, E; Calero, P; Gracia, A (2000). True hermaphroditism in a horned goat with 6OXX/6OXY chimerism. Canadian Veterinary Journal, 41:562–564.
  3. Boulanger, L., Kocer, A., Daniel, N., Pannetier, M, Chesné, P., Heyman, Y., Renault, L., Mandon-Pépin, B., Chavatte-Palmer, P., Vignon, X., Vilotte, J.L., Cotinot, C., Renard, J.P., Pailhoux, E. (2008). Attempt to rescue sex-reversal by transgenic expression of the PISRT1 gene in XX PIS-/- goats. Sex Dev. 2:142-51. doi: 10.1159/000143432.
  4. Chang, H (1980). Genetics analysis of intersex goats. Acta Veterinaria et Zootechnica Sinica, 11:245-250.
  5. E G, Huang Y, Zhao Y, Na R, Zhao Z, Sun Y, Chen L and Qiu X(2015). Comparative analysis the variability of a complete mitochondrial genome of polledness intersexual goat (PIS- -) from China. Indian J. Anim. Res., 49 (6):761-763.
  6. Jager, RJ; Anvret, M; Hall, K; Scherer, G; (1990). A human XY female with a frame shift mutation in the candidate testis-determining gene SRY. Nature, 29:452–4.
  7. Koopman, P; Gubbay, J; Vivian, N; Goodfellow, P; Lovell-Badge, R (1991). Male development of chromosomally female mice transgenic for Sry. Nature, 351:117–121.
  8. Konnai, S; Imamura, S; Nakajima, C; Witola, WH; Yamada, S; Simuunza, M; Nambota, A;et al (2006). Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus. Acta Trop, 99: 34–41.
  9. Maier, EM; Leitner, C; Lohrs, U; Kuhnle, U (2003). True hermaphroditism in an XY individual due to a familial point mutation of the SRY gene. J Pediatr Endocrinol Metab, 16:575–80.
  10. Monteagudo, LV; Arruga, MV; Bonafonte, JI; Ordás, M et al. (2008). Bilateral Leydig cell tumor in a six-year-old intersex goat affected by Polled Intersex Syndrome. Vet Pathol, 45–42.
  11. Mukherjee A, De S, Verma A, Dass G and Chakraborty D.( 2015).Prediction of structure and functions from full length coding sequence of SRYgene in crossbred and Indicine bulls. Indian J. Anim. Res., 49 (2): 180-186.
  12. Pailhoux, E; Vigier, B; Chaffaux, S; Servel, N; Taourit, S; Furet, JP; Fellous, M et al (2001). A 11.7-kb deletion triggers intersexuality and polledness in goats. Narure, 29:453–458. 
  13. Pailhoux, E; Vigier, B; Vaiman, D; Servel, N; Chaffaux, SP; Edmond, P; Cribiu; Cotinot, C (2002). Ontogenesis of Female-to-Male Sex-Reversal in XX Polled Goats. Developmental Dynamics, 24:39–50. 
  14. Pailhoux, E., Vigier, B., Schibler, L., Cribiu, E.P., Cotinot, C., and Vaiman, D. (2005). Positional cloning of the PIS mutation in goats and its impact on understanding mammalian sex-differentiation. Genet Sel Evol. 37: 1:S55-64.
  15. Shinichi, Y; Aya, M; Takehito, M; Takeshi, T; Mitsugu, H; Saburo, M; Yoshiaki, H (2011). Polled intersex syndrome with urethral atresia in a goat. Internal Medicine, 7:1355-1357. 
  16. Vaiman, D; Schibler, L; Oustry-Vaiman, A; Pailhoux, E; Goldammer, T; Stevanovic, M et al. (1999). High-resolution Human/Goat comparative map of the goat Polled/Intersex syndrome (PIS): The human homologue is contained in a human YAC from HSA3q23. Genomics, 56:31–39. 
  17. Yang, B; Ja, L; Zhao, D (2012). Detection and application of PIS genetic deficiency gene in dairy goat. Hereditas, 34:895-900.
  18. Zhang, LJ; Luo, J; Wu, HJ (2006). The research development of intersex goat. China herbivores, 26:48–50.
  19. Zhang, H., Duan, X.Y., Li, H.J., Niu, L.L., Wang, L.J., Li, L. , Zhang, H.P. and Zhong, T. (2014). The complete mitochondrial genome of Chinese Tibetan goat (Capra hircus). Mitochondrial DNA. doi: 10.3109/19401736. 2014.936418. 
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Indian Journal of Animal Research
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    Research Article

    volume 53 issue 7 (july 2019) : 860-863,   Doi: 10.18805/ijar.B-723

    SRY and DelE Detection in Polled Intersex Tangshan Dairy Goat

    Y
    Y. He
    Y
    Y. Zhang
    X
    X. Li
    R
    R. Zhou
    L
    L. Li
    Z
    Z. Wang
    1Department of Animal Science, Hebei Normal University of Science and Technology, Qinhuangdao, 066004, China.
    Cite article:- He Y., Zhang Y., Li X., Zhou R., Li L., Wang Z. (2017). SRY and DelE Detection in Polled Intersex Tangshan Dairy Goat. Indian Journal of Animal Research. 53(7): 860-863. doi: 10.18805/ijar.B-723.

    ABSTRACT

    The sex determining region Y (SRY) gene and deletion element DelE were analyzed in 34 polled intersex syndrome (PIS) or 6 normal Tangshan dairy goats (3 females and 3 males) by PCR technology. The result showed that the special male SRY gene only existed in male goats, and was absent in all PIS goats or normal female goats. The 28 PIS goats were homozygote for DelE, and the other 6 goats were heterozygote for DelE deletion or homozygote for non DelE deletion. It could be concluded that the PIS Tangshan dairy goats were female for its genetic basis and the DelE was partial deletion. 

    REFERENCES

    1. Asdell, S (1944). The genetic sex of intersexual goats and a probable linkage with the gene for hornlessness. Science, 99:124-129.
    2. Batista, M; Gonzale F; Cabrera, F; Palomino, E; Castellano, E; Calero, P; Gracia, A (2000). True hermaphroditism in a horned goat with 6OXX/6OXY chimerism. Canadian Veterinary Journal, 41:562–564.
    3. Boulanger, L., Kocer, A., Daniel, N., Pannetier, M, Chesné, P., Heyman, Y., Renault, L., Mandon-Pépin, B., Chavatte-Palmer, P., Vignon, X., Vilotte, J.L., Cotinot, C., Renard, J.P., Pailhoux, E. (2008). Attempt to rescue sex-reversal by transgenic expression of the PISRT1 gene in XX PIS-/- goats. Sex Dev. 2:142-51. doi: 10.1159/000143432.
    4. Chang, H (1980). Genetics analysis of intersex goats. Acta Veterinaria et Zootechnica Sinica, 11:245-250.
    5. E G, Huang Y, Zhao Y, Na R, Zhao Z, Sun Y, Chen L and Qiu X(2015). Comparative analysis the variability of a complete mitochondrial genome of polledness intersexual goat (PIS- -) from China. Indian J. Anim. Res., 49 (6):761-763.
    6. Jager, RJ; Anvret, M; Hall, K; Scherer, G; (1990). A human XY female with a frame shift mutation in the candidate testis-determining gene SRY. Nature, 29:452–4.
    7. Koopman, P; Gubbay, J; Vivian, N; Goodfellow, P; Lovell-Badge, R (1991). Male development of chromosomally female mice transgenic for Sry. Nature, 351:117–121.
    8. Konnai, S; Imamura, S; Nakajima, C; Witola, WH; Yamada, S; Simuunza, M; Nambota, A;et al (2006). Acquisition and transmission of Theileria parva by vector tick, Rhipicephalus appendiculatus. Acta Trop, 99: 34–41.
    9. Maier, EM; Leitner, C; Lohrs, U; Kuhnle, U (2003). True hermaphroditism in an XY individual due to a familial point mutation of the SRY gene. J Pediatr Endocrinol Metab, 16:575–80.
    10. Monteagudo, LV; Arruga, MV; Bonafonte, JI; Ordás, M et al. (2008). Bilateral Leydig cell tumor in a six-year-old intersex goat affected by Polled Intersex Syndrome. Vet Pathol, 45–42.
    11. Mukherjee A, De S, Verma A, Dass G and Chakraborty D.( 2015).Prediction of structure and functions from full length coding sequence of SRYgene in crossbred and Indicine bulls. Indian J. Anim. Res., 49 (2): 180-186.
    12. Pailhoux, E; Vigier, B; Chaffaux, S; Servel, N; Taourit, S; Furet, JP; Fellous, M et al (2001). A 11.7-kb deletion triggers intersexuality and polledness in goats. Narure, 29:453–458. 
    13. Pailhoux, E; Vigier, B; Vaiman, D; Servel, N; Chaffaux, SP; Edmond, P; Cribiu; Cotinot, C (2002). Ontogenesis of Female-to-Male Sex-Reversal in XX Polled Goats. Developmental Dynamics, 24:39–50. 
    14. Pailhoux, E., Vigier, B., Schibler, L., Cribiu, E.P., Cotinot, C., and Vaiman, D. (2005). Positional cloning of the PIS mutation in goats and its impact on understanding mammalian sex-differentiation. Genet Sel Evol. 37: 1:S55-64.
    15. Shinichi, Y; Aya, M; Takehito, M; Takeshi, T; Mitsugu, H; Saburo, M; Yoshiaki, H (2011). Polled intersex syndrome with urethral atresia in a goat. Internal Medicine, 7:1355-1357. 
    16. Vaiman, D; Schibler, L; Oustry-Vaiman, A; Pailhoux, E; Goldammer, T; Stevanovic, M et al. (1999). High-resolution Human/Goat comparative map of the goat Polled/Intersex syndrome (PIS): The human homologue is contained in a human YAC from HSA3q23. Genomics, 56:31–39. 
    17. Yang, B; Ja, L; Zhao, D (2012). Detection and application of PIS genetic deficiency gene in dairy goat. Hereditas, 34:895-900.
    18. Zhang, LJ; Luo, J; Wu, HJ (2006). The research development of intersex goat. China herbivores, 26:48–50.
    19. Zhang, H., Duan, X.Y., Li, H.J., Niu, L.L., Wang, L.J., Li, L. , Zhang, H.P. and Zhong, T. (2014). The complete mitochondrial genome of Chinese Tibetan goat (Capra hircus). Mitochondrial DNA. doi: 10.3109/19401736. 2014.936418. 
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