Indian Journal of Animal Research

  • Chief EditorK.M.L. Pathak

  • Print ISSN 0367-6722

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Indian Journal of Animal Research, volume 52 issue 2 (february 2018) : 296-300

Antimicrobial sensitivity of pathogens causing subclinical mastitis in goats in Bulgaria

Kalin Hristov
1<p>University of Forestry, Faculty Veterinary Medicine,&nbsp;10 Kliment Ohridsky Blvd., Sofia 1756, Bulgaria</p>
Cite article:- Hristov Kalin (2017). Antimicrobial sensitivity of pathogens causing subclinical mastitis in goats in Bulgaria . Indian Journal of Animal Research. 52(2): 296-300. doi: 10.18805/ijar.v0iOF.8497.

The aim of this study was to analyze the antibacterial sensitivity of microbial isolates causing subclinical mastitis in goats. The classical Bauer disk-diffusion assay was used to determine the sensitivity of the coagulase-negative staphylococcal (CNS) isolates and isolates belonging to other genera. In all the tested isolates, the highest rates of resistance were observed to be to Oxacillin (81.25%) and Novobiocin (75%), followed by Penicillin (68.75%), Tetracycline (37.5%) and Amoxycillin (31.25%). There was low prevalence of resistance to Cefotaxime (12.5%), Thiamphenicol (6.25%), Cefuroxim (6.25%), Cefuroxim (6.25%), Amikacin (6.25%), Sulfamethoxazole + Ttrimethoprim (6.25%) and Gentamicin (6.25%). Staph. intermedius showed resistance to the highest number of antimicrobial agents, four ones (28.57%), whereas Staph. haemolyticus was the least resistant isolate, which was sensitive to nearly all of the tested antimicrobials and showed intermediate sensitivity to only three ones (21.43%). Among the microorganisms other than CNS, B.brevis and Å.coli showed resistance to the highest number of antimicrobial agents (42.86%). 


  1. Bauer, A.W., Kirby., W.M., Sherris J.C. and Turck M. (1966). Antibiotic susceptibility testing by a standardized single disk method. American Journal of Clinical Pathology., 45 (4): 493-496

  2. Bhujbal, J., Deshumukh, V., Abdul. A., Gujar. M. and Aziz A. (1999). Goat mastitis microflora and antibiogram. Indian Journal of Comparative Microbiology, Immunology and Infectious Diseases., 20: 44-46

  3. Bochev, I. and Russenova N. (2005). Resistance of Staphylococcus spp. strains isolated from goat with sub clinical mastitis. Bulgarian Journal of Veterinary Medicine., 8: 109–118. 

  4. Contreras, A., Corrales. J.C., Sierra., D., Marco, J., and Sanchez A. (1997). Persistence of subclinical intramammary pathogens in goats throughout lactation. J Dairy Sci., 80: 2815–2819.

  5. Contreras, A., Corrales., J.C., Sierra D. and Marco J. (1995). Prevalence and aetiology of non-clinical intramammary infection in Murciano-Granadina goats. Small Rumin Res., 17:71–78.

  6. Contreras, A., Paape M.J. and Miller R.H. (1999). Prevalence of subclinical intramammary infection caused by Staphylococcus epidermidis in a commercial dairy goat herd. Small Rumin Res. 31 :203–208.

  7. Mhase, P.P., Harne, S.D., and Swyawamhi. P.R. (2007). Antibiogram of Aerobic Bacteria Associated with Goat Mastitis. J. Bombay Vel. Coll., 15:52 

  8. Mishra, P., Sidhartha, H., Pal, A., and Hazari S. (1996). Subclinical mastitis in goat with special reference to fungus. Indian Journal of Dairy Science., 49 (3): 209-210.

  9. Moroni, P., Vellere. F., Antonini. M., Pisoni. G., Ruffo, G., and Carli S. (2004). Antibiotic susceptibility of coagulase-    negativestaphylococci isolated from goats’ milk. International Journal of Antimicrobial Agents., 23 (6): 637–640. 

  10. Popova, T. and Kanchev K. (2013). Microflora of internal organs and muscles of lambsand pigs in spontaneous infection with Cysticercus tenuicollis. Bulgarian Journal of Agricultural Science,., 19 (2): 325-330. 

  11. Parvanov, P. and Popova T.(2000). Drug sensitivity of microorganisms causing subclinical mastitis in buffaloes. Bulg. J. Agric. Sci., 6 (1): 111–118.

  12. Popova, T. and N. Dimitrov. (2000). In vitro testing of antibacterial activity of amphenicols. Bulg. J. Agric. Sci., 6 (6): 711-716.

  13. Popova, T. and. Dimitrov N. (2001). Florfenicol and thiamphenicol - perspective analogs of chloramphenicol. Bulg. J. Agric. Sci., 7 (1): 95-100.

  14. Popova, T. and Todorov T. (2008). In vitro antimicrobial activity of enrofloxacin against clinical isolates from dogs and cats in Bulgaria. Folia Veterinaria., 52 (3-4): 124-128. 

  15. Ryan, D.P. and Greenwood. P.L. (1990). Prevalence of udder bacteria in milk sample from four dairy goat herds. Aust Vet J.. 67: 362–    366.

  16. Scheer, M. (1987). Studies on the antibacterial activity of Baytril. Veterinary Medical Review., 2: 90-99. 

  17. Virdis, S., Scarano. C., Cossu. F., Spanu. V., Spanu C., and Luigi De Santis E.P. (2010). Antibiotic Resistance in Staphylococcus aureus and Coagulase negative Staphylococci isolated from goats with subclinical mastitis. Veterinary Medicine International. Vol. 2010., doi:10.4061/2010/517060

  18. White, E.C. and Hinckley L.S.. (1999). Prevalence of mastitis pathogens in goat milk. Small Rumin Res., 33: 117–21.

  19. Yordanov, S., Minkova. A., Popova. T., Dimitrova, A., and Gantchev K. (2004). Susceptibility to some antibacterial means of Staphylococcus isolated from dogs and cats. Veterinary Medicine., 8 (1-2): 29 -32.

     

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