Published In
Indian Journal of Animal Research
Article Metrics

0
Views
0
Citations
Reviewed By
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
Follow us
Research Article
volume 52 issue 8 (august 2018) : 1223-1226, Doi: 10.18805/ijar.B-688
Histological description of aceh cattle ovary cryopreserved by various cryoprotectants
1Laboratory of Clinic, Veterinary Medicine Faculty, Syiah Kuala University, Banda Aceh, Indonesia.
Submitted07-01-2017|
Accepted19-02-2018|
First Online 22-08-2018|
Cite article:- Syafruddin Syafruddin, Siregar Nizwan Tongku, Azrina Azrina, Armansyah Teuku, Panjaitan Budianto, Aliza Dwinna, Sutriana Amalia, Zuhrawati Zuhrawati, Rosmaidar Rosmaidar, Roslizawaty Roslizawaty (2018). Histological description of aceh cattle ovary cryopreserved by various cryoprotectants. Indian Journal of Animal Research. 52(8): 1223-1226. doi: 10.18805/ijar.B-688.
ABSTRACT
This research aimed to determine Aceh cattle ovarian follicle morphological integrity after vitrified by various cryoprotectants. Cryoprotectants used in this research were 30% ethylene glycol (EG), 30% dimethyl suphocide (DMSO), and combination of 15% EG + 15% DMSO. Prior to vitrification process, ovaries were cleansed by phosphate buffered saline (PBS) and then cut into ±1 mm³ . Ovaries were consecutively submerged into the following liquid for 5 minutes each: PBS+ 0.25 M sucrose; PBS+ 0.5 M sucrose; PBS+ 0.5 M sucrose + 10% cryoprotectants; and PBS+ 0.5 M sucrose + 30% cryoprotectants. Then, ovaries were packed into straws with ±7 cm in length and ± 6 mm in diameter. Before kept in liquid nitrogen, ovaries were first exposed to nitrogen fume for 10 second. After being stored for 1 day, the ovaries were proceed for histological examination . The result showed that Aceh cattle ovarian follicle after vitrification using 30% EG yields the best morphological integrity. Cumulus oophorus, zona pellucida, granulose cell arrangement, theca interna, and theca externa cells were observed clearer in ovary which was vitrified with 30 % EG than those with 30% DMSO and combination of 15% EG + 15% DMSO. As conclusion, 30% EG was able to protect ovary morphological integrity better than 15 % EG + 15% DMSO and 30% DMSO. Furthermore, combination of 15% EG+ 15 % DMSO was relatively better in protecting ovary follicle morphological integrity compared to 30% DMSO.
REFERENCES
- Dattena M, Accardo C, Pilichi S. (2004). Comparison of different vitrification protocols on viability after transfer of ovine blastocysts in vitro produced and in vivo derived. Theriogenology. 62: 481-493.
- Djuwita I, Boediono A, Sukra Y.(2000). Morphology and fertilization rate of vitrified ovine oocytes matured in vitro. 14th International Congress on Animal Reproduction, Stockholm. 2: 202-207.
- Donnez J, Dolmans MM, Squifflet J. (2006). Restoration of ovarian function after orthotopic (intraovarian and priovarian) transplantationof cryopreserved ovarian tissue in a women treated by bone marrow transplantation for sickle cell anemia. Hum Reprod. 21: 183-188.
- Fukui EJ, Xia L, Downey BR. (1993). Ultrastructural changes in bovine oocyte cryopreserved by vitrification. Cryobiology. 32: 139- 156.
- Gao D, Critser JK. (2000). Mechanisms of cryoinjury in living cells. ILARJ. 41: 187-196.
- Ghorbani M, Sadrkhanlou R, Nejati V, Ahmadi A, Tizroo G. (2012). The effects of dimethyl sulfoxide and ethylene glycol as vitrification protectants on different cleavage stages of mouse embryo quality. Vet Res Forum. 3: 245-249.
- Hyttel P, Fair T, Greve T. (1997). Oocyte growth, capacitation and final maturation in cattle. Theriogenology. 47: 23-32.
- Jain JK, Paulson RJ. (2006). Oocyte cryopreservation. Fertil Steril. 86: 1037-1046.
- Kasai M. (2002). Advances in the cryopreservation of mammalian oocytes and embryos: Development of ultra rapidvitrification. Reprod Med Biol. 1: 1-9.
- Lucci CM, Kacinskis MA, Rumpf R. (2004). Effect of different cryoprotectants on the structural preservation of follicles in frozen zebu bovine (Bosindicus) ovarian tissue. Theriogenology. 61: 1101-1114.
- Lucci CM, Schreier LL, Machado GM. (2007) Effects of storing pig ovaries at 4 or 20°C for different periods of time on the morphology and viability of pre-antral follicles. Reprod Dom Anim. 42: 76-82.
- Mohamad K, Rumiyati E, Sari F. (2000). Kriopreservasi Oosit pronukleus dan embrio mencit dengan metode vitrifikasi. Seminar Nasional Biologi XVI Bandung. 45-49.
- Mohamad K, Djuwita I, Boediono A. (2005). Vitrifikasi ovarium mencit menggunakan etilenglikol dan DMSO sebagai krioprotektan dan viabilitasnya pasca auto transplantasi di subkapsulaginjal. Med Ked Hewan. 21: 23-27.
- Rosadi B, Setiadi MA, Sajuthi D. (2011). Presevasi ovarium dan pengaruhnya terhadap morfologi folikel domba. J. Vet. 12: 11-15.
- Takeda T. (1990). Preparing Cryoprotectants. In Techniques for Freezing Mammalian Embryos. Seidel, G.E. (Ed.). Colorado State University, USA. 97-102.
- Wahjuningsih S, Harjopranjoto S, Sumitro SB. (2010). Pengaruh konsentrasi etilen glikol dan lama paparan terhadap tingkat fertilitas in vitro oosit sapi. J Ked Hewan. 4: 12-17.
- Vajta G , Im PH, Kuwayama M. (1998). Open pulled straw (OPS) vitrification: A new way to reduce cryo injuries of bovine ova and embryos. Mol Reprod Dev. 51: 53-58.
Disclaimer :
All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers. Any product that may be evaluated in this article or claim that may be made by its manufacturer is not guaranteed or endorsed by the publisher.
Copyright :
This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
In this Article
APC
APC cover the cost of turning a manuscript into a published manuscript through peer-review process, editorial work as well as the cost of hosting, distributing, indexing and promoting the manuscript.
Publish With US
Submit your manuscript through user friendly platform and acquire the maximum impact for your research by publishing with ARCC Journals.
Become a Reviewer/Member
Join our esteemed reviewers panel and become an editorial board member with international experts in the domain of numerous specializations.
Open Access
Filling the gap between research and communication ARCC provide Open Access of all journals which empower research community in all the ways which is accessible to all.
Products and Services
We provide prime quality of services to assist you select right product of your requirement.
Support and Policies
Finest policies are designed to ensure world class support to our authors, members and readers. Our efficient team provides best possible support for you.
Follow us
Published In
Indian Journal of Animal Research