Histological description of aceh cattle ovary cryopreserved by various cryoprotectants

DOI: 10.18805/ijar.B-688    | Article Id: B-688 | Page : 1223-1226
Citation :- Histological description of aceh cattle ovary cryopreserved by various cryoprotectants.Indian Journal of Animal Research.2018.(52):1223-1226
Syafruddin Syafruddin, Tongku Nizwan Siregar, Azrina Azrina, Teuku Armansyah, Budianto Panjaitan, Dwinna Aliza , Amalia Sutriana, Zuhrawati Zuhrawati, Rosmaidar Rosmaidar, Roslizawaty Roslizawaty roslizakh@unsyiah.ac.id
Address : Laboratory of Clinic, Veterinary Medicine Faculty, Syiah Kuala University, Banda Aceh, Indonesia.
Submitted Date : 7-01-2017
Accepted Date : 19-02-2018


This research aimed to determine Aceh cattle ovarian follicle morphological integrity after vitrified by various cryoprotectants. Cryoprotectants used in this research were 30% ethylene glycol (EG), 30% dimethyl suphocide (DMSO), and combination of 15% EG + 15% DMSO. Prior to vitrification process, ovaries were cleansed by phosphate buffered saline (PBS) and then cut into ±1 mm³ . Ovaries were consecutively submerged into the following liquid for 5 minutes each: PBS+ 0.25 M sucrose; PBS+ 0.5 M sucrose; PBS+ 0.5 M sucrose + 10% cryoprotectants; and PBS+ 0.5 M sucrose + 30% cryoprotectants. Then, ovaries were packed into straws with ±7 cm in length and ± 6 mm in diameter. Before kept in liquid nitrogen, ovaries were first exposed to nitrogen fume for 10 second. After being stored for 1 day, the ovaries were proceed for histological examination . The result showed that Aceh cattle ovarian follicle after vitrification using 30% EG  yields the best morphological integrity. Cumulus oophorus, zona pellucida, granulose cell arrangement, theca interna, and theca externa cells were observed clearer in ovary which was vitrified with 30 % EG than those with 30% DMSO  and combination of 15% EG + 15% DMSO. As conclusion, 30% EG  was able to protect ovary morphological integrity better than 15 % EG + 15% DMSO  and 30% DMSO. Furthermore, combination of 15% EG+ 15 % DMSO  was relatively better in protecting ovary follicle morphological integrity compared to 30% DMSO.


Aceh cattle Cryoprotectant Ovary Vitrification.


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