Evaluation of acute toxicity and effects of sub-acute concentrations of copper oxide nanoparticles (CuO-NPs) on hematology, selected enzymes and histopathology of liver and kidney in Mus musculus

DOI: 10.18805/ijar.v0iOF.8489    | Article Id: B-683 | Page : 92-98
Citation :- Evaluation of acute toxicity and effects of sub-acute concentrations of copper oxide nanoparticles (CuO-NPs) on hematology, selected enzymes and histopathology of liver and kidney in Mus musculus .Indian Journal of Animal Research.2018.(52):92-98

Atif Yaqub, Khalid Mahmood Anjum, Amnah Munir, Hamid Mukhtar and Waseem Ahmad Khan

Address :

Department of Zoology, Government College University, Lahore-Pakistan

Submitted Date : 6-01-2017
Accepted Date : 31-03-2017


Industrial use of nanoparticles and their accumulation during the recent decade have created an urgent need to assess their environmental implications. The current study deals with the evaluation of acute toxicity of copper oxide nanoparticles (CuO-NPs) in the albino mice (Mus musculus). Lethal dose of these nanoparticles in albino mice injected via intravenous route were found to be 550 mg/kg body weight (BW). Exposure of the albino mice to sub-lethal concentrations of these nanoparticles resulted in altered hematological parameters such as a significant increase in white blood cells (WBCs), a significant decrease in red blood cells (RBCs), hemoglobin (Hb) and platelets count. NPs significantly elevated the levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), urea and creatinine. Histopathological examination of liver and kidney showed that sub-lethal doses of CuO-NPs, in liver, led to rupture of hepatocytes, dilation of sinusoid space, hemorrhaging in hepatic tissues, and congestion of the central vein with red blood cells leading towards ultimate rupture. On the other hand, the kidney showed ruptured renal capsule, loss of urinary space, swelling in glomerulus, degeneration in podocysts, and cytoplasmic vacuolization


Acute toxicity Metal oxide nanoparticles Mus musculus Nanotechnology. Rodents.


  1. Abdelhalim, M.A.K. (2011). Exposure to gold nanoparticles produces cardiac tissue damage that depends on the size and duration of exposure. Lipids Health Dis., 10: 1-6.
  2. Afifi, M., Zinada, O.A.A., Ali, H. and Couderchet, M. (2016). Zinc nanoparticles induced brain lesions and behavioral changes in Tilapia nilotica and Tilapia zillii. Indian J. Anim. Res., 50: 764-768.
  3. Anjum, K., Mughal, M., Sayyed, U., Yaqub, A., Khalique, A., Rashid, M., Yousaf, M. and Mumtaz, N. (2014). Iinfluence of increasing fluoride dose rates on selected liver and kidney enzymes profile in domestic chicken (Gallus domesticus). J. Anim. Plant Sci., 24: 77-80.
  4. Ayaz, N.O., Ramadan, K.S., Farid, H.E. and Alnahdi, H.S. (2016). Protective role and antioxidant activity of arabic gum against trichloroacetate-induced toxicity in liver of male rats. Indian J. Anim. Res. (Online First).
  5. Begley, C.G. and Ellis, L.M. (2012). Drug development: Raise standards for preclinical cancer research. Nature., 483: 531-533.
  6. Burnett, M.E. and Wang, S.Q. (2011). Current sunscreen controversies: a critical review. Photoderm. Photoimmuno.Photomed., 27: 58-67.
  7. Cava, R. (1990). Structural chemistry and the local charge picture of copper oxide superconductors. Sci., 247: 656-662.
  8. Chen, Z., Meng, H., Xing, G., Chen, C., Zhao, Y., Jia, G., Wang, T., Yuan, H., Ye, C. and Zhao, F. (2006). Acute toxicological effects of copper nanoparticles in vivo. Toxicology letters., 163: 109-120.
  9. Griffitt, R.J., Weil, R., Hyndman, K.A., Denslow, N.D., Powers, K., Taylor, D. and Barber, D.S. (2007). Exposure to copper nanoparticles causes gill injury and acute lethality in zebrafish (Danio rerio). Environ. Sci. Tech., 41: 8178-8186.
  10. Khabbazi, M., Harsij, M., Hedayati, S.A.A., Gholipoor, H., Gerami, M.H. and Ghafari Farsani, H. (2015). Effect of CuO nanoparticles on some hematological indices of rainbow trout Oncorhynchus mykiss and their potential toxicity. Nanomed. J., 2: 67-73.
  11. Li, S.-Q., Zhu, R.-R., Zhu, H., Xue, M., Sun, X.-Y., Yao, S.-D. and Wang, S.-L. (2008). Nanotoxicity of TiO 2 nanoparticles to erythrocyte in vitro. Food Chem.Toxicol., 46: 3626-3631.
  12. Ma, L., Zhao, J., Wang, J., Liu, J., Duan, Y., Liu, H., Li, N., Yan, J., Ruan, J. and Wang, H. (2009). The acute liver injury in mice caused by nano-anatase TiO 2. Nanoscale Res.Letters., 4: 1275-1282.
  13. Melegari, S.P., Perreault, F., Costa, R.H.R., Popovic, R. and Matias, W.G. (2013). Evaluation of toxicity and oxidative stress induced by copper oxide nanoparticles in the green alga Chlamydomonas reinhardtii. Aqua.Toxicol., 142: 431-440.
  14. Sampson, E.J., Whitner, V.S., Burtis, C.A., McKneally, S., Fast, D.M. and Bayse, D.D. (1980). An interlaboratory evaluation of the IFCC method for aspartate aminotransferase with use of purified enzyme materials. Cli.Chem., 26: 1156-1164.
  15. Sangha, G. and Kaur, K. (2011). Cypermethrin induced changes in biochemical constituents of pl constituents of plasma of female albino rats. Indian J. Anim. Res., 45: 186-191.
  16. Singh, N., Manshian, B., Jenkins, G.J., Griffiths, S.M., Williams, P.M., Maffeis, T.G., Wright, C.J. and Doak, S.H. (2009). NanoGenotoxicology: the DNA damaging potential of engineered nanomaterials. Biomaterial., 30: 3891-3914.
  17. Tang, J., Xiong, L., Wang, S., Wang, J., Liu, L., Li, J., Yuan, F. and Xi, T. (2009). Distribution, translocation and accumulation of silver nanoparticles in rats. J. Nanosci.Nanotech., 9: 4924-4932.
  18. Wang, B., Feng, W.-Y., Wang, T.-C., Jia, G., Wang, M., Shi, J.-W., Zhang, F., Zhao, Y.-L. and Chai, Z.-F. (2006). Acute toxicity of nano-and micro-scale zinc powder in healthy adult mice. Toxicol.Letter., 161: 115-123.
  19. Wang, J.-X., Fan, Y.-B., Gao, Y., Hu, Q.-H. and Wang, T.-C. (2009). TiO 2 nanoparticles translocation and potential toxicological effect in rats after intraarticular injection. Biomaterial., 30: 4590-4600.
  20. Xu, P., Xu, J., Liu, S. and Yang, Z. (2012). Nano copper induced apoptosis in podocytes via increasing oxidative stress. J.Hazar.Material., 241: 279-286.
  21. Zhang, X.-D., Wu, H.-Y., Wu, D., Wang, Y.-Y., Chang, J.-H., Zhai, Z.-B., Meng, A.-M., Liu, P.-X., Zhang, L.-A. and Fan, F.-Y. (2010). Toxicologic effects of gold nanoparticles in vivo by different administration routes. Int. J. Nanomed., 5: 771-781.

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