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Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 52 issue 4 (april 2018) : 538-542,   Doi: 10.18805/ijar.v0iOF.9131

The effect of fucoidan on the changes of some biochemical parameters and protein electrophoresis in hepatotoxicity induced by carbontetrachloride in rats

N
Nesrullah Aysin
H
Handan Mert
N
Nihat Mert
K
Kivanc Irak
1Department of Biochemistry, Faculty of Veterinary Medicine, Yuzuncu Yil University, Van, Turkey.
Cite article:- Aysin Nesrullah, Mert Handan, Mert Nihat, Irak Kivanc (2017). The effect of fucoidan on the changes of some biochemical parameters and protein electrophoresis in hepatotoxicity induced by carbontetrachloride in rats. Indian Journal of Animal Research. 52(4): 538-542. doi: 10.18805/ijar.v0iOF.9131.

ABSTRACT

Fucoidan is a sulfate polysaccharide extracted from brown algae. Fucoidan has various pharmacological properties, such as anti-tumor, anti-mutagenic, anti-inflammatory, antiviral, antioxidant, anti-fibrogenic activity and anti-complementary activities Objective: This study was aimed to explore the effect of fucoidan on biochemical parameters (ALT, AST, GGT, total protein, albumin, globulin) and protein fractions in hepatotoxicity induced by CCl4 in rats. Materials and Methods: The rats used in the study were randomly divided into four groups of 8 rats each: Control group, fucoidan group, fucoidan+CCl4 group and CCl4 group. After 24 hours from the process of an eight-day experiment, blood samples were taken. The analysis of ALT, AST, GGT activities and total protein, albumin, globulin levels were done by an autoanalyser and serum protein fractions (albumin, á1- globulin, á2-globulin â- globulin, ã- globulin and A/G ratio) were electrophoretically determined. Results: In the group of fucoidan+CCl4, it was determined that the levels of AST (p<0.001), GGT (p<0.001), total protein (p<0.01), globulin (p<0.01),  â-globulin % (p<0.01), ã-globulin  % (p<0.001) statistically decreased compared to CCl4 group. Conclusion: It can be said that fucoidan has the property of hepatoprotectant by looking to some biochemical parameters and changes in protein fractions that examined in hepatotoxicity induced by CCl4.

REFERENCES

  1. Ayaz, N.O., Ramadan, K.S., Farid Hoda E.A. and Alnahdi H.S.(2017). Protective role and antioxidant activity of arabic gum against trichloroacetate-induced toxicity in liver of male rats. Indian J. Anim. Res. 51(2):303-309.
  2. Boll, M., Weber, L.W., Becker, E. and Stampfl, A. (2001). Mechanism of carbon tetrachloride-induced hepatotoxicity. Hepatocellular damage by reactive carbon tetrachloride metabolites. Z Naturforsch C. 56: 649-659.
  3. Cao, G., Li, Q., Chen, X., Cai, H. and Tu, S. (2014). Hepatoprotective effect of superfine particles of herbal medicine against CCl4-    induced acute liver damage in rats. Biomed Res Int. doi: 10.1155/2014/934732. 
  4. El Shahat, A.N., El-Shennawy1, H.M. and Abd el-Megid M.H.M. (2017). Studying the protective effect of gamma-irradiated basil (Ocimum basilicum L.) against methotrexate-induced liver and renal toxicity in rats. Indian J. Anim. Res. 51(1):135-140.
  5. Feldman, S.C., Reynaldi, S., Stortz, C.A. and Cerezo, A.S. Damont, E.B. (1999). Antiviral properties of fucoidan fractions from Leathesia difformis. Phytomedicine. 6: 335-340.
  6. Hayashi, S., Itoh, A., Isoda, K., Kondoh, M. and Kawase, M., et al. (2008). Fucoidan partly prevents CCl4-induced liver fibrosis. European Journal of Pharmacology. 580: 380-384.
  7. Hirayama, C., Fukuda, T. and Toda, T. (1970). Metabolism of [131] gamma globulins in patients with chronic liver disease. Clin Chim Acta. 27: 409-413.
  8. Hong, S.W., Lee, H.S., Jung, K.H., Lee, H. and Hong, S.S. (2012). Protective effect of fucoidan against acetominophen induced liver injury. Arch Pharm Res. 35:1099-1105.
  9. Kala, S.M.J., Tresina, P.S. and Mohan, V.R. (2013). Hepatoprotective effect of Eugenia singampattiana Bedd leaf extract on carbon tetrachloride induced jaundice. International Journal of Pharmaceutical Sciences Review and Research. 21:41-45.
  10. Kang ,K.S., Kim, I.D., Kwon, R.H., Lee, J.Y. and Kang, J.S., et al. (2008). The effects of fucoidan extracts on CCl4-induced liver injury. Arch Pharm Res. 31: 622-627.
  11. Kober, H., Tatsch E., Torbitz, V.D., Cargnin, L.P. and Sangoi, M.B., et al. (2015). Genoprotective and hepatoprotective effects of Guarana (Paullinia cupana Mart. var. sorbilis) on CCl4-inducedliver damage in rats. Drug Chem Toxicol. 20:1-5. 
  12. Kurahori, T., Koda, T., Ichikawa, M. and Watanabe, Y. (1979). Phagocytic activity of reticulo endothelial system of patients with chronic liver diseases. Liver. 20: 249-260. 
  13. Kuriakose, G.C. and Kurup, G.M. (2008). Antioxidant activity of Aulosira fertilisima on CCl4 induced hepatotoxicity in rats. Indian J Exp Biol. 46:52-59.
  14. Li, B., Lu, F., Wei, X. and Zhao, R. (2008). Fucoidan: structure and bioactivity. Molecules. 13:1671-1695.
  15. Mohan, N.H., Maurya, S.K., Gangwar, A.K . and Srivastava, R.S. (2005). Kinetics of alkaline phosphatase isolated from rat liver, kidney and certain brain regions. Indian J. Anim. Res. 39 (2): 123-126.
  16. Molander, D.W., Wroblewski, F. and La Due, J.S. (1955). Transaminase compared with cholinesterase and alkaline phosphatase an index of hepatocellular integrity. Clinical Research Proceedings. 3: 20-24. 
  17. Murali, A., Ashok, P. and Madhavan, V. (2012). Antioxidant and hepatoprotective effects of the roots of Hemidesmus indicus (L.) R. Br var. pubescens (W.& A.) Hk f, Asian J Trad Med. 8: 36-46.
  18. Muriel P. (1998). Nitric oxide protection of rat liver from lipid peroxidation, collagen accumulation, and liver damageinduced by carbon tetrachloride. Biochemical Pharm. 56: 773-779.
  19. Okazaki, M., Furuya, E., Kasahara, T. and Sakamoto, K. (1985). Function of reticuloendothelial system on CCl4 induced liver injury in mice. Jpn Pharmacol. 39: 503-514.
  20. Ozer, J., Ratner, M., Shaw, M., Bailey, W. and Schomaker, S. (2008). The current state of serum biomarkers of hepatotoxicity. Toxicology. 245:194-205.
  21. Patel, G. (2010). Protective effect of nerium indicum on CCl4 induced hepatotoxicity in rats. International Journal of Biomedical Research. 1: 147-152. 
  22. Prakash, T., Fadadu, S.D., Sharma, U.R., Surendra V. and Goli, D., et al.. (2008). Hepatoprotective activity of leaves of Rhododendron arboreum in CCl4 induced hepatotoxicity in rats. Journal of Medicinal Plants Research. 2: 315-320.
  23. Rao, G.M.M., Rao, V.C., Pushpangadan, P. and Shirwaikar, A. (2006). Hepatoprotective effects of rubiadin, a major constituent of Rubia cordifolia Linn. Journal of Ethnopharmacology. 103: 484-490.
  24. Rocha de Souza, M.C., Marques, C.T., Guerra Dore, C.M., Ferreira da Silva, F.R. and Oliveira Rocha, H.A., et al. (2007). Antioxidant activities of sulfated polysaccharides from brown and red seaweeds. J Appl Phycol. 19:153-160.
  25. Shallan, M.A., El-Baz, G.D., Ali Hanaa, F.M. and Helmy, Y.S. (2008). Antioxicant effects against CCl4-induced liver damage of ripe fruits ethanol extract of Black Nightshades. Journal of biological and Chemical Environmental Science. 4:181-210.
  26. Shi, H., Liu, X., Tang, G., Liu, H. and Zhang, Y., et al. (2014). Ethanol extract of Portulaca Oleracea L. reduced the carbon tetrachloride induced liver injury in mice involving enhancement of NF-êB activity. Am J Transl Res. 6: 746-755.
  27. Veena, C.K., Josephine, A., Preetha, S.P. and Varalakshmi, P. (2007). Beneficial role of sulfated polysaccharides from edible seaweed Fucus vesiculosus in experimental hyperoxaluria. Food Chemistry. 100: 1552-1559.
  28. Wang, J,, Zhang, Q,, Jin, W,, Niu, X, and Zhang, H. (2011). Effects and mechanism of low molecular weight fucoidan in mitigating the peroxidative and renal damage induced by adenine. Carbohydrate Polymers. 84:417-423.
  29. Yang, Y.S., Ahn, T.H., Lee, J.C., Moon, C.J. and Kim, S.H., et al. (2008). Protective effects of Pycnogenol on carbon tetra chloride induced hepatotoxicity in Sprague-Dawley rats. Food Chem Toxicol. 46:380-387.
  30. Zhang, Q.B., Yu, P.Z., Zhou, G.F., Li, M.Z.E. and Xu, Z.H. (2003). Studies on antioxidant activities of fucoidan from Laminaria japonica. Chin Trad Herbal Drugs. 34: 824-826. 
  31. Zimmerman, H.J. and Seeff, L.B. (1970). Enzymes in hepatic disease Diagnostic Enzymology, Goodley EL (Ed.), Lea and Febiger, Philadelphia 1-38
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Indian Journal of Animal Research
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