Chief EditorK.M.L. Pathak
Print ISSN 0367-6722
Online ISSN 0976-0555
NAAS Rating 6.43
Impact Factor 0.5 (2023)
Immune profiles of T lymphocyte subsets in adipose tissue of obese mouse induced by high-fat diet
First Online 06-09-2017|
In this study, we analyzed high-fat diet (HFD)-induced time-course changes in proportions of T lymphocyte subsets in adipose tissue.Mice were fed with normal-fat diet (NFD) or HFD for 20 weeks. An autoanalyzer was used to assay plasma concentrations of glucose, cholesterol, triglycerides, low-density lipoprotein (LDL), and high-density lipoprotein (HDL). Stromal vascular cells were isolated from epididymal adipose tissues and labeled with antibodies for cluster of differentiation (CD) 3, CD4, CD8, interferon -g, interleukin (IL)-4, and IL-17 for fluorescence-activated cell sorter. We discovered that weight of epididymal fat pads and perirenal fat in HFD group were higher than that in NFD group. Significant changes in glucose, cholesterol, triglyceride, LDL, and HDL content were detected in sera of mice fed with HFD compared with those provided with NFD. Proportions of CD3+, CD4+, and CD8+ T cells increased significantly in adipose tissues of HFD mice compared with those of NFD mice. Proportions of T helper (Th)1 and Th17 sublineages also increased significantly in HFD group. Long-time HFD-induced obesity can increase proportions of CD3+ T, CD4+ T, and CD8+ T cells in epididymal fat pads, disrupt balance of CD4+ T lymphocyte subsets, and induce progressive Th1 and Th17 biases.
- Aarti S., Archana S. and Nidhi S. (2008). Effect of aerobics on blood pressure, body mass index and lipid profile of normal and obese subjects. J. Dairying, Foods & H.S., 27:134-137.
- Andersen, C. J., Murphy, K. E. and Fernandez, M. L. (2016). Impact of obesity and metabolic syndrome on immunity. Adv. Nutr. 7: 66-75.
- Anderson, E. K., Gutierrez, D. A., Kennedy, A. and Hasty, A. H. (2013). Weight cycling increases T-cell accumulation in adipose tissue and impairs systemic glucose tolerance. Diabetes, 62: 3180-3188.
- Apostolopoulos, V., de Courten, M. P., Stojanovska, L., Blatch, G. L., Tangalakis, K. and de Courten, B. (2016). The complex immunological and inflammatory network of adipose tissue in obesity. Mol. Nutr. Food Res. 60: 43-57.
- Cildir, G., Akincilar, S. C. and Tergaonkar, V. (2013). Chronic adipose tissue inflammation: all immune cells on the stage. Trends Mol. Med. 19: 487-500.
- Duffaut, C., Zakaroff-Girard, A., Bourlier, V., Decaunes, P., Maumus, M., Chiotasso, P., Sengenes, C., Lafontan, M., Galitzky, J. and Bouloumie, A. (2009). Interplay between human adipocytes and T lymphocytes in obesity: CCL20 as an adipochemokine and T lymphocytes as lipogenic modulators. Arterioscler Thromb. Vasc. Biol. 29: 1608-1614.
- Emanuela F, G. M., Marco de R, Maria Paola L, Giorgio F. and Marco B. (2012). Inflammation as a link between obesity and metabolic syndrome. J. Nutr. Metab. 2012: 476380.
- Exley, M. A., Hand, L., O’Shea, D. and Lynch, L. (2014). Interplay between the immune system and adipose tissue in obesity. J. Endocrinol. 223: R41-R48.
- Frederick, C. B., Snellman, K. and Putnam, R. D. (2014). Increasing socioeconomic disparities in adolescent obesity. Proc. Natl. Acad. Sci. U. S. A. 111: 1338-1342.
- Han, J. M. and Levings, M. K. (2013). Immune regulation in obesity-associated adipose inflammation. J. Immunol. 191: 527-532.
- Kaminski, D. A. and Randall, T. D. (2010). Adaptive immunity and adipose tissue biology. Trends Immunol. 31: 384-390.
- Makki, K., Froguel, P. and Wolowczuk, I. (2013). Adipose tissue in obesity-related inflammation and insulin resistance: cells, cytokines, and chemokines. ISRN Inflamm. 2013: 139239.
- Mathis, D. (2013). Immunological goings-on in visceral adipose tissue. Cell Metab. 17: 851-859.
- Morris, D. L., Cho, K. W., Delproposto, J. L., Oatmen, K. E., Geletka, L. M., Martinez-Santibanez, G., Singer, K. and Lumeng, C. N. (2013). Adipose tissue macrophages function as antigen-presenting cells and regulate adipose tissue CD4+ T cells in mice. Diabetes, 62: 2762-2772.
- Nishimura, S., Manabe, I., Nagasaki, M., Eto, K., Yamashita, H., Ohsugi, M., Otsu, M., Hara, K., Ueki, K., Sugiura, S., Yoshimura, K., Kadowaki, T. and Nagai, R. (2009). CD8+ effector T cells contribute to macrophage recruitment and adipose tissue inflammation in obesity. Nat. Med. 15: 914-920.
- Rocha, V. Z., Folco, E. J., Sukhova, G., Shimizu, K., Gotsman, I., Vernon, A. H. and Libby, P. (2008). Interferon-gamma, a Th1 cytokine, regulates fat inflammation: a role for adaptive immunity in obesity. Circ. Res. 103: 467-476.
- Tateya, S., Kim, F. and Tamori, Y. (2013). Recent advances in obesity-induced inflammation and insulin resistance. Front. Endocrinol (Lausanne). 4: 93.
- Watanabe, Y., Nagai, Y. and Takatsu, K. (2013). Activation and regulation of the pattern recognition receptors in obesity-induced adipose tissue inflammation and insulin resistance. Nutrients 5: 3757-3778.
- Winer, D. A., Winer, S., Chng, M. H., Shen, L. and Engleman, E. G. (2013). B Lymphocytes in obesity-related adipose tissue inflammation and insulin resistance. Cell. Mol. Life Sci. 71: 11.
- Winer, S. and Winer, D. A. (2012). The adaptive immune system as a fundamental regulator of adipose tissue inflammation and insulin resistance. Immunol. Cell Biol. 90: 755-762.
- Yu, L., Yan, K., Liu, P., Li, N., Liu, Z., Zhu, W., Chen, Y. and Han, D. (2014). Pattern recognition receptor-initiated innate antiviral response in mouse adipose cells. Immunol. Cell Biol. 92: 105-115.
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