Banner
Current IssueEditorial BoardOnline First ArticlesArchive
Current IssueEditorial BoardOnline First ArticlesArchive

Research Article

volume 53 issue 11 (november 2019) : 1522-1525,   Doi: 10.18805/ijar.v0iOF.7251

Antimicrobial resistance of Vibrio (Listonella) anguillarum isolated from rainbow trouts (Oncorhynchus mykiss)

U
U. Parin
G
G. Erbas
S
S. Savasan
H
H.T. Yuksel
S
S. Gurpinar
S
S. Kirkan
1Department of Microbiology, Adnan Menderes University, Faculty of Veterinary Medicine, 09016, Isikli Aydin, Turkey.
Cite article:- Parin U., Erbas G., Savasan S., Yuksel H.T., Gurpinar S., Kirkan S. (2017). Antimicrobial resistance of Vibrio (Listonella) anguillarum isolated from rainbow trouts (Oncorhynchus mykiss). Indian Journal of Animal Research. 53(11): 1522-1525. doi: 10.18805/ijar.v0iOF.7251.

ABSTRACT

Vibrio (also known as Listonella) species are widely distributed in aquatic environments and are often mentioned as the aquatic equivalent of aeromonads. The aim of this study is to investigate the exposure of rainbow trouts with V. anguillarum, and determination of antimicrobial resistance of the isolated strains. A total of 100 rainbow trout (Oncorhynchus mykiss) samples were collected from various commercial fish hatcheries in the Aegean Region of Turkey in June 2013. Twelve                V. anguillarum were isolated from 100 rainbow trout (Oncorhynchus mykiss) samples. V. anguillarum isolates were resistant to cloxacillin, ampicillin, sulfamethoxazol-trimethoprime and erythromycin in the ratio of 100 %. High levels of resistance are considered to be the result of random antibiotic therapies in aquaculture, hence the results of this study indicated that identification of etiological agent and the exact chemotherapy must be applied in order to prevent residual water contamination and resistant strains of V. anguillarum.

REFERENCES

  1. Akayli, T. (2001). Determination of pathogenic characteristics of Vibrio strains in gilthead seabream (Sparus aurata L.) Istanbul Unv, Institute of Science, PhDThesis, Istanbul.
  2. Akinbowale, O.L., Peng, H. and Barton, M.D.(2006). Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. J. App. Microbiol. 100:1103-1113.
  3. Alcaide, E., Blasco, M.D. and Esteve, C. (2004). Occurrence of drug-resistance in twoEuropean eel farms. Appl. Environ. Microbiol. 71:3348-3350.
  4. Alderman, D.J. and Smith, P. (2001). Development of draft protocols of standard reference methods for antimicrobial agent susceptibility testing of bacteria associated with fish diseases. Aquacult. 196:211–243.
  5. Austin, B. andAustin, A. (2007). Bacterial fish pathogens. In: Diseasesof farmed and wild fish,Springer-Praxis Publishing, Chichester, U.K. 4: 411.
  6. Bruno, D.W., Alderman, D.J. and Schlotfeldt, H.J. (1997). What should I do? Apractical guide for the marine fish farmer. ISBN 0-    9526242-2-2.
  7. C.L.S.I. (2011). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals; Approved Standard. 3rd ed. Document M31-A3, vol 28 No. 8. Villanova. Pa. National Committee For Clinical Laboratory Standards, Clinical and Laboratory Standards Institute.
  8. Colquhoun, D.J., Aarflot, L. and Melvold, C.F. (2007). gyrA and parC mutations and associated quinolone resistance inVibrio anguillarum serotype O2b strains isolated from farmed atlantic cod (Gadus morhua) in Norway.Antimicrobial Agents Chemother. 51:2597–    2599.
  9. Demircan, D. and Candan, A. (2006). Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with Vibriosis in marine fish in Turkey. Turk. J. Vet. Anim. Sci. 30:305-310.
  10. Farmer, J.J. and Janda, J.M. (2005). Family Vibrionaceae. In: Bergey’s manual of systematic bacteriology by Brenner, B.J., Krieg, N.R. and StaleyJ.T.(ed),Springer, New York, U.S.A. 1: 491-494.
  11. Guardabassi, L., Dalsgaard, A., Raffatellu, M. and Olesen, J.E. (2000). Increase in the prevalence of oxolinic acid resistant Acinetobacter spp. observed in a stream receiving the effluent from a freshwater trout farm following the treatment with oxolinic acid-    medicated feed. Aquacult. 188:205-218.
  12. Korun, J. and Timur, G. (2008). Marine vibriosis associated with diseased sea bass (Dicentrachus labrax) in Turkey. J. Fish. Sci. 2:66-76.
  13. Lamas, J., Santos, Y., Bruno, D., Toranzo, A.E. and Anadon, R. (1994). A comparison of pathological changes caused by Vibrio anguillarum and its extracellular products in rainbow trout (Oncorhynchus mykiss). Fish Pathol. 29:79-89.
  14. Lunder, T., Sørum, H., Holstad, G., Steigerwalt, A.G., Mowinckel, P. and Brenner, D.J. (2000). Phenotypic and genotypic characterization of Vibrio viscosus sp. nov. and Vibrio wodanis sp. nov. isolated from Atlantic salmon (Salmo salar) with ‘winter ulcer. Int.J. Syst.Evol. Microbiol. 50:427–450.
  15. McPhearson, R.M., DePaola, A., Zywno, S.R., Motes, M.L. andGuarino, A.M. (1991). Antibiotic resistance in Gram negative bacteria from cultured catfish and aquacultureponds. Aquacult. 99:203-211.
  16. Noga, E.J. (2010). Diagnosis and treatment. In: Fish Disease, Blackwell Publishing, Singapore.1: 286.
  17. Oliver, J.D. and Japer,J.B. (1997).Vibrio species. In: Food Microbiology–Fundamentals and Frontiers, ASM Press, Washington, U.S.A. 1: 264.
  18. Reed, P.A. and Francis-Floyd, R. (1996). Vibrio infections of fish. Fisheries and Aquatic Sciences Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, FA31.
  19. Roberts,R.J. (2001). Fish Pathology. Saunders W.B., London, U.K. 1: 137.
  20. Schmidt, A.S., Bruun, M.S., Dalsgaard, I., Pedersone, K. and Larsen, J.L. (2000). Occurrence of antimicrobial resistance in fish-pathogenic and environmental bacteria associated with four Danish rainbow trout farms. App. Environ. Microbiol. 66:4908-4915.
  21. Smith, K.F., Schmidt, V., Rosen, G.E., Amaral-Zettler, L. (2012). Microbial diversity and potential pathogens in ornamental fish aquarium water. PLOS ONE Open Access. 7: 1-11.
  22. Sutherland, J. and Varnam, A. (2000). Enterotoxin-producing Staphylococcus, Shigella, Yersinia, Vibrio, Aeromonas and Plesiomonas. In: Foodborne pathogens, hazards, risk analysis and control,Woodhead Publishing Limited, Cambridge, U.K. 1: 415.
  23. Tanrikul, T., Cagirgan, H., Toksen,H.E. (2004). Identification of isolated vibrio sp. from sea bass (Dicentrarchus labrax L., 1758) using API 20E System. Ege Univ. J. Aquacult. 21: 243–247.
  24. Tanrikul,T. (2007). Vibriosis as an epizootic disease of rainbow trout (Onchorynchus mykiss) in Turkey. Pak.J. Biol. Sci.10:1733-1737.
  25. Timur, G., Korun, J. (2004). First outbreak of vibriosis in farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Ist. Univ. J. Fish. Aquat.Sci.18:1-9.
  26. Vaseeharan, B., Ramasamy, P., Murugan, T. and Chen, J.C. (2005). In vitro susceptibility of antibiotics against Vibrio spp. and Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds. Int. J. Antimicrob.Agent. 26:285-291.
  27. Woo, P.T.K. and Bruno, D.W. (1999).Fish Diseases and Disorders, CABI Publishing, U.K. 1: 194.
Published In
Indian Journal of Animal Research
Article Metrics
Metrics Icon
0
Views
0
Citations
Reviewed By
Share Article
Download Article
In this Article
    Contact us
    Follow us

    Research Article

    volume 53 issue 11 (november 2019) : 1522-1525,   Doi: 10.18805/ijar.v0iOF.7251

    Antimicrobial resistance of Vibrio (Listonella) anguillarum isolated from rainbow trouts (Oncorhynchus mykiss)

    U
    U. Parin
    G
    G. Erbas
    S
    S. Savasan
    H
    H.T. Yuksel
    S
    S. Gurpinar
    S
    S. Kirkan
    1Department of Microbiology, Adnan Menderes University, Faculty of Veterinary Medicine, 09016, Isikli Aydin, Turkey.
    Cite article:- Parin U., Erbas G., Savasan S., Yuksel H.T., Gurpinar S., Kirkan S. (2017). Antimicrobial resistance of Vibrio (Listonella) anguillarum isolated from rainbow trouts (Oncorhynchus mykiss). Indian Journal of Animal Research. 53(11): 1522-1525. doi: 10.18805/ijar.v0iOF.7251.

    ABSTRACT

    Vibrio (also known as Listonella) species are widely distributed in aquatic environments and are often mentioned as the aquatic equivalent of aeromonads. The aim of this study is to investigate the exposure of rainbow trouts with V. anguillarum, and determination of antimicrobial resistance of the isolated strains. A total of 100 rainbow trout (Oncorhynchus mykiss) samples were collected from various commercial fish hatcheries in the Aegean Region of Turkey in June 2013. Twelve                V. anguillarum were isolated from 100 rainbow trout (Oncorhynchus mykiss) samples. V. anguillarum isolates were resistant to cloxacillin, ampicillin, sulfamethoxazol-trimethoprime and erythromycin in the ratio of 100 %. High levels of resistance are considered to be the result of random antibiotic therapies in aquaculture, hence the results of this study indicated that identification of etiological agent and the exact chemotherapy must be applied in order to prevent residual water contamination and resistant strains of V. anguillarum.

    REFERENCES

    1. Akayli, T. (2001). Determination of pathogenic characteristics of Vibrio strains in gilthead seabream (Sparus aurata L.) Istanbul Unv, Institute of Science, PhDThesis, Istanbul.
    2. Akinbowale, O.L., Peng, H. and Barton, M.D.(2006). Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia. J. App. Microbiol. 100:1103-1113.
    3. Alcaide, E., Blasco, M.D. and Esteve, C. (2004). Occurrence of drug-resistance in twoEuropean eel farms. Appl. Environ. Microbiol. 71:3348-3350.
    4. Alderman, D.J. and Smith, P. (2001). Development of draft protocols of standard reference methods for antimicrobial agent susceptibility testing of bacteria associated with fish diseases. Aquacult. 196:211–243.
    5. Austin, B. andAustin, A. (2007). Bacterial fish pathogens. In: Diseasesof farmed and wild fish,Springer-Praxis Publishing, Chichester, U.K. 4: 411.
    6. Bruno, D.W., Alderman, D.J. and Schlotfeldt, H.J. (1997). What should I do? Apractical guide for the marine fish farmer. ISBN 0-    9526242-2-2.
    7. C.L.S.I. (2011). Performance Standards for Antimicrobial Disk and Dilution Susceptibility Tests for Bacteria Isolated From Animals; Approved Standard. 3rd ed. Document M31-A3, vol 28 No. 8. Villanova. Pa. National Committee For Clinical Laboratory Standards, Clinical and Laboratory Standards Institute.
    8. Colquhoun, D.J., Aarflot, L. and Melvold, C.F. (2007). gyrA and parC mutations and associated quinolone resistance inVibrio anguillarum serotype O2b strains isolated from farmed atlantic cod (Gadus morhua) in Norway.Antimicrobial Agents Chemother. 51:2597–    2599.
    9. Demircan, D. and Candan, A. (2006). Identification of Vibrio anguillarum by PCR (rpoN Gene) associated with Vibriosis in marine fish in Turkey. Turk. J. Vet. Anim. Sci. 30:305-310.
    10. Farmer, J.J. and Janda, J.M. (2005). Family Vibrionaceae. In: Bergey’s manual of systematic bacteriology by Brenner, B.J., Krieg, N.R. and StaleyJ.T.(ed),Springer, New York, U.S.A. 1: 491-494.
    11. Guardabassi, L., Dalsgaard, A., Raffatellu, M. and Olesen, J.E. (2000). Increase in the prevalence of oxolinic acid resistant Acinetobacter spp. observed in a stream receiving the effluent from a freshwater trout farm following the treatment with oxolinic acid-    medicated feed. Aquacult. 188:205-218.
    12. Korun, J. and Timur, G. (2008). Marine vibriosis associated with diseased sea bass (Dicentrachus labrax) in Turkey. J. Fish. Sci. 2:66-76.
    13. Lamas, J., Santos, Y., Bruno, D., Toranzo, A.E. and Anadon, R. (1994). A comparison of pathological changes caused by Vibrio anguillarum and its extracellular products in rainbow trout (Oncorhynchus mykiss). Fish Pathol. 29:79-89.
    14. Lunder, T., Sørum, H., Holstad, G., Steigerwalt, A.G., Mowinckel, P. and Brenner, D.J. (2000). Phenotypic and genotypic characterization of Vibrio viscosus sp. nov. and Vibrio wodanis sp. nov. isolated from Atlantic salmon (Salmo salar) with ‘winter ulcer. Int.J. Syst.Evol. Microbiol. 50:427–450.
    15. McPhearson, R.M., DePaola, A., Zywno, S.R., Motes, M.L. andGuarino, A.M. (1991). Antibiotic resistance in Gram negative bacteria from cultured catfish and aquacultureponds. Aquacult. 99:203-211.
    16. Noga, E.J. (2010). Diagnosis and treatment. In: Fish Disease, Blackwell Publishing, Singapore.1: 286.
    17. Oliver, J.D. and Japer,J.B. (1997).Vibrio species. In: Food Microbiology–Fundamentals and Frontiers, ASM Press, Washington, U.S.A. 1: 264.
    18. Reed, P.A. and Francis-Floyd, R. (1996). Vibrio infections of fish. Fisheries and Aquatic Sciences Department, Florida Cooperative Extension Service, Institute of Food and Agricultural Sciences, University of Florida, FA31.
    19. Roberts,R.J. (2001). Fish Pathology. Saunders W.B., London, U.K. 1: 137.
    20. Schmidt, A.S., Bruun, M.S., Dalsgaard, I., Pedersone, K. and Larsen, J.L. (2000). Occurrence of antimicrobial resistance in fish-pathogenic and environmental bacteria associated with four Danish rainbow trout farms. App. Environ. Microbiol. 66:4908-4915.
    21. Smith, K.F., Schmidt, V., Rosen, G.E., Amaral-Zettler, L. (2012). Microbial diversity and potential pathogens in ornamental fish aquarium water. PLOS ONE Open Access. 7: 1-11.
    22. Sutherland, J. and Varnam, A. (2000). Enterotoxin-producing Staphylococcus, Shigella, Yersinia, Vibrio, Aeromonas and Plesiomonas. In: Foodborne pathogens, hazards, risk analysis and control,Woodhead Publishing Limited, Cambridge, U.K. 1: 415.
    23. Tanrikul, T., Cagirgan, H., Toksen,H.E. (2004). Identification of isolated vibrio sp. from sea bass (Dicentrarchus labrax L., 1758) using API 20E System. Ege Univ. J. Aquacult. 21: 243–247.
    24. Tanrikul,T. (2007). Vibriosis as an epizootic disease of rainbow trout (Onchorynchus mykiss) in Turkey. Pak.J. Biol. Sci.10:1733-1737.
    25. Timur, G., Korun, J. (2004). First outbreak of vibriosis in farmed rainbow trout (Oncorhynchus mykiss) in Turkey. Ist. Univ. J. Fish. Aquat.Sci.18:1-9.
    26. Vaseeharan, B., Ramasamy, P., Murugan, T. and Chen, J.C. (2005). In vitro susceptibility of antibiotics against Vibrio spp. and Aeromonas spp. isolated from Penaeus monodon hatcheries and ponds. Int. J. Antimicrob.Agent. 26:285-291.
    27. Woo, P.T.K. and Bruno, D.W. (1999).Fish Diseases and Disorders, CABI Publishing, U.K. 1: 194.
    In this Article
      Contact us
      Follow us
      Published In
      Indian Journal of Animal Research

      Editorial Board

      View all (0)