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Research Article

volume 52 issue 5 (may 2018) : 718-723,   Doi: 10.18805/ijar.v0iOF.9128

Effect of dietary aflatoxins (AFB1) on hematological and biochemical indices of male buffaloes

O
Omer Naseer
J
Jawaria Ali Khan
M
Muhammad Sarwar Khan
M
Muhammad Ovais Omer
J
Junaid Naseer
M
Muhmmad Luqman Sohail
M
Muhmmad Usman Saleem
W
Waqas Ahmad
A
Abdullah Saghir Ahmad
1Department of Clinical Medicine and Surgery, University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan.
Cite article:- Naseer Omer, Khan Ali Jawaria, Khan Sarwar Muhammad, Omer Ovais Muhammad, Naseer Junaid, Sohail Luqman Muhmmad, Saleem Usman Muhmmad, Ahmad Waqas, Ahmad Saghir Abdullah (2017). Effect of dietary aflatoxins (AFB1) on hematological and biochemical indices of male buffaloes. Indian Journal of Animal Research. 52(5): 718-723. doi: 10.18805/ijar.v0iOF.9128.

ABSTRACT

The objective of the study was to determine the effect of dietary aflatoxin B1 (AFB1) in growing male buffaloes in terms of feed intake, hematological and serum biochemical parameters. Thirty six bovine growing male buffaloes between 6 months to 12 months of age were divided into four groups to receive diet having no AFB1, 0.6mg/kg, 0.8mg/kg and 1.0mg/kg AFB1 of feed per animal per day for 28 days. The results indicate that the average daily feed intake of AFB1-treated growing male buffaloes significantly declined (P < 0.05) compared with the control. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatinine were significantly increased (P < 0.05) in response to AFB1, However change in blood urea nitrogen (BUN) levels was non-significant values of all hematological parameters decreased significantly (P < 0.05) in1.0mg/kg AFB1 group as compared to other groups after day 14 onward.

REFERENCES

  1. Abbes, S., Salah-Abbes, J.B., Abdel-Wahhab, M.A. and Ouslati, R. (2010) Immunotoxicological and biochemical effects of aflatoxins in rats prevented by Tunisian montmorillonite with reference to HSCAS. Immunophram. Immunot. 32:514-522.
  2. Abdel-Rahman, A. and OKLE, O. (2014) Aflatoxins Aggravate the Incidence of Salmonellosis Outbreak in Fattening Calves: A Case Study. Int. J. Biol. Biomol. Agri. Food. Biotechnol. Eng. 8 (2):109-111.
  3. Afzal, M., Anwar, M., Mirza, M.A. and Andrabi, S.M.H. (2009) Comparison of Growth Rate of Male Buffalo Calves under Open Grazing and Stall Feeding System. Pak. J. Nutr. 8 (2): 187-188.
  4. Akhtar, R., Sardar, M., Saima, N., Saleem, G., Imran, S. and Aslam, A. (2014) Responses of Nili- Ravi buffalo to aflatoxin B1 with and without toxin adsorbents. J. Anim. Feed Sci. 23:317-323.
  5. Chohan, K.A., Awan, F., Ali, M.M., Iqbal, U. and Ijaz, M. (2016) Assessment of Aflatoxin in Dairy Concentrate Feeds, Total Mixed Rations, Silage and Various Feed Ingredients in Pakistan. Pak. J. Zool. 48(1): 277-280.
  6. Donmez, N., Donmez, H.H., Keskin, E. and Kýsadere, I. (2012) Effects of Aflatoxin on Some Haematological Parameters and Protective Effectiveness of Esterified Glucomannan in Merino Rams. Scientific World J. 2012: 4. doi:10.1100/2012/342468.
  7. Eraslan, G., Akdogan, M., Liman, B.C., Kanbur, M., Delibas, N. (2006) Effects of dietary aflatoxin and hydrated sodium calcium aluminosilicate on triiodothyronine, thyroxin, thyrotrophin and testosterone levels in quails. Turk. J Vet. Anim. Sci. 30: 41–45.
  8. Fapohunda, S.O., Ezekiel, C.N., Alabi, O.A., Omole, A. and Chioma, S.O. (2008) Aflatoxin- mediated Sperm and Blood Cell Abnormalities in Mice Fed with Contaminated Corn. Mycobiology. 36(4): 255-259.
  9. Fink-Gremmels, J. (2008) Mycotoxins in cattle feeds and carry-over to dairy milk: A review. Food Addit Contam25: 172-180.
  10. Gajecka, M., Jakimiuk, E., Polak, M., Otrocka-Domagala, I., Janowski, T., Zwierzchowski, W., Obremski, K., Zielonka, L., Apoznanski, J., Gajecki, M. (2004) Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system. Pol. J. Vet. Sci. 7: 59-66.
  11. Gowda, N.K.S., Ledoux, D.R., Rottinghaus, G.E., Bermudez, A.J., Chen, Y.C. (2009) Antioxidant efficacy of curcuminoids from turmeric (Curcuma longa L.) powder in broiler chickens fed diets containing aflatoxin B1. Br. J. Nutr. 102: 1629–1634. 
  12. Harriet, A.M. (2003) Is indoor mold contamination a threat to health? J. Environ. Health. 62(2): 0022-0892.
  13. Hassan, A.M., Abdel-Aziem, S.H., El-Nekeety, A.A., Abdel-Wahhab, M.A. (2015) Panax ginseng extract modulates oxidative stress, DNA fragmentation and up-regulate gene expression in rats sub chronically treated with aflatoxin B1 and fumonisin B1. Cytotechnol. 67:861–871.
  14. Jovaisiene, J., Bakutis, B., Baliukoniene, V., Gerulis, G. (2016) Fusarium and Aspergillus mycotoxins effects on dairy cow health, performance and the efficacy of Anti-Mycotoxin Additive. Pol. J. Vet. Sci. 19(1): 79–87.
  15. Lakkawar, A.W., Chattopadhyay, S.K., Johri, T.S. (2004) Experimental aflatoxin B1 toxicosis in young rabbits- A clinical and patho-    anatomical study. Slov. Vet. Res. 41: 73-81.
  16. Mohammed, A.M., Metwally, N.S. (2009) Antiaflatoxicogenic activities of some aqeous plant extracts against AFB1 induced Renal and Cardiac damage. J. Pharmacol. Toxicol.. 4(1): 1-16.
  17. Murugesan, G.R., Ledoux, D.R., Naehrer, K., Berthiller, F., Applegate, T.J., Grenier, B., Phillips, T.D. and Schatzmayr, G. (2015) Prevalence and effects of mycotoxins on poultry health and performance, and recent development in mycotoxin counteracting strategies. Poult. Sci. 94:1298–1315.
  18. Naseer, O., Khan, J.A., Khan, M.S., Omer, M.O., Chishti, G.A., Sohail, M.L., Saleem, M.U. (2016) Comparative efficacy of silymarin and choline chloride (liver tonics) in preventing the effects of aflatoxin B1 in bovine calves. Pol. J. Vet. Sci. 19 (3): 545–551.
  19. Pasha, T.N. (2008) Detoxification of aflatoxin by yeast sludge in the feed of dairy cattle and its impact on increased milk production. Int. J. Agro. Vet. Med. Sci. 2: 18-26.
  20. Paterson, R.R.M. (2007) Aflatoxins contamination in chili samples from Pakistan. Food Control. 18: 817–820.
  21. Pastorelli, H., Milegen, J.V., Lovatto, P. and Montagne, L. (2012) Meta-analysis of feed intake and growth responses of growing pigs after a sanitary challenge. Animal. 6:952–961.
  22. Pleadin, J., Vuli, A., Persi, N., Skrivanko, M., Capek, B., Cvetnic, Z. (2015) Annual and regional variations of aflatoxin B1 levels seen in grains and feed coming from Croatian dairy farms over a 5-year period. Food Cont. 47: 221-225.
  23. Samuel, N., Ezrib, Y., Faraha, R., Igore, V., Husseinc, A., Rubinshteina, O., Assy, N. (2009) Acute Aflatoxicosis Resulting in Fulminant Hepatic Failure and Rhabdomyolysis. Gastroenterol. Res. 2 (1): 48-50.
  24. Sharma, V., Sharma, C., Paliwal, R., Pracheta, S.S. and Sharma, S. (2011) Ameliorative Effects of Curcuma Longa and Curcumin on Aflatoxin B1 Induced Serological and Biochemical Changes In Kidney of Male Mice. Asian J. Biochem. Pharm. Res. 1 (2): 338-351. 
  25. Thrasher, J.D. (2012) Aflatoxicosis in animals. Aflatoxins and Health, www.alpha boostjuice.com/AFLATOXICOSIS_IN_ANIMALS.pdf.
  26. Tripathi, M.K., Mondal, D., Karim, S.A. (2008) Growth, haematology, blood constituents and immunological status of lambs fed graded levels of animal feed grade damaged wheat as substitute of maize. J. Anim. Physiol. Anim. Nutr. 92: 75-85.
  27. Wangikar, P.B., Dwivedi, P., Sinha, N., Sharma, A.K., Telang, A.G. (2005) Teratogenic effects in rabbits of simultaneous exposure to ochratoxin A and aflatoxin B1 with special reference to microscopic effects. Toxicol. 215: 37-47. 
  28. Whitlow, L.W. and Hagler, Jr. W.M. (2005) Mycotoxins in dairy cattle: occurrence, toxicity, prevention and treatment. Proc. Southwest Nutrition Conference 124- 138.
  29. Wu, F. .and Khlangwiset, P (2010) Health economic impacts and cost-effectiveness of aflatoxin reduction strategies in Africa: Case studies in biocontrol and postharvest interventions. Food Addit. Contam. 27: 496-509.
  30. Wu, F. and Tritscher, A. (2011) Aflatoxins a global public health problem: World Health Organization, available at: https://orecomm.net/    wp-content/uploads/2011/12/WHO-Aflatoxin-public-health-issue.pdf .
  31. Yunus, W.A., Sulyok, M. and Böhm, J. (2015) Mycotoxin Cocktail in the Samples of Oilseed Cake from Early Maturing Cotton Varieties Associated with Cattle Feeding Problems. Toxins. 7: 2188-2197.
  32. Yarru, L.P., Settivari, R.S., Antoniou, E., Ledoux, D.R. and Rottinghaus, G.E. (2009) Toxicological and gene expression analysis of the impact of aflatoxin B1 on hepatic function of male broiler chicks. Poult. Sci. 88:360–371. 
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Indian Journal of Animal Research
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    Research Article

    volume 52 issue 5 (may 2018) : 718-723,   Doi: 10.18805/ijar.v0iOF.9128

    Effect of dietary aflatoxins (AFB1) on hematological and biochemical indices of male buffaloes

    O
    Omer Naseer
    J
    Jawaria Ali Khan
    M
    Muhammad Sarwar Khan
    M
    Muhammad Ovais Omer
    J
    Junaid Naseer
    M
    Muhmmad Luqman Sohail
    M
    Muhmmad Usman Saleem
    W
    Waqas Ahmad
    A
    Abdullah Saghir Ahmad
    1Department of Clinical Medicine and Surgery, University College of Veterinary and Animal Sciences, The Islamia University of Bahawalpur, Pakistan.
    Cite article:- Naseer Omer, Khan Ali Jawaria, Khan Sarwar Muhammad, Omer Ovais Muhammad, Naseer Junaid, Sohail Luqman Muhmmad, Saleem Usman Muhmmad, Ahmad Waqas, Ahmad Saghir Abdullah (2017). Effect of dietary aflatoxins (AFB1) on hematological and biochemical indices of male buffaloes. Indian Journal of Animal Research. 52(5): 718-723. doi: 10.18805/ijar.v0iOF.9128.

    ABSTRACT

    The objective of the study was to determine the effect of dietary aflatoxin B1 (AFB1) in growing male buffaloes in terms of feed intake, hematological and serum biochemical parameters. Thirty six bovine growing male buffaloes between 6 months to 12 months of age were divided into four groups to receive diet having no AFB1, 0.6mg/kg, 0.8mg/kg and 1.0mg/kg AFB1 of feed per animal per day for 28 days. The results indicate that the average daily feed intake of AFB1-treated growing male buffaloes significantly declined (P < 0.05) compared with the control. Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and creatinine were significantly increased (P < 0.05) in response to AFB1, However change in blood urea nitrogen (BUN) levels was non-significant values of all hematological parameters decreased significantly (P < 0.05) in1.0mg/kg AFB1 group as compared to other groups after day 14 onward.

    REFERENCES

    1. Abbes, S., Salah-Abbes, J.B., Abdel-Wahhab, M.A. and Ouslati, R. (2010) Immunotoxicological and biochemical effects of aflatoxins in rats prevented by Tunisian montmorillonite with reference to HSCAS. Immunophram. Immunot. 32:514-522.
    2. Abdel-Rahman, A. and OKLE, O. (2014) Aflatoxins Aggravate the Incidence of Salmonellosis Outbreak in Fattening Calves: A Case Study. Int. J. Biol. Biomol. Agri. Food. Biotechnol. Eng. 8 (2):109-111.
    3. Afzal, M., Anwar, M., Mirza, M.A. and Andrabi, S.M.H. (2009) Comparison of Growth Rate of Male Buffalo Calves under Open Grazing and Stall Feeding System. Pak. J. Nutr. 8 (2): 187-188.
    4. Akhtar, R., Sardar, M., Saima, N., Saleem, G., Imran, S. and Aslam, A. (2014) Responses of Nili- Ravi buffalo to aflatoxin B1 with and without toxin adsorbents. J. Anim. Feed Sci. 23:317-323.
    5. Chohan, K.A., Awan, F., Ali, M.M., Iqbal, U. and Ijaz, M. (2016) Assessment of Aflatoxin in Dairy Concentrate Feeds, Total Mixed Rations, Silage and Various Feed Ingredients in Pakistan. Pak. J. Zool. 48(1): 277-280.
    6. Donmez, N., Donmez, H.H., Keskin, E. and Kýsadere, I. (2012) Effects of Aflatoxin on Some Haematological Parameters and Protective Effectiveness of Esterified Glucomannan in Merino Rams. Scientific World J. 2012: 4. doi:10.1100/2012/342468.
    7. Eraslan, G., Akdogan, M., Liman, B.C., Kanbur, M., Delibas, N. (2006) Effects of dietary aflatoxin and hydrated sodium calcium aluminosilicate on triiodothyronine, thyroxin, thyrotrophin and testosterone levels in quails. Turk. J Vet. Anim. Sci. 30: 41–45.
    8. Fapohunda, S.O., Ezekiel, C.N., Alabi, O.A., Omole, A. and Chioma, S.O. (2008) Aflatoxin- mediated Sperm and Blood Cell Abnormalities in Mice Fed with Contaminated Corn. Mycobiology. 36(4): 255-259.
    9. Fink-Gremmels, J. (2008) Mycotoxins in cattle feeds and carry-over to dairy milk: A review. Food Addit Contam25: 172-180.
    10. Gajecka, M., Jakimiuk, E., Polak, M., Otrocka-Domagala, I., Janowski, T., Zwierzchowski, W., Obremski, K., Zielonka, L., Apoznanski, J., Gajecki, M. (2004) Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system. Pol. J. Vet. Sci. 7: 59-66.
    11. Gowda, N.K.S., Ledoux, D.R., Rottinghaus, G.E., Bermudez, A.J., Chen, Y.C. (2009) Antioxidant efficacy of curcuminoids from turmeric (Curcuma longa L.) powder in broiler chickens fed diets containing aflatoxin B1. Br. J. Nutr. 102: 1629–1634. 
    12. Harriet, A.M. (2003) Is indoor mold contamination a threat to health? J. Environ. Health. 62(2): 0022-0892.
    13. Hassan, A.M., Abdel-Aziem, S.H., El-Nekeety, A.A., Abdel-Wahhab, M.A. (2015) Panax ginseng extract modulates oxidative stress, DNA fragmentation and up-regulate gene expression in rats sub chronically treated with aflatoxin B1 and fumonisin B1. Cytotechnol. 67:861–871.
    14. Jovaisiene, J., Bakutis, B., Baliukoniene, V., Gerulis, G. (2016) Fusarium and Aspergillus mycotoxins effects on dairy cow health, performance and the efficacy of Anti-Mycotoxin Additive. Pol. J. Vet. Sci. 19(1): 79–87.
    15. Lakkawar, A.W., Chattopadhyay, S.K., Johri, T.S. (2004) Experimental aflatoxin B1 toxicosis in young rabbits- A clinical and patho-    anatomical study. Slov. Vet. Res. 41: 73-81.
    16. Mohammed, A.M., Metwally, N.S. (2009) Antiaflatoxicogenic activities of some aqeous plant extracts against AFB1 induced Renal and Cardiac damage. J. Pharmacol. Toxicol.. 4(1): 1-16.
    17. Murugesan, G.R., Ledoux, D.R., Naehrer, K., Berthiller, F., Applegate, T.J., Grenier, B., Phillips, T.D. and Schatzmayr, G. (2015) Prevalence and effects of mycotoxins on poultry health and performance, and recent development in mycotoxin counteracting strategies. Poult. Sci. 94:1298–1315.
    18. Naseer, O., Khan, J.A., Khan, M.S., Omer, M.O., Chishti, G.A., Sohail, M.L., Saleem, M.U. (2016) Comparative efficacy of silymarin and choline chloride (liver tonics) in preventing the effects of aflatoxin B1 in bovine calves. Pol. J. Vet. Sci. 19 (3): 545–551.
    19. Pasha, T.N. (2008) Detoxification of aflatoxin by yeast sludge in the feed of dairy cattle and its impact on increased milk production. Int. J. Agro. Vet. Med. Sci. 2: 18-26.
    20. Paterson, R.R.M. (2007) Aflatoxins contamination in chili samples from Pakistan. Food Control. 18: 817–820.
    21. Pastorelli, H., Milegen, J.V., Lovatto, P. and Montagne, L. (2012) Meta-analysis of feed intake and growth responses of growing pigs after a sanitary challenge. Animal. 6:952–961.
    22. Pleadin, J., Vuli, A., Persi, N., Skrivanko, M., Capek, B., Cvetnic, Z. (2015) Annual and regional variations of aflatoxin B1 levels seen in grains and feed coming from Croatian dairy farms over a 5-year period. Food Cont. 47: 221-225.
    23. Samuel, N., Ezrib, Y., Faraha, R., Igore, V., Husseinc, A., Rubinshteina, O., Assy, N. (2009) Acute Aflatoxicosis Resulting in Fulminant Hepatic Failure and Rhabdomyolysis. Gastroenterol. Res. 2 (1): 48-50.
    24. Sharma, V., Sharma, C., Paliwal, R., Pracheta, S.S. and Sharma, S. (2011) Ameliorative Effects of Curcuma Longa and Curcumin on Aflatoxin B1 Induced Serological and Biochemical Changes In Kidney of Male Mice. Asian J. Biochem. Pharm. Res. 1 (2): 338-351. 
    25. Thrasher, J.D. (2012) Aflatoxicosis in animals. Aflatoxins and Health, www.alpha boostjuice.com/AFLATOXICOSIS_IN_ANIMALS.pdf.
    26. Tripathi, M.K., Mondal, D., Karim, S.A. (2008) Growth, haematology, blood constituents and immunological status of lambs fed graded levels of animal feed grade damaged wheat as substitute of maize. J. Anim. Physiol. Anim. Nutr. 92: 75-85.
    27. Wangikar, P.B., Dwivedi, P., Sinha, N., Sharma, A.K., Telang, A.G. (2005) Teratogenic effects in rabbits of simultaneous exposure to ochratoxin A and aflatoxin B1 with special reference to microscopic effects. Toxicol. 215: 37-47. 
    28. Whitlow, L.W. and Hagler, Jr. W.M. (2005) Mycotoxins in dairy cattle: occurrence, toxicity, prevention and treatment. Proc. Southwest Nutrition Conference 124- 138.
    29. Wu, F. .and Khlangwiset, P (2010) Health economic impacts and cost-effectiveness of aflatoxin reduction strategies in Africa: Case studies in biocontrol and postharvest interventions. Food Addit. Contam. 27: 496-509.
    30. Wu, F. and Tritscher, A. (2011) Aflatoxins a global public health problem: World Health Organization, available at: https://orecomm.net/    wp-content/uploads/2011/12/WHO-Aflatoxin-public-health-issue.pdf .
    31. Yunus, W.A., Sulyok, M. and Böhm, J. (2015) Mycotoxin Cocktail in the Samples of Oilseed Cake from Early Maturing Cotton Varieties Associated with Cattle Feeding Problems. Toxins. 7: 2188-2197.
    32. Yarru, L.P., Settivari, R.S., Antoniou, E., Ledoux, D.R. and Rottinghaus, G.E. (2009) Toxicological and gene expression analysis of the impact of aflatoxin B1 on hepatic function of male broiler chicks. Poult. Sci. 88:360–371. 
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