Conventional and molecular diagnosis of haemo-protozoan infections in cattle and equids from Republic of Guinea and India

DOI: 10.18805/ijar.v0iOF.6988    | Article Id: B-599 | Page : 1206-1211
Citation :- Conventional and molecular diagnosis of haemo-protozoan infectionsin cattle and equids from Republic of Guinea and India .Indian Journal of Animal Research.2018.(52):1206-1211

Talibe Diallo, L.D. Singla, Deepak Sumbria, Paramjit Kaur and M.S. Bal

Address :

Department of Veterinary Parasitology, College of Veterinary Sciences, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141 004, Punjab, India.

Submitted Date : 16-08-2016
Accepted Date : 4-10-2016


A cross-sectional study was carried out on 50 N’ Dama cattle and 35 equids blood samples from Republic of Guinea and Punjab, India, respectively to assess the level of exposure to obligatory haemoprotozoa Theileria equi and Trypanosoma evansi by 18S rRNA gene based primary and nested polymerase chain reaction (nPCR) and RoTat1.2 based card agglutination test (CATT), and to investigate risk factors associated with the infection. Blood smear examination revealed the prevalence rate of 6% (95% CI = 2.06-16.22) for Trypanosoma spp. in N’dama cattle and 5.7% for T. equi in equids. In equids, 17.14% (95% CI = 8.10-32.68) samples showed positive titer by CATT. Primary PCR showed 5.71% (95% CI = 1.58-18.8) infection and on the other hand nested PCR depicted 20% (95% CI = 10.04-35.89) T. equi infection. Moreover, only 8.57% (95% CI = 2.96-22.38) prevalence of T. evansi was recorded by T. evansi based multiplex PCR. PCRs revealed higher risk of infection of both T. equi (OR = 5.28, 95% CI = 0.68-49.81) and T. evansi (OR = 3.33, 95% CI = 0.20-104.83) in the farms where proper deworming/vaccination schedule was not followed. The risk factor associated with the type of host species had an odds ratio (OR) of 5 (95% CI = 3.90-74.33) for donkeys/mules versus horses for T. equi infection. This group was also at higher risk of infection with OR of 4.8 (95% CI = 0.12-124.47) for T. evansi. The present exploration brings out a variety of commodities at risk of infectivity pertaining to trypanosomosis and theileriosis calculated by different PCRs assay.


Card agglutination Test Cattle Equids Polymerase chain reaction Theileria equi Trypanosoma spp.


  1. Altschul, S.F., Gish, W., Miller, W., Myers, E.W. and Lipman, D.J.(1990). Basic local alignment search tool. J. Mol. Biol. 215: 403-410.
  2. Baptista, C., Lopes, M.S., Tavares, A.C., Rojer, H., Kappmeyer, L., Mendon, D. and Machado, A.D.C.(2013). Diagnosis of Theileria equi infections in horses in the Azores using cELISA and nested PCR. Ticks Tick Borne Dis.4: 242–245.
  3. Chhabra, S., Ranjan, R., Uppal, S.K. and Singla, L.D.(2011). Transplacental transmission of Babessia equi (Theileria equi) from carrier mares to foals. J. Parasit Dis. Doi: 10, 1007/s 12639-011-0072-1.
  4. Coles,E.H. (1986). Veterinary Clinical Pathology, 4th ed. W.B. Saunders Co, Philadel-phia.
  5. Cordon-Obras, C., Garci´a-Este´banez, C., Ndong-Mabale, N., Abaga, S., Ndongo-Asumu, P., Benito, A. and Cano, J. (2010). Screening of Trypanosoma brucei gambiense in Domestic Livestock and Tsetse Flies from an Insular Endemic Focus (Luba, Equatorial Guinea). Plos neglected Trop.Dis. 4: e704.
  6. Dos Santos, M.T., Roier, E.C., Santos, H.A., Pires, M.S., Vilela, J.A., Moraes, L.M., Almeida, F.Q., Baldani, C.D., Machado, R.Z. and Massard, C.L.(2011). Factors associated to Theileria equi in equids of two micro-regions from Rio de Janeiro, Brazil. Rev. Bras. Parasitol Vet. Jaboticabal 20: 235-241.
  7. Herbert, W.J. and Lumsden, W.H.R.(1976).Trypanosoma brucei: a rapid ‘matching’method for estimating the host parasitaemia. Exp. Parasitol.40: 427-431.
  8. Hoare, C.A.(1972).The Trypanosomes of mammals, In: Zoological Monograph. Black Scientific Publication, Oxford, pp 749.
  9. Juyal PD, Singla LD and Kaur P (2005). Management of surra due to Trypanosoma evansi in India: an overview. In: Infectious Diseases of Domestic Animals and Zoonosis in India, [Tandon V and Dhawan BN (Eds)], Proceedings of the National Academy of Sciences India Section B: Biological Science 75 (Special issue): 109-120.
  10. Kumar, S., Malhotra, D.V., Sangwan, A.K., Goel, P. and Kumar, A.(2007). Infectivity rate and transmission potential of Hyalomma anatolicum anatolicum ticks for Babesia equi infection. Vet. Parasitol.144: 338-343.
  11. Muieed, M.A., Chaudhary, Z.I. and Shakoori, A.R.(2010). Comparative studies on the sensitivity of polymerase chain reaction (PCR) and microscopic examination for the detection of Trypanosoma evansi in horses. Turk. J. Vet. Anim. Sci.34: 507-512.
  12. Rampersad, J., Cesar, E., Campbell, M.D., Samlal, M. and Ammons, D.(2003). A field evaluation of PCR for the routine detection of Babesia equi in horses. Vet. Parasitol.114: 81-87.
  13. Ribeiro, I.B., Câmara, A.C.L., Bittencourt, M.V., Marçola, T.G., Paludo, G.R. and Soto-Blanco, B. (2013). Detection of Theileria equi in spleen and blood of asymptomatic piroplasm carrier horses. Acta Parasitol.58: 218-222.
  14. Sharma, A., Singla, L.D., Tuli, A., Kaur,P., Batth, B.K., Javed, M. and Juyal, P.D.(2013). Molecular Prevalence of Babesia bigemina and Trypanosoma evansi in Dairy Animals from Punjab, India, by Duplex PCR: A Step Forward to the Detection and Management of Concurrent Latent Infections. BioMed Res. Int. 1-8.
  15. Singla, L.D., Juyal, P.D. and Sharma, N.S. (2010). Immune responses to haemorrhagic septicaemia (HS) vaccination in Trypanosoma evansi infected buffalo-calves. Trop. Anim. Health Prod. 42: 589–595.
  16. Sumbria, (2015). Studies on diagnosis and haematobiochemical alterations in theileriosis of equines in Punjab. Dissertation submitted to the Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India in partial fulfillment of the requirement for the degree of Doctor of Philosophy in Veterinary Parasitology.
  17. Sumbria, D., Singla, L.D. and Sharma, A.(2016b).Theileria equi and Babesia caballi infection of equids in Punjab, India: a serological and molecular survey. Trop. Anim. Health Prod.48:45-52.
  18. Sumbria, D., Singla, L.D., Kumar, S., Sharma, A., Dahiya, R.K. and Setia, R.(2016a). Spatial distribution, risk factors and haematobiochemical alterations associated with Theileria equi infected equids of Punjab (India) diagnosed by indirect ELISA and nested PCR. Acta Trop.155: 104-112.
  19. Sumbria, D., Singla, L.D., Mandhotra, A. and Randhawa, C.S.(2015a).Molecular Detection and Treatment of Equine Piroplasmosis. Philipp. J. Vet. Med.52: 131-136.
  20. Sumbria, D., Singla, L.D., Sharma, A., Bal, M.S. and Kumar, S. (2015b). Multiplex PCR for detection of Trypanosoma evansi and Theileria equi in equids of Punjab, India. Vet. Parasitol. 211: 293-299.
  21. Sumbria, D., Singla, L.D., Sharma, A., Moudgil, A.D. and Bal, M.S.(2014). Equine trypanosomosis in central and western Punjab: Prevalence, haemato-biochemical response and associated risk factors. Acta Trop.138: 44-50.
  22. Swallow, B.M. (1997). Impacts of trypanosomosis on Africa agriculture. ILRI, review paper prepared for the Programme against African Trypanosomosis (PAAT) for the presentation at the ISCTRC, Maputo, Mozambique, 4: 46.
  23. Verloo, D., Magnus, E. and Büscher, P. (2001). General expression of RoTat 1.2 variable antigen type in Trypanosoma evansi isolates from different origin. Vet. Parasitol. 97: 183-189.

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